Prediction of immune disorders by evaluation of serum IgG reactivity to random peptide library

• Project/Area Number: 22659057
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: TAKAI Toshiyuki 東北大学, 加齢医学研究所, 教授 (20187917)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,010,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 免疫制御 / 抗体 / 自己免疫疾患 / アレルギー / 炎症 / マイクロアレイ / ランダムペプチド / 予防診断技術 / 自然抗体 / プロファイリング

Research Abstract

Based on an assumption that reactivities of natural antibodies in sera will be changed before and after onset of immune disorders such as autoimmune diseases, we first analyzed the effect of human polyclonal IgG preparations on immune cell functions such as that of B cells. We found that F(ab')_2 fragment of IgG can bind to various cell surfaces and swiftly internalized into the cytosol. Interestingly, the polyclonal IgG was able to suppress cytokine production from B cells stimulated with CpG.

Research Products

• [Journal Article] Presumptive role of 129 strain-derived Sle16 locus in rheumatoid arthritis in a new mouse model with Fcy receptor type Iib-deficient C57BL/6 genetic background. (2011)
  • Author(s): Sato-Hayashizaki A, Ohtsuji M, Lin Q, Hou R, Ohtsuji N, Nishikawa K, Tsurui H, Sudo K, Ono M, Izui S, Shirai T, Takai T, Nishimura H, Hirose S
  • Journal Title: Arthritis & Rheumatism Volume: 63 Issue: 10 Pages: 2930-2938
  • DOI: 10.1002/art.30485
  • Peer Reviewed
• [Journal Article] Interaction between NK cells and dendritic cells generate MHC class II-dressed NK cells to regulate CD4^+ T cells (2011)
  • Author(s): Nakayama M, et al
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: 108 Issue: 45 Pages: 18360-18365
  • DOI: 10.1073/pnas.1110584108
  • Peer Reviewed
• [Journal Article] Control of pathogenic CD4 T cells and lethal immunopathology by signaling immunoadaptor DAP12 during influenza infection (2011)
  • Author(s): McCormick S, Shaler CR, Small CL, Horvath C, Damjanovic D, Brown EG, Aoki N, Takai T, Xing Z
  • Journal Title: J Immunol Volume: 187(8) Issue: 8 Pages: 4280-92
  • DOI: 10.4049/jimmunol.1101050
  • Peer Reviewed
• [Journal Article] The p75 receptor mediates axon growth inhibition through an association with PIR-B (2011)
  • Author(s): Fujita Y, et al
  • Journal Title: Cell Death Dis. Volume: 2 Issue: 9 Pages: e198-e198
  • DOI: 10.1038/cddis.2011.85
  • Peer Reviewed
• [Journal Article] Differential but competitive binding of Nogo and MHCI to PIR-B provides an inhibition of cells (2011)
  • Author(s): Matsushita H, et al
  • Journal Title: J.Biol.Chem. Volume: 286(29) Issue: 29 Pages: 25739-25747
  • DOI: 10.1074/jbc.m110.157859
  • Peer Reviewed
• [Journal Article] Transcriptional activation of the Pirb gene in B cells by PU.1 and Runx3 (2011)
  • Author(s): Arita K, et al
  • Journal Title: J.Immunol. Volume: 186(12) Issue: 12 Pages: 7050-7059
  • DOI: 10.4049/jimmunol.1001302
  • Peer Reviewed
• [Journal Article] Mycobacterial hypersensitivity pneumonitis requires TLR9-MyD88 in lung CD11b+ CD11c+ cells (2011)
  • Author(s): Daito H, Kikuchi T, Sakakibara T, Gomi K, Damayanti T, Zaini J, Tode N, Kanehira M, Koyama S, Fujimura S, Ebina M, Ishii KJ, Akira S, Takai T, Watanabe A, Nukiwa T
  • Journal Title: Eur Respir J Volume: 38 Issue: 3 Pages: 688-701
  • DOI: 10.1183/09031936.00177110
  • Peer Reviewed
• [Journal Article] Paired immunoglobulin like receptor-B regulates the suppressive function and fate of myeloid derived suppressor cells (2011)
  • Author(s): Ma G, Pan PY, Eisenstein S, Divino CM, Lowell C, Takai T, Chen SH
  • Journal Title: Immunity Volume: 34 Issue: 3 Pages: 385-395
  • DOI: 10.1016/j.immuni.2011.02.004
  • Peer Reviewed
