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Roles of AIRE in the development of early embryos and in the establishment of immunological self-tolerance

Research Project

Project/Area Number 22659097
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionThe University of Tokushima

Principal Investigator

MATSUMOTO Mitsuru  徳島大学, 疾患酵素学研究センター, 教授 (60221595)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsAIRE / 自己免疫疾患 / ES細胞 / 初期胚 / ゼブラフィッシュ
Research Abstract

We sought to investigate the mechanisms underlying the AIRE expression in ES cells, hoping that the results would give us a better clue for the understanding of the tolerogenic roles of Aire in medullary thymic epithelial cells. We first confirmed Aire expression from ES cells by the flow cytometric analysis, with ES cells used for the establishment of Aire^<+/ gfp> mice. Aire expression from ES cells was also confirmed from ES cells derived from Aire^<+/ gfp> mice. Interestingly, Aire/ GFP expression was observed only from a half population of the Aire^<+/ gfp> ES cells. We then sorted out GFP^+ and GFP^-cell populations, and cultured them separately for a couple of days. We found the appearance of GFP^+ cells from GFP^-cell population. We also found the appearance of GFP^-cells from GFP^+ cell population, suggesting a possible stochastic and/ or oscillating nature of Aire gene expression in ES cells.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (28 results)

All 2012 2011 2010 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (15 results) Remarks (2 results)

  • [Journal Article] Parkin interacts with Klokinl for mitochondrial import and maintenance of membrane potential2012

    • Author(s)
      Kuroda Y
    • Journal Title

      Human Molecular Genetics

      Volume: Vol.21 Issue: 5 Pages: 991-1003

    • DOI

      10.1093/hmg/ddr530

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unc93B1 restricts systemic lethal inflammation by orchestrating Toll-like receptor 7 and 9 trafficking2011

    • Author(s)
      Fukui, R., Saitoh, S.-I., Kanno, A., Onji, M., Shibata, T., Ito, A., Onji, M., Matsumoto, M., Akira, S., Yoshida, N., and Miyake, K.
    • Journal Title

      Immunity

      Volume: 35 Pages: 69-81

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lymphotoxin signal promotes thymic organogenesis by eliciting RANK expression in the embryonic thymic stroma2011

    • Author(s)
      Mouri, Y., Yano, M., Shinzawa, M., Shimo, Y., Hirota, F., Nishikawa, Y., Nii, T., Kiyonari, H., Abe, T., Uehara, H., Izumi, K., Tamada, K., Chen, L., Penninger, J. M., Inoue, J., Akiyama, T., and Matsumoto, M.
    • Journal Title

      J. Immunol

      Volume: 186 Pages: 5047-5057

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contrasting models for the roles of Aire in the differentiation program of epithelial cells in the thymic medulla2011

    • Author(s)
      Matsumoto, M.
    • Journal Title

      Eur. J. Immunol

      Volume: 41 Pages: 12-17

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aire-dependent thymic expression of desmoglein 3, the autoantigen in pemphigus vulgaris, and its role in T cell tolerance2011

    • Author(s)
      Wada, N., Nishifuji, K., Yamada, T., Kudoh, J., Shimizu, N., Matsumoto, M., Peltonen, L., Nagafuchi, S., and Amagai, M.
    • Journal Title

      J. Invest. Dermatol

      Volume: 131 Pages: 410-417

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lymphotoxin signal promotes thymic organogenesis by eliciting RANK expression in the embryonic thymic stroma2011

    • Author(s)
      Mouri Y
    • Journal Title

      Journal of Immunology

      Volume: 186 Issue: 9 Pages: 5047-5057

    • DOI

      10.4049/jimmunol.1003533

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unc93B1 restricts systemic lethal inflammation by orchestrating Toll-like receptor 7 and 9 trafficking2011

    • Author(s)
      Fukui R
    • Journal Title

      Immunity

      Volume: 35 Issue: 1 Pages: 69-81

    • DOI

      10.1016/j.immuni.2011.05.010

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contrasting models for the roles of Aire in the differentiation program of epithelial cells in the thymic medulla2011

    • Author(s)
      Matsumoto, M.
    • Journal Title

      Eur.J.Immunol.

      Volume: Vol.41 Pages: 12-17

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aire-dependent thymic expression of desmoglein 3, the autoantigen in pemphigus vulgaris, and its role in T cell tolerance2011

    • Author(s)
      Wada, N., et al.
    • Journal Title

      J.Invest.Dermatol.

      Volume: Vol.131 Pages: 410-417

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biphasic Aire expression in early embryos and in medullary thymic epithelial cells before end-stage terminal differentiation2010

    • Author(s)
      Nishikawa, Y., Hirota, F., Yano, M., Kitajima, H., Miyazaki, J-i., Kawamoto, H., Mouri, Y., and Matsumoto, M.
    • Journal Title

      J. Exp. Med

      Volume: 207 Pages: 963-971

    • NAID

      120004898443

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biphasic Aire expression in early embryos and in medullary thymic epithelial cells prior to end-stage terminal differentiation2010

    • Author(s)
      Nishikawa, Y., et al.
    • Journal Title

      J.Exp.Med.

