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A strategy for the identification of HBV receptors with HBV pseudotype

Research Project

Project/Area Number 22659091
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionOsaka University

Principal Investigator

UEDA Keiji  大阪大学, 大学院・医学系研究科, 教授 (00221797)

Co-Investigator(Kenkyū-buntansha) NAKANO Kazushi  大阪大学, 大学院・医学系研究科, 助教 (00521725)
OHSAKI Eriko  大阪大学, 大学院・医学系研究科, 助教 (50447801)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,060,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsB型肝炎ウイルス / pseudotype / 感染受容体 / HBV感染受容体 / HBV感染システム
Research Abstract

It is near a half century since hepatitis B virus(HBV) was identified. An iceberg of HBV receptor has not been elucidated, though there are some reports on infection systems and on the receptor molecules. Thus, we still have not reached finding a real HBV receptor and there have been no useful and convenient infection system in vitro and in vivo for HBV, which makes it impossible for us to understand a precise HBV life cycle and HBV involved related diseases. Here, we designed and tried to generate an HBV pseudotype, which had a viral particle containing a retrovirus capsid and a genome inside surrounded by HBV membrane proteins. We proved successful generation of this pseudotype by immunoprecipitation with anti-HBVs antibodies and by CsCl density gradient ultracentrifugation, followed by RT-PCR targeting a retroviral gene, a GFP gene in this case, respectively. Though our established system is constructed on growth dependent integration of retroviral genomes and thus was very hard to observe its infection in a primary human hepatocytes culture system, successful generation of the HBV pseudotype will make it possible for us to perform a biological assay to clone an HBV receptor based on infectivity and will facilitate its separation and identification.
We have not identified an HBV receptor candidate yet. It has been suggested, however, that there could be an attachment factor in ordinary cultured cell lines derived from hepatocellular carcinomas.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010

All Journal Article (15 results) (of which Peer Reviewed: 9 results) Presentation (6 results) Book (1 results)

  • [Journal Article] Kaposi' s sarcoma-associated Virus governs gene expression profiles toward B cell transformation2012

    • Author(s)
      Ueda, K., Ito, E., Karayama, M., Ohsaki, E., Nakano, K., and Watanabe, S
    • Journal Title

      In-Tech" Herpesviruses", Magel, D. G. and Tyring, S., ed.

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Kaposi' s Sarcoma-Associated Herpesvirus Genome Replication, Partition and Maintenance in the Latency.2012

    • Author(s)
      Ohsaki, E. and Ueda, K.
    • Journal Title

      Frontiers in Virology

      Volume: (in press.) Pages: 1-12

    • DOI

      10.3389/fmicb.2012.00007

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of HGF attenuates the degeneration of Purkinje cell sand Bergmann glia in a knockin mouse model of spinocerebellar ataxia type 72012

    • Author(s)
      Noma S, Ohya-Shimada W, Kanai M, Ueda K, Nakamura T, Funakoshi H.
    • Journal Title

      Neurosci. Res

      Volume: 73 Issue: 2 Pages: 115-121

    • DOI

      10.1016/j.neures.2012.03.001

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] HHV-8「病原細菌・ウイルス図鑑」新居志郎ら編2012

    • Author(s)
      上田啓次
    • Journal Title

      北海道大学出版会(編集中)

      Volume: (編集中)

    • Related Report
      2011 Final Research Report
  • [Journal Article] B型肝炎のウイルス学2012

    • Author(s)
      上田啓次
    • Journal Title

      化学療法の領域

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Novel Monoclonal Antibodies for Identification of Multicentric Castleman' s Disease ; Kaposi' s Sarcoma-Associated Herpesvirus-Encoded vMIP-I and vMIP-II.2012

    • Author(s)
      Nakano, K., Katano, H., Tadagaki, K., Sato, Y., Ohsaki, E., Mori, Y., Yamanishi, K., and Ueda, K.
    • Journal Title

      Virology

      Volume: 425 Issue: 2 Pages: 95-102

    • DOI

      10.1016/j.virol.2012.01.008

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Kaposi' s sarcoma-associated virus-associated lymphomas by DNA array analysis2011

    • Author(s)
      Ueda, K., Ohsaki, E., Nakano, K., and Zheng, X.
    • Journal Title

      Leukemia Research and Diagnosis in the Era of High-throughput Genome Analysis(LRD)

      Volume: (in press) Pages: 1-12

    • DOI

      10.4061/2011/726964

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] KSHV-infected PEL cell lines exhibit a distinct gene expression profile2010

    • Author(s)
      Ueda, K., Ito, E., Karayama, M., Ohsaki, E., Nakano, K., Watanabe, S.
    • Journal Title

      Biochem. Biophsys. Res. Comm.

