A new method for stress assessment using stress-specific alternative splice variants

• Project/Area Number: 22659142
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: The University of Tokushima
• Principal Investigator: ROKUTAN Kazuhito 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10230898)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,190,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: ストレス / ストレス関連疾患 / バイオマーカー / 選択的スプライシング / スプライシングバリアント / マイクロRNA

Research Abstract

Alternative splicing events in healthy medical students challenging an examination for promotion were analyzed using exon array and real-time RT-PCR. We identified the stress-initiated skipping of exon 63 of SMG-1 that encodes a phosphatidylinositol 3-kinase-related protein kinase crucial for activations of p53-dependent pathways(Neurosci Lett 2010). Using National Medical License Examination as a chronic psychological stress model, we also examined alternative splicing of the glucocorticoid receptor(GR) gene in medical students, and found that the persistant stressful situations specifically decreased a non-functional isoform(GR-beta) in medical students(Stress 2011).

Research Products

• [Journal Article] Psychological Resources as Mediators of the Association Between Social Class and Health: Comparative Findings from Japan and the USA. (2012)
  • Author(s): Kan C, Kawakami N, Karasawa M, Love GD, Coe CL, Miyamoto Y, Ryff CD, Kitayama S, Curhan KB, Markus HR.
  • Journal Title: Int J Behav Med. Volume: 49(7) Issue: 7 Pages: 991-7
  • DOI: 10.1111/j.1469-8986.2012.01371.x
  • Peer Reviewed
• [Journal Article] MicroRNAs miR-144/144* and miR-16 in peripheral blood are potential biomarkers for naturalistic stress in healthy Japanese medical students. (2012)
  • Author(s): Katsuura S, Kuwano Y, Yamagishi N, Kurokawa K, Kajita K, Akaike Y, Nishida K, Masuda K, Tanahashi T, Rokutan K.
  • Journal Title: Neurosci Lett. Volume: 516 Issue: 1 Pages: 79-84
  • DOI: 10.1016/j.neulet.2012.03.062
  • Peer Reviewed
• [Journal Article] Role of miR-19b and its target mRNAs in 5-fluorouracil resistance in colon cancer cells (2012)
  • Author(s): Kurokawa K, Tanahashi T, Iima T, Yamamoto Y, Akaike Y, Nishida K, Masuda K, Kuwano Y, Murakami Y, Fukushima M, Rokutan
  • Journal Title: J Gastroenterol Volume: 47 Issue: 8 Pages: 883-895
  • DOI: 10.1007/s00535-012-0547-6
  • NAID: 10031051201
  • Peer Reviewed
• [Journal Article] MicroRNAs miR-144/144^* and miR-16 in peripheral blood are potential biomarkers for naturalistic stress in healthy Japanese medical students (2012)
  • Author(s): Katsuura S, et al
  • Journal Title: Neuroscience Letters Volume: 516 Pages: 79-84
  • Peer Reviewed
• [Journal Article] Circulating cytokine signatures in healthy medical students exposed to academic examination stress (2012)
  • Author(s): Kamezaki Y, et al
  • Journal Title: Psychophysiology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Role of miR-19b and its target mRNAs in 5-fluorouracil resistance in colon cancer cells (2012)
  • Author(s): Kurokawa K, et al
  • Journal Title: Journal of Gastroenterology Volume: (In press)
  • Peer Reviewed
• [Journal Article] General RBP expression in human tissues as a function of age (2012)
  • Author(s): Masuda K, et al
  • Journal Title: Aging Research Review Volume: (In press)
  • Peer Reviewed
• [Journal Article] Autism-associated gene expression in peripheral leucocytes commonly observed between subjects with autism and healthy women having autistic children. (2011)
  • Author(s): Kuwano Y, Kamio Y, Kawai T, Katsuura S, Inada N, Takaki A, Rokutan K.
  • Journal Title: PLoS One Volume: 6 Issue: 9 Pages: e24723-e24723