      Volume: Vol.207 Pages: 963-971

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Expression of Aire, a gene responsible for autoimmune disease, in ES cells2011

    • Author(s)
      Matsumoto, M., et al
    • Organizer
      Cell Symposia : Stem Cell Programming & Reprogramming
    • Place of Presentation
      Lisbon, Portugal
    • Year and Date
      2011-12-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] AIRE遺伝子を導入したHeLa細胞における細胞周期依存的なAIREタンパク質のリン酸化2011

    • Author(s)
      西嶋仁、毛利安宏、西川裕美子、松本満
    • Organizer
      第40回日本免疫学会総会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Year and Date
      2011-11-27
    • Related Report
      2011 Final Research Report
  • [Presentation] egative selectionにおけるAire発現細胞由来自己抗原の役割2011

    • Author(s)
      毛利安宏、西川裕美子、西嶋仁、松本満
    • Organizer
      第40回日本免疫学会総会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Year and Date
      2011-11-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Aire-dependent organization of thymic microenvironment for the establishment of self tolerance2011

    • Author(s)
      Matsumoto, M., et al
    • Organizer
      5th Asian Congress on Autoimmunity
    • Place of Presentation
      Suntec City, Singapore
    • Year and Date
      2011-11-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] Role of self-antigen expressed by Aire-expressing cells in the negative selection process2011

    • Author(s)
      Matsumoto, M., et al
    • Organizer
      5th International Conference on Autoimmunity : Mechanisms and Novel Treatments
    • Place of Presentation
      Crete, Greece
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Aire-dependent organization of thymic microenvironment for the establishment of self tolerance2011

    • Author(s)
      Matsumoto, M., et al
    • Organizer
      EUThyme-Rolduc Meeting
    • Place of Presentation
      Leeuwenhorst, The Netherlands
    • Year and Date
      2011-05-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Role of Aire in the organization of thymic microenvironment2011

    • Author(s)
      Matsumoto, M., et al.
    • Organizer
      Controversies in Rheumatology and Autoimmunity (CORA 2011)
    • Place of Presentation
      Firenze Fiera, Florence, Italy
    • Year and Date
      2011-03-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Role of self-antigen expressed by Aire-expressing cells in the negative selection process2011

    • Author(s)
      Matsumoto, M., Mouri, Y., Nishikawa, Y., and Nishijima, H
    • Organizer
      5th International Conference on Autoimmunity : Mechanisms and Novel Treatments
    • Place of Presentation
      Knossos Royal Village Conference Center, Hersonissos, Crete, Greece
    • Related Report
      2011 Final Research Report
  • [Presentation] Aire-dependent organization of thymic microenvironment for the establishment of self tolerance2011

    • Author(s)
      Matsumoto, M., Nishikawa, Y., Mouri, Y., and Nishijima, H
    • Organizer
      EUThyme-Rolduc Meeting
    • Place of Presentation
      NH Conference Center, Leeuwenhorst, The Netherlands
    • Related Report
      2011 Final Research Report
  • [Presentation] Lymphotoxin signal promotes thymic organogenesis by eliciting RANK expression in the embryonic thymic stroma2011

    • Author(s)
      Mouri, Y., Yano, M., Shinzawa, M., Shimo, Y., Nishikawa, Y., Tamada, K., Chen, L., Penninger, J. M., Inoue, J., Akiyama, T., and Matsumoto, M.
    • Organizer
      13^<th> International TNF Conference, TNF 2011
    • Place of Presentation
      Awaji Yumebutai International Conference Center, Hyogo, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Aire-dependent organization of thymic microenvironment for the establishment of self-tolerance2011

    • Author(s)
      Nishikawa, Y., Mouri, Y., Nishijima, H., and Matsumoto, M.
    • Organizer
      ESF-JSPS Frontier Science Conference Series for Young Researchers「Cutting Edge Immunology and its Clinical Application」
    • Place of Presentation
      Apollo Hotel de Beyaerd, Hulshort, The Netherlands
    • Related Report
      2011 Final Research Report
  • [Presentation] Roles of thymic epithelial cells for the establishment of self-tolerance2010

    • Author(s)
      Matsumoto, M.
    • Organizer
      14^<th> International Congress of Immunology
    • Place of Presentation
      Kobe Portopia Hotel, Kobe, Japan
    • Year and Date
      2010-08-26
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Aire in thymic epithelial cells in the medulla controls developmental process of thymocytes2010

    • Author(s)
      Mouri, Y., Nishikawa, Y., and Matsumoto, M.
    • Organizer
      14^<th> International Congress of Immunology
    • Place of Presentation
      Kobe Portopia Hotel, Kobe, Japan
    • Year and Date
      2010-08-24
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Biphasic Aire expression in early embryos and in medullary thymic epithelial cells prior to end-stage terminal differentiation2010

    • Author(s)
      Nishikawa, Y., Mouri, Y., and Matsumoto, M.
    • Organizer
      14^<th> International Congress of Immunology
    • Place of Presentation
      Kobe Portopia Hotel, Kobe, Japan
    • Year and Date
      2010-08-23
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] AIRE and autoimmune disease2010

    • Author(s)
      Matsumoto, M., Nishikawa, Y., Hirota, F. and Mouri, Y.
    • Organizer
      The 19^<th> International Rheumatology Symposium
    • Place of Presentation
      Kobe Portopia Hotel, Kobe, Japan
    • Year and Date
      2010-04-23
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.tokushima-u.ac.jp/ier/autoimmunity/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://mms1.ier.fokushima-u.ac.jp/article/0017775.html

    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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