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Mechanisms of bone metastasis of tumor cells by endothelial-mesenchymal transition of vascular endothelial cells.

Research Project

Project/Area Number 22659078
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionHokkaido University

Principal Investigator

TSUDA Masumi  北海道大学, 大学院・医学研究科, 助教 (30431307)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,090,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywords血管内皮細胞 / 内皮間葉移行 / 腫瘍微小環境 / 骨転移 / 腫瘍血管新生
Research Abstract

We clarified that human synovial sarcoma and oral cancer cells induced vascular endothelial cells into the tumors by Src-dependent VEGF production and oral environment specific induction of RANKL, respectively. In addition, using in vitro 3D culture system we have established, synovial sarcoma cells aggressively invaded into the tubes formed by HUVECs, leading to the morphological alterations like endothelialmesenchymal transition in HUVECs, resulted in the aberrant tube formation combined with synovial sarcoma cells.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (28 results)

All 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 7 results) Presentation (18 results) Remarks (1 results)

  • [Journal Article] Simultaneous inhibition of Src and Aurora kinases by SU6656 induces therapeutic synergy in human synovial sarcoma growth, invasion and angiogenesis in vivo.2012

    • Author(s)
      Arai R, Tsuda M, Watanabe T, Ose T, Obuse C, Maenaka K, Minami A, Ohba Y.
    • Journal Title

      Eur J Cancer

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous inhibition of Src and Aurora kinases by SU6656 induces therapeutic synergy in human synovial sarcoma growth, invasion and angiogenesis in vivo2012

    • Author(s)
      Arai R, Tsuda M, Watanabe T, Ose T, Obuse C, Maenaka K, Minami A, Ohba Y
    • Journal Title

      Eur J Cancer

      Volume: (Epub ahead of print)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosensors for BCR-ABL activity and their application to cancer2012

    • Author(s)
      Ohba Y, Darmanin S, Mizutani T, Tsuda M, Kondo T
    • Journal Title

      Biosensors and Cancer

      Pages: 268-283

    • Related Report
      2011 Annual Research Report
  • [Journal Article] RANKL expression specifically observed in vivo promotes epithelial mesenchymal transition and tumor progression2011

    • Author(s)
      Yamada T, Tsuda M, Takahashi T, Totsuka Y, Shindoh M, Ohba Y.
    • Journal Title

      Am J Pathol

      Volume: 178 Pages: 2845-56

    • NAID

      120005367443

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Ras-PI3K signaling pathway is involved in clathrin-independent endocytosis and the internalization of influenza viruses.2011

    • Author(s)
      Fujioka Y, Tsuda M, Hattori T, Sasaki J, Sasaki T, Miyazaki T, Ohba Y.
    • Journal Title

      PLoS One

      Volume: 6

    • NAID

      120002834740

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel FRET-based biosensor for the measurement of BCR-ABL activity and its response to drugs in living cells2010

    • Author(s)
      Mizutani T, Kondo T, Darmanin S, Tsuda M, Tanaka S, Tobiume M, Asaka M, Ohba Y.
    • Journal Title

      Clin Cancer Res

      Volume: 16 Pages: 3964-3975

    • NAID

      120003207587

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel FRET-based biosensor for the measurement of BCR-ABL activity and its response to drugs in living cells.2010

    • Author(s)
      Mizutani T, Kondo T, Darmanin S, Tsuda M, Tanaka S, Tobiume M, Asaka M, Ohba Y.
    • Journal Title

      Clin Cancer Res.

      Volume: 16 Pages: 3964-3975

    • NAID

      120003207587

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞内シグナル伝達の可視化技術と分子標的治療薬の耐性判定への応用

    • Author(s)
      大場雄介、津田真寿美
    • Journal Title

      生化学(ミニレビュー)

      Volume: (in press) Pages: 2012-2012

    • NAID

      10030741585

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Functional Biomarkers of Oral Cancer

    • Author(s)
      Tsuda M, Ohba Y
    • Journal Title

      Oral Cancer

      Volume: (in press) Pages: 2012-2012

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] RANKL upregulates integrinα2 expression and cell adhesion in oral cancer cells2011

    • Author(s)
      Wagatsuma T, Yamada T, Tsuda M, Fujioka Y, Kaibara T, Totsuka Y, Shindoh M, Ohba Y.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Involvement of RhoA-PIP5K-mediated calcium signaling in influenza virus entry and infection2011

    • Author(s)
      Fujioka Y, Tsuda M, Hattori T, Sasaki J, Sasaki T, Miyazaki T, Ohba Y
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] RANKL upregulates integrin α2 expression and cell adhesion in oral cancer cells2011