  • DOI: 10.1371/journal.pone.0024723
  • Peer Reviewed
• [Journal Article] Skipping of an alternative intron in the srsf1 3' untranslated region increases transcript stability (2011)
  • Author(s): Akaike Y, Kurokawa K, Kajita K, Kuwano Y, Masuda K, Nishida K, Kang SW, Tanahashi T, Rokutan K
  • Journal Title: The Journal of Medical Investigation Volume: 58 Issue: 3,4 Pages: 180-187
  • DOI: 10.2152/jmi.58.180
  • NAID: 130004822655
  • ISSN: 1343-1420, 1349-6867
  • Peer Reviewed
• [Journal Article] Effects of chronic academic stress on mental state and expression of glucocorticoid receptor α and β isoforms in healthy Japanese medical students. (2011)
  • Author(s): Kurokawa K, Tanahashi T, Murata A, Akaike Y, Katsuura S, Nishida K, Masuda K, Kuwano Y, Kawai T, Rokutan K.
  • Journal Title: Stress Volume: 14 Issue: 4 Pages: 431-438
  • DOI: 10.3109/10253890.2011.555930
  • Peer Reviewed
• [Journal Article] Circulating vascular endothelial growth factor is independently and negatively associated with trait anxiety and depressive mood in healthy Japanese university students. (2011)
  • Author(s): Katsuura S, Kamezaki Y, Yamagishi N, Kuwano Y, Nishida K, Masuda K, Tanahashi T, Kawai T, Arisawa K, Rokutan K.
  • Journal Title: Int J Psychophysiol Volume: 81 Issue: 1 Pages: 38-43
  • DOI: 10.1016/j.ijpsycho.2011.04.004
  • Peer Reviewed
• [Journal Article] Skipping of an alternative intron in the srsf1 3' untranslated region increases transcript stability (2011)
  • Author(s): Akaike Y, et al
  • Journal Title: Journal of Medical Investigation Volume: 58 Pages: 180-187
  • Peer Reviewed
• [Journal Article] Circulating vascular endothelial growth factor is independently and negatively associated with trait anxiety and depressive mood in healthy Japanese university students (2011)
  • Author(s): Katsuura S, et al
  • Journal Title: International Journal of Psychophysiology Volume: 81 Pages: 38-43
  • Peer Reviewed
• [Journal Article] Effects of chronic academic stress on mental state and expression of glucocorticoid receptor α and β isoforms in healthy Japanese medical students (2011)
  • Author(s): Kurokawa K, et al
  • Journal Title: Stress Volume: 14 Pages: 431-438
  • Peer Reviewed
• [Journal Article] Effects of chronic academic stress on mental state and expression of glucocorticoid receptor α and β isoforms in healthy Japanese medical students (2011)
  • Author(s): Ken Kurokawa, et al.
  • Journal Title: Stress Volume: (in press)
  • Peer Reviewed
• [Journal Article] Interleukin-18 deficiency reduces neuropeptide gene expressions in the mouse amygdala related with behavioral chang (2010)
  • Author(s): Yamamoto Y, Tanahashi T, Katsuura S, Kurokawa K, Nishida K, Kuwano Y, Kawai T, Teshima-Kondo S, Chikahisa S, Tsuruo Y, Sei H, Rokutan K.
  • Journal Title: J Neuroimmunol Volume: 229 Issue: 1-2 Pages: 129-139
  • DOI: 10.1016/j.jneuroim.2010.07.024
  • Peer Reviewed
• [Journal Article] Brief naturalistic stress induces an alternative splice variant of SMG-1 lacking exon 63 in peripheral leukocyte (2010)
  • Author(s): Kurokawa K, Kuwano Y, Tominaga K, Kawai T, Katsuura S, Yamagishi N, Satake Y, Kajita K, Tanahashi T, Rokutan K
  • Journal Title: Neurosci Lett Volume: 484 Issue: 2 Pages: 128-132
  • DOI: 10.1016/j.neulet.2010.08.031
  • Peer Reviewed
• [Journal Article] High-throughput screening of brief naturalistic stress-responsive cytokines in university students taking examinations (2010)
  • Author(s): Katsuura S, Kamezaki Y, Tominaga K, Masuda K, Nishida K, Yamamoto Y, Takeo K, Yamagishi N, Tanahashi T, Kawai T, Rokutan K
  • Journal Title: Int J Psychophysiol Volume: 77 Issue: 2 Pages: 135-140
  • DOI: 10.1016/j.ijpsycho.2010.05.004
  • Peer Reviewed