• [Journal Article] Myelin suppresses axon regeneration by PIR-B/SHP-mediated inhibition of Trk activity (2011)
  • Author(s): Fujita Y, Endo S, Takai T, Yamashita T
  • Journal Title: EMBO J Volume: 30(7) Pages: 1389-401
  • Peer Reviewed
• [Journal Article] An immunoreceptor adapter protein, DAP12, suppresses adaptive immune responses mediated by B lymphocytes (2011)
  • Author(s): Nakano-Yokomizo T, Tahara-Hanaoka S, Nakahashi-Oda C, Nabekura T, Tchao NK, Kadosaki M, Totsuka N, Kurita N, Nakamagoe K, Tamaoka A, Takai T, Yasui T, Kikutani H, Honda S, Shibuya K, Lanier LL, Shibuya A
  • Journal Title: J. Exp. Med Volume: (in press)
  • Peer Reviewed
• [Journal Article] Myelin suppresses axon regeneration by PIR-B/SHP-mediated inhibition of Trk activity (2011)
  • Author(s): Fujita Y, et al
  • Journal Title: EMBO J. Volume: 6;30(7) Issue: 7 Pages: 1389-1401
  • DOI: 10.1038/emboj.2011.55
  • Peer Reviewed
• [Journal Article] Paired immunoglobulin like receptor-B regulates the suppressive function and fate of myeloid derived suppressor cells. (2011)
  • Author(s): Ma G, et al.
  • Journal Title: Immunity Volume: 34 Pages: 385-395
  • Peer Reviewed
• [Journal Article] Myelin suppresses axon regeneration by PIR-B/SHP-mediated inhibition of Trk activity. (2011)
  • Author(s): Fujita Y, et al.
  • Journal Title: EMBOJ Volume: In press
  • Peer Reviewed
• [Journal Article] Paired immunoglobulin-like receptor B knockout does not enhance axonal regeneration or locomotor recovery after spinal cord injury. (2011)
  • Author(s): Nakamura, Y, et al.
  • Journal Title: J.Biol.Chem. Volume: In press
  • Peer Reviewed
• [Journal Article] The inhibitory effects of intravenous administration of rabbit IgG on air way inflammation are dependent on Fcgamma receptor IIb on CD11c+dendritic cells in murine model. (2011)
  • Author(s): Yamamoto M, et al.
  • Journal Title: Clin.Exp.Immunol. Volume: In press
  • Peer Reviewed
• [Journal Article] Paired immunoglobulin-like receptor B knockout does not enhance axonal regeneration or locomotor recovery after spinal cord injury (2010)
  • Author(s): Nakamura, Y., Fujita, Y., Ueno, M., Takai, T. Yamashita, T.
  • Journal Title: Biol. Chem Volume: 286(3) Pages: 1876-83
  • Peer Reviewed
• [Journal Article] Role of PIR-B in Autoimmune Glomerulonephritis (2010)
  • Author(s): Takai T, Nakamura A, Endo S.
  • Journal Title: J. Biomed. Biotech Volume: vol.2011 Pages: 275302-275302
• [Journal Article] Genetic deletion of paired immunoglobulin-like receptor B does not promote axonal plasticity or functional recovery after traumatic brain injury (2010)
  • Author(s): Omoto S, Ueno M, Mochio S, Takai T, Yamashita T
  • Journal Title: J. Neurosci Volume: 30 Pages: 13045-13052
  • Peer Reviewed
• [Journal Article] The inhibitory effects of intravenous administration of rabbit IgG on airway inflammation are dependent on Fcgamma receptor IIb on CD11c+ dendritic cells in murine model (2010)
  • Author(s): Yamamoto M, Kobayashi K, Ishikawa Y, Nakata K, Funada Y, Kotani Y, Masuda A, Takai T, Azuma T, Yoshida M, Nishimura Y
  • Journal Title: Clin. Exp. Immunol Volume: 162(2) Pages: 315-24
  • Peer Reviewed
• [Journal Article] TIM1 is an endogenous ligand for LMIR5/CD300b : LMIR5 deficiency ameliorates mouse kidney 1ischemia/reperfusion injury (2010)
  • Author(s): Yamanishi Y, Kitaura J, Izawa K, Kaitani A, Komeno Y, Nakamura M, Yamazaki S, Enomoto Y, Oki T, Akiba H, Abe T, Komori T, Morikawa Y, Kiyonari H, Takai T, Okumura K, Kitamura T
  • Journal Title: J. Exp. Med Volume: 207(7) Pages: 1501-11
  • Peer Reviewed
• [Journal Article] Genetic deletion of paired immunoglobulin-like receptor B does not promote axonal plasticity or functional recovery after traumatic brain injury. (2010)
  • Author(s): Omoto S, et al.
