Optimization and standardization of cultivation for human mesenchymal stem cells derived from differential tissues

• Project/Area Number: 22659076
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Human pathology
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: HIDA Naoko 独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 研究員 (70360112)
• Co-Investigator(Kenkyū-buntansha): UMEZAWA Akihiro 独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 部長(再生医療センター長) (70213486) ; TOYODA Masashi 地方独立行政法人東京都健康長寿医療センター, 研究所老年病研究チーム・血管医学研究, 研究副部長 (50392486)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,200,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥600,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥600,000 (Direct Cost: ¥600,000)
• Keywords: ES細胞 / 遺伝子解析 / 再生医療 / 細胞移植 / 移植・再生医療 / 再生医学 / 細胞プロファイリング / 間葉系幹細胞 / 心筋分化 / 間葉系細胞 / 共培養 / プロファイリング / 細胞リソース / 虚血性心疾 / ヒト幹細胞 / バイオリソース

Research Abstract

It has been noted that human mesenchymal stem cells are one of the most suitable cell sources. Mesenchymal stem cells exist in many tissues and differ in their property. In this study, we optimized the cultivation system for clinical purpose. We will improve efficacies of the cell products through information sharing.

Research Products

• [Journal Article] Treatment of human mesenchymal stem cells withangiotensin receptor blocker improved efficiency of cardiomyogenic trans differentiation and improved cardiac function via angiogenesis. (2011)
  • Author(s): Numasawa Y, Kimura T, Miyoshi S, Nishiyama N, Hida N, Tsuji H, Tsuruta H, Segawa K, Ogawa S, Umezawa A
  • Journal Title: Stem Cells Volume: 29(9) Pages: 1405-14
• [Journal Article] Pretreatment of human mesenchymal stemcells with pioglitazone improved efficiency of cardiomyogenictransdifferentiation and cardiac function. (2011)
  • Author(s): Shinmura D, Togashi I, Miyoshi S, Nishiyama N, Hida N, Tsuji H, Tsuruta H,Segawa K, Tsukada Y, Ogawa S, Umezawa A
  • Journal Title: Stem Cells Volume: 29(2) Pages: 357-66
• [Journal Article] Pretreatment of Human Mesenchymal Stem Cells with Pioglitazone Improved Efficiency of Cardiomyogenic Transdifferentiation and Improved Cardiac Function (2010)
  • Author(s): Shinmura D., Togashi I., Miyoshi S., Nishiyama N., Hida N., Tsuji H., Tsuruta H., Nakamizo H., Segawa K., Tsukada Y., Ogawa S., Umezawa A.
  • Journal Title: Stem Cells. Volume: 29 Pages: 357-366
  • Peer Reviewed
• [Journal Article] Xenografted human amniotic membrane-derived mesenchymal stem cells are immunologically tolerated and transdifferentiated into cardiomyocytes (2010)
  • Author(s): Tsuji H, Miyoshi S, Ikegami Y, Hida N, Asada H, Togashi I, Suzuki J, Satake M, Nakamizo H, Tanaka M, Mori T, Segawa K, Nishiyama N, Inoue J, Makino H, Miyado K, Ogawa S, Yoshimura Y, Umezawa A.
  • Journal Title: Circ Res. Volume: 28;106(10) Pages: 1613-1623
  • Peer Reviewed
• [Journal Article] Serum-independent cardiomyogenic transdifferentiation in human endome trium-derived mesenchymal cells. (2010)
  • Author(s): Ikegami Y, Miyoshi S, Nishiyama N, Hida N, Okamoto K, Miyado K, Segawa K, Ogawa S, Umezawa A.
  • Journal Title: Artif Organs. Volume: Apr;34(4) Pages: 280-288
  • Peer Reviewed
• [Presentation] ピオグリダゾン活性化骨髄間葉系幹細胞移植がイヌ心筋梗塞モデルで心機能を改善する (2010)
  • Author(s): 高橋辰郎、古梶清和、三好俊一郎、岡本一真、石田治、木村雄弘、宗形昌儒、村木浩司、稲川浩平、肥田直子、西山信大、新村大輔、梅澤明弘、四津良平
  • Organizer: 第63回日本胸部外科学会定期学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-24