    • Author(s)
      Wagatsuma T, Yamada T,Tsuda M, Fujioka Y, Kaibara T, Totsuka Y, Shindoh M, Ohba Y
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] SU6656 suppresses human synovial sarcoma progression through Src and Aurora kinase inhibition2011

    • Author(s)
      Arai R, Tsuda M, Watanabe T, Ose T, Obuse C, Maenaka K, Minami A, Ohba Y.
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 慢性骨髄性白血病薬剤耐性細胞の分離と解析2011

    • Author(s)
      金安顕子、近藤健、ダルマニンステファニー、津田真寿美、大場雄介
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] SU6656 suppresses human synovial sarcoma progression through Src and Aurora kinase inhibition2011

    • Author(s)
      Arai R, Tsuda M, Watanabe T, Ose T, Obuse C, Maenaka K, Minami A, Ohba Y
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dual inhibition of Src and Aurora kinases abrogates tumor growth, invasion, and angiogenesis of synovial sarcoma in vivo2011

    • Author(s)
      Tsuda M, Arai R, Watanabe T, Ose T, Obuse C, Maenaka K, Minami A, Ohba Y.
    • Organizer
      第70回日本癌学会総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-10-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Involvement of signaling adaptor molecule CRK and its related proteins in various human cancers2011

    • Author(s)
      Tanaka S, Wang L, Tsuda M, Roshan M, Aiman Z, Yanagi H, Makino Y, Tanino M, Kimura T, Nishihara H
    • Organizer
      第70回日本癌学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Seeing response and resistance to BCR-ABL inhibition in chronic myeloid leukemia2011

    • Author(s)
      Ohba Y, Kaneyasu A, Mizutani T, Darmanin S, Kondo T, Tsuda M
    • Organizer
      第70回日本癌学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dual inhibition of Src and Aurora kinases abrogates tumor growth, invasion, and angiogenesis of synovial sarcoma in vivo2011

    • Author(s)
      Tsuda M, Arai R, Watanabe T, Ose T, Obuse C, Maenaka K, Minami A, Ohba Y
    • Organizer
      第70回日本癌学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Influenza viruses activates Ras-PI3K-mediated endocytosis via calcium signaling2011

    • Author(s)
      藤岡容一朗、津田真寿美、服部ともえ、佐々木純子、佐々木雄彦、宮崎忠昭、大場雄介
    • Organizer
      第63回日本細胞生物学会
    • Place of Presentation
      北海道大学学術交流会館(札幌市)
    • Year and Date
      2011-06-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] SU6656はAurora kinaseを標的とすることによりG2/M進行を阻害する2011

    • Author(s)
      津田真寿美、新井隆太、大場雄介
    • Organizer
      第63回日本細胞生物学会
    • Place of Presentation
      北海道大学学術交流会館(札幌市)
    • Year and Date
      2011-06-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] RANKLは口腔癌細胞のインテグリンα2の発現と細胞接着を亢進する2011

    • Author(s)
      大場雄介、山田珠希、藤岡容一朗、甲斐原拓真、戸塚泰則、進藤正信、津田真寿美
    • Organizer
      第63回日本細胞生物学会
    • Place of Presentation
      北海道大学学術交流会館(札幌市)
    • Year and Date
      2011-06-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 受容体サブタイプ間相互作用によるAngIIシグナル制御機構2010

    • Author(s)
      大場雄介、犬塚(鈴木)貴之、津田真寿美、川口秀明
    • Organizer
      第38回薬物活性シンポジウム
    • Place of Presentation
      札幌市教育文化会館
    • Year and Date
      2010-11-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] RANKL発現は腫瘍形成とEMTを亢進する2010

    • Author(s)
      山田珠希、津田真寿美、戸塚泰則、進藤正信、大場雄介
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2010-09-24
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] RANKLは微少環境特異的に発現し細胞接着・EMT・腫瘍形成を促進する生体内特異的機能マーカーである2010

    • Author(s)
      山田珠希、津田真寿美、進藤正信、戸塚泰則、大場雄介
    • Organizer
      第62回日本細胞生物学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2010-05-20
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Ras-PI3Kシグナル経路がエンドサイトーシスとインフルエンザ感染を調節する2010

    • Author(s)
      藤岡容一朗、津田真寿美、服部ともえ、佐々木純子、佐々木雄彦、宮崎忠昭、大場雄介
    • Organizer
      第62回日本細胞生物学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2010-05-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] エンドソームにおけるアンジオテンシン2型受容体による1型受容体シグナリングの抑制機構2010

    • Author(s)
      津田真寿美、犬塚-鈴木貴之、川口秀明、大場雄介
    • Organizer
      第62回日本細胞生物学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2010-05-19
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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