| Project/Area Number |
22659110
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied pharmacology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HASHIDA Yasuhiko 京都大学, 物質-細胞統合システム拠点, 研究員 (30512462)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,070,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | 遺伝子治療 / 遺伝子導入 / 遺伝子デリバリー / 肝臓 / 腎臓 / 長期発現 / 長期遺伝子発現系 / phiC31インテグラーゼ / 組織核酸導入法 / 遺伝子機能解析 / in vivo長期遺伝子発現 / 組織押圧核酸導入法 |
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| Research Abstract |
In vivo gene transfection technology is an important method for the establishment of gene therapy, gene functional analysis, and diseased animal model. In this study, we have succeeded to develop a long-term gene transfection method that is induced by integration of specific sites in the genome and tissue pressure and/or suction-mediated gene transfection methods for naked plasmid DNA. This information would be efficient for the establishment of new therapeutic strategies for refractory diseases.
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