• [Journal Article] Brief naturalistic stress induces an alternative splice variant of SMG-1 lacking exon 63 in peripheral leukocytes (2010)
  • Author(s): Ken Kurokawa, et al.
  • Journal Title: Neuroscience Letters Volume: 484 Pages: 128-132
  • Peer Reviewed
• [Journal Article] Interleukin-18 deficiency reduces neuropeptide gene expressions in the mouse amygdala related with behavioral change (2010)
  • Author(s): Yuta Yamamoto, et al.
  • Journal Title: Journal of Neuroimmunology Volume: 229 Pages: 129-139
  • Peer Reviewed
• [Journal Article] High-throughput screening of brief naturalistic stress-responsive cytokines in university students taking examinations (2010)
  • Author(s): Sakurako Katsuura, et al.
  • Journal Title: International Journal of Psychophysiology Volume: 77 Pages: 135-140
  • Peer Reviewed
• [Presentation] Autism-associated gene expression signatures in peripheral blood leucocytes (2011)
  • Author(s): 桑野由紀、神尾陽子、河合智子、勝浦桜子、稲田尚子、高木晶子、六反一仁
  • Organizer: Joint Academic Conference on ASD 2011
  • Place of Presentation: 日本財団ビル(東京都)
  • Year and Date: 2011-12-02
• [Presentation] 精神的ストレスに応答するヒト末梢血マイクロRNAの発現 (2011)
  • Author(s): 本田真奈美、桑野由紀、山岸直子、黒川憲、佐竹譲、梶田敬介、赤池瑶子、西田憲生、増田清士、棚橋俊仁、六反一仁
  • Organizer: 第27回日本ストレス学会学術総会
  • Place of Presentation: 東京国際交流館(東京都)
  • Year and Date: 2011-11-20
• [Presentation] 自閉症スペクトラム成人と自閉症児の母親に共通する末梢白血球の遺伝子発現の特徴 (2011)
  • Author(s): 六反一仁、桑野由紀、神尾陽子
  • Organizer: 第107回日本精神神経学会学術総会シンポジウム
  • Place of Presentation: ホテルグランパシフィックLE DAIBA(東京都)
  • Year and Date: 2011-10-27
• [Presentation] High-throughput screening of immunomodulators identifies VEGF as a potential biomarker for trait anxiety and depressive mood in healthy Japanese university students (2011)
  • Author(s): Honda M, Katsuura S, Kuwano Y, Yamagishi N, Kurokawa K, Satake Y, Kajita K, Akaike Y, Nishida K, Masuda K, Tanahashi T, Rokutan K
  • Organizer: The International Conference on Social Stratification and Health 2011
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2011-08-06
• [Presentation] 遺伝子発現解析からみた慢性疲労症候群の病態 (2011)
  • Author(s): 六反一仁
  • Organizer: 第7回日本疲労学会総会・学術集会
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2011-05-21
• [Presentation] 遺伝子発現解析からみた慢性疲労症候群の病態 (2011)
  • Author(s): 六反一仁
  • Organizer: 第7回日本疲労学会総会
  • Place of Presentation: 名古屋大学(名古屋市)(招待講演)
  • Year and Date: 2011-05-21
• [Presentation] 酸化ストレス下での、HuRによるtransformer 2-beta(Tra2-beta)の選択的スプライシング制御機構 (2011)
  • Author(s): 赤池瑶子、増田清士、山岸直子、黒川憲、佐竹譲、梶田敬介、本田真奈美、西田憲生、桑野由紀、六反一仁
  • Organizer: 第6回臨床ストレス応答学会大会
  • Place of Presentation: 名古屋大学(名古屋市)
• [Presentation] ストレスマーカ-の現状 (2010)
  • Author(s): 六反一仁
  • Organizer: 第26回日本ストレス学会学術総会
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2010-11-06
• [Presentation] ストレスマーカーの現状 (2010)
  • Author(s): 六反一仁
  • Organizer: 第26回日本ストレス学会学術総会教育講演
  • Place of Presentation: 福岡市
  • Year and Date: 2010-11-06
• [Presentation] 心と遺伝子を探るストレスゲノミクス研究 (2010)
  • Author(s): 六反一仁
  • Organizer: 2010日本歯周病学会秋期学術大会
  • Place of Presentation: かがわ国際会議場(高松市)
  • Year and Date: 2010-09-19
• [Presentation] 心と遺伝子を探るストレスゲノミクス研究 (2010)
  • Author(s): 六反一仁
  • Organizer: 2010日本歯周病学会秋期学術大会(第53回)特別講演
  • Place of Presentation: 高松市
  • Year and Date: 2010-09-19
• [Presentation] Alternative splice variants of SMG-1 as a potential marker for brief naturalistic stressors in peripheral leukocytes (2010)
  • Author(s): Yuki Kuwano, Ken Kurokawa, Sakurako Katsuura, Naoko Yamagishi, Yuzuru Satake, Keisuke Kajita, Toshihito Tanahashi, Kazuhito Rokutan
  • Organizer: The 7^<th> World Congress on Stress
  • Place of Presentation: Leiden University(Leiden), Netherlands
• [Presentation] High-throughput screening of brief naturalistic stressresponsive cytokines