      Volume: 394 Pages: 482-487

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Kaposi' s sarcoma-associated herpesvirus-encoded LANA positively affects on ubiquitylation of p532010

    • Author(s)
      Suzuki, T., Isobe, T., Kitagawa, M., and Ueda, K.
    • Journal Title

      Biochem. Biophsys. Res. Comm

      Volume: 403 Pages: 194-197

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] ヘルペスウイルス学のエッセンス2010

    • Author(s)
      上田啓次
    • Journal Title

      化学療法の領域

      Volume: 26 Pages: 265-270

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Journal Article] STEALTHING, PERSISTINGウイルスの謎「潜伏感染と再活性化」2010

    • Author(s)
      上田啓次
    • Journal Title

      化学療法の領域

      Volume: 26 Pages: 1178-1179

    • Related Report
      2011 Final Research Report
  • [Journal Article] カポジ肉腫関連ヘルペスウイルス(KSHV)の潜伏感染、再活性化と病態2010

    • Author(s)
      上田啓次
    • Journal Title

      化学療法の領域

      Volume: 26 Pages: 1218-1226

    • Related Report
      2011 Final Research Report
  • [Journal Article] B型肝炎ウイルスレセプターの謎2010

    • Author(s)
      上田啓次
    • Journal Title

      Hepatoday

      Volume: No.23 Pages: 12-12

    • Related Report
      2011 Final Research Report
  • [Journal Article] Kaposi's sarcoma-associated herpesvirus-encoded LANA positively affects on ubiquitylation of p53.2010

    • Author(s)
      Suzuki, T., Isobe, T., Kitagawa, M., Ueda, K.
    • Journal Title

      Biochem.Biophsys.Res.Comm.

      Volume: 403 Pages: 194-197

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] KSHV-infected PEL cell lines exhibit a distinct gene expression profile.2010

    • Author(s)
      Ueda, K., Ito, E., Karayama, M., Ohsaki, E., Nakano, K., Watanabe, S.
    • Journal Title

      Biochem.Biophsys.Res.Comm.

      Volume: 394 Pages: 482-487

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] カポジ肉腫関連ヘルペスウイルス(KSHV)潜伏感染における潜伏感染特異的遺伝子の発現機構の解析2010

    • Author(s)
      上田啓次、大崎恵理子、中野和司
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] HIF-2αによるカポジ肉腫関連ヘルペスウイルス(KSHV)がコードするviral interferon regulatory factor 3(vIRF-3)の発現制御機構の解析2010

    • Author(s)
      中野和司、大崎恵理子、上田啓次
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] KSHVゲノムの複製・分配に関与するLANAの細胞内局在動態解析2010

    • Author(s)
      大崎恵理子、中野和司、上田啓次
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] 培養細胞におけるB型肝炎ウイルスの感染能2010

    • Author(s)
      上田啓次、大崎恵理子、中野和司
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      あわぎんホール、徳島
    • Related Report
      2011 Final Research Report
  • [Presentation] KSHV LANAの細胞内局在と複製・分配メカニズムとの関連動態解析2010

    • Author(s)
      大崎恵理子、中野和司、上田啓次
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      あわぎんホール、徳島
    • Related Report
      2011 Final Research Report
  • [Presentation] カポジ肉腫関連ヘルペスウイルスのvIRF-3/ LANA2の発現制御機構の解析2010

    • Author(s)
      中野和司、大崎恵理子、上田啓次
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      あわぎんホール、徳島
    • Related Report
      2011 Final Research Report
  • [Book] Herpesviridae-A Look into This Unique Family of Viruses2012

    • Author(s)
      Ueda, K., Ito, E., Karayama, M., Ohsaki, E., Nakano, K., Watanabe, S.
    • Publisher
      In-Tech(in press)
    • Related Report
      2011 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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