  • Journal Title: J.Neurosci. Volume: 30 Pages: 13045-13052
  • Peer Reviewed
• [Journal Article] TIM1 is an endogenous ligand for LMIR5/CD300b: LMIR5 deficiency a meliorates mouse kidney ischemia/reperfusion injury. (2010)
  • Author(s): Yamanishi Y, et al.
  • Journal Title: J.Exp.Med. Volume: 207(7) Pages: 1501-1511
  • Peer Reviewed
• [Presentation] Regulatory effects of IgG on B cell functions (2012)
  • Author(s): 高井俊行
  • Organizer: RCAI Workshop
  • Place of Presentation: RCAI, Yokohama
  • Year and Date: 2012-03-23
• [Presentation] Update understanding of the mechanism of action of IVIG (2012)
  • Author(s): Toshiyuki Takai
  • Organizer: 10th Kawasaki Disease Symposium
  • Place of Presentation: Kyoto
  • Year and Date: 2012-02-08
• [Presentation] Update understanding of the mechanism of action of IVIG (2012)
  • Author(s): Toshiyuki Takai
  • Organizer: 10^<th> Kawasaki Disease Symposium
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2012-02-08
• [Presentation] Regulation of TLR9 and autoimmunity by PirB, a multiligand inhibitory receptor (2011)
  • Author(s): Toshiyuki Takai
  • Organizer: Second CSI/JSI/KAI Joint Symposium on Immunology"Regulation of Immune Responses in Health and Diseases
  • Place of Presentation: Osaka
  • Year and Date: 2011-12-06
• [Presentation] Regulation of TLR9 and autoimmunity by PirB, a multiligand inhibitory receptor (2011)
  • Author(s): Toshiyuki Takai
  • Organizer: Second CSI/JSI/KAI Joint Symposium on Immunology
  • Place of Presentation: 大阪(招待講演)
  • Year and Date: 2011-12-06
• [Presentation] Differential but competitive binding of Nogo and MHC class I to PIR-B regulates LPS-activated mast cells (2011)
  • Author(s): Shinozaki N, Inui M, Endo S, Sakamoto Y, Kitaguchi K, Kuroki K, Maenaka K, Takai T
  • Organizer: 2011 The annual meeting of the Japanese society for immunology
  • Place of Presentation: Chiba
  • Year and Date: 2011-11-29
• [Presentation] Transcriptional activation of the PIR-B gene in B cells by PU. 1 and Runx3 (2011)
  • Author(s): Kitaguchi K, Arita K, Endo S, Kaifu T, Nakamura A, Takai T
  • Organizer: 2011 The annual meeting of the Japanese society for immunology
  • Place of Presentation: Chiba
  • Year and Date: 2011-11-28
• [Presentation] A suppressive effect of IVIg on B1 cell activation and production of natural antibodies (2011)
  • Author(s): Hirano K, Tanaka J, Sakamoto Y, Looi CY, Nakano A, Itoh Y, Takai T
  • Organizer: The annual meeting of the Japanese society for immunology
  • Place of Presentation: Chiba
  • Year and Date: 2011-11-27
• [Presentation] Fcγ receptor targeting . How ITIM receptors regulate (2011)
  • Author(s): Toshiyuki Takai
  • Organizer: Bio-Rheumatology International Congress (BRIC)Tokyo/The 8th GARN Meeting Locomotor Science 1 . Recent Topics of RA
  • Place of Presentation: Hilton Tokyo Bay Hotel
  • Year and Date: 2011-11-15
• [Presentation] Fcγ receptor targeting-How ITIM receptors regulate (2011)
