Development of early diagnostic method for Deep mycoses by monitoring volatile organic compounds
| Project/Area Number |
22659086
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Nara Women's University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Takae 奈良女子大学, 自然科学系, 准教授 (80201606)
SUZUKI Takahito 奈良女子大学, 自然科学系, 教授 (60144135)
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| Co-Investigator(Renkei-kenkyūsha) |
YOKOYAMA Koji 千葉大学, 真菌医学研究センター, 准教授 (80092112)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,060,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | 診断 / 真菌感染症 / 深在性真菌症 / アスペルギルス症 / 揮発性有機化合物 / ガスクロマトグラフィー質量分析計 / 動物感染実験モデル / 感染症 / 菌類 / 微生物 / 病理学 / 動物真菌感染モデル / 真菌 / 動物モデル |
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| Research Abstract |
We evaluated the availability of VOCs (volatile organic compounds) as the biomarker during the pulmonary invasive aspergillosis in murine infection model. We established the murine infection model and developed the apparatus for trapping of VOCs in murine breath. The detection of VOCs was performed by the SPME (solid phase microextraction)/ GC-MS (gas chromatography- mass spectrometry) method. Although we found several VOCs (2-ethyl-1-decanol、 2,3,6-trimethyl-octane、Isothiocyanatocyclohexane、1, 4-Methanoazulene (Longifolene)) in mouse chamber, there is no significant difference in TIC chromatograph between healthy and infected mouse. Thus we must reconsider the method for fungal infection.
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Report
(4 results)
Research Products
(16 results)
