Molecular mechanism of the infection of hepatitis B viruses

Research Project

Project/Area Number	22659090
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Virology
Research Institution	Kanazawa University
Principal Investigator	KUROKI Kazuyuki 金沢大学, がん進展制御研究所, 准教授 (20178122)
Project Period (FY)	2010 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥2,560,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥360,000) Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywords	B型肝炎ウイルス / ウイルスベクター / HBV / DHBV / 感染 / 肝細胞 / ウイルス感染 / 宿主レセプター / 宿主レセブター
Research Abstract	For the study of entry pathway of hepatitis B viruses, we have constructed novel hepatitis B virus vectors and attempted to identify the host molecules related to the entry pathway and to establish in vitro infection systems for these viruses. As the results, we could construct novel hepatitis B virus vectors containing secretion-type luciferases as reporter genes. Unfortunately, we could not identify the host molecules relate to DHBV infection, but we were able to find human hepatoma cell HuH7 as HBV susceptible cells under the culture condition containing 2% DMSO.