  • Author(s): Toshiyuki Takai
  • Organizer: Bio-Rheumatology International Congress (BRIC)
  • Place of Presentation: 幕張(招待講演)
  • Year and Date: 2011-11-15
• [Presentation] NogoおよびMHC class IによるPIR-Bを介した新たなマスト細胞の制御機構 (2011)
  • Author(s): 乾匡範, 篠崎夏子, 遠藤章太, 高井俊行
  • Organizer: 第61回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-11
• [Presentation] NogoおよびMHCクラスIによるPIR-Bへの競合的結合によるマスト細胞の制御について (2011)
  • Author(s): 篠崎夏子, 乾匡範, 遠藤章太, 坂本譲, 北口公司, 高井俊行
  • Organizer: 第61回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-11
• [Presentation] 抗体療法とFcレセプター (2011)
  • Author(s): 高井俊行
  • Organizer: 第31回日本川崎病学会・学術集会ランチョンセミナー2
  • Place of Presentation: はまぎんホール・ヴィアマーレ横浜市
  • Year and Date: 2011-10-01
• [Presentation] 抗体療法とFcレセプター (2011)
  • Author(s): 高井俊行
  • Organizer: 第31回日本川崎病学会・学術集会
  • Place of Presentation: 横浜(招待講演)
  • Year and Date: 2011-10-01
• [Presentation] Intravenous Immunoglobulin inhibits autoantibody production by B-1 cells (2011)
  • Author(s): Tanaka J, Nakano A, Itoh Y and Takai T
  • Organizer: 10th World Congress on Inflammation
  • Place of Presentation: Paris, France
  • Year and Date: 2011-06-26
• [Presentation] Fc受容体と免疫疾患について (2011)
  • Author(s): 高井俊行
  • Organizer: 第16回シェーグレン症候群セミナー
  • Place of Presentation: 東京
  • Year and Date: 2011-06-04
• [Presentation] Fc受容体と免疫疾患について (2011)
  • Author(s): 高井俊行
  • Organizer: 第16回シェーグレン症候群セミナー特別講演
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-06-04
• [Presentation] 静注用免疫グロブリンはB-1細胞からの自己抗体産生を抑制する (2011)
  • Author(s): 田中純, 仲野篤史, 伊藤由美, 高井俊行
  • Organizer: 第32回日本炎症・再生医学会
  • Place of Presentation: 京都
  • Year and Date: 2011-06-03
• [Presentation] Targeting Fc receptors (2011)
  • Author(s): Toshiyuki Takai
  • Organizer: 第32回日本炎症・再生医学会
  • Place of Presentation: 京都
  • Year and Date: 2011-06-02
• [Presentation] Targeting Fc receptors (2011)
  • Author(s): Toshiyuki Takai
  • Organizer: 第32回日本炎症・再生医学会
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2011-06-02
• [Presentation] 制御性受容体PIR・Bによる自己免疫の制御 (2011)
  • Author(s): 高井俊行
  • Organizer: 愛媛大学プロテオ医学研究センター学術シンポジウム
  • Place of Presentation: 愛媛県松山市
  • Year and Date: 2011-02-06
• [Presentation] Regulation of TLR9 activation and autoantibody production in B-1 cells by PIR-B, an MHC class I receptor (2010)
  • Author(s): 高井俊行
  • Organizer: 8th German-Japanese Symposium.
  • Place of Presentation: Cuxhaven, Germany
  • Year and Date: 2010-09-28
• [Presentation] Regulation of autoimmunity by PIR-B. (2010)
  • Author(s): 高井俊行
  • Organizer: Asian Aging Core for Longevity 2010
  • Place of Presentation: Jeju island,Korea
  • Year and Date: 2010-08-25
• [Book] 骨髄球系細胞におけるMDL-1/DAP12の機能 (2011)
  • Author(s): 北口公司, 遠藤章太, 乾匡範, 高井俊行
  • Publisher: 科学評論社
• [Book] Paired immunoglobulin-like receptor(PIR)-Bの自己免疫疾患における役割 (2011)
  • Author(s): 中村晃, 高井俊行
  • Publisher: 科学評論社
• [Remarks]
  • URL: http://www2.idac.tohoku.ac.jp/dep/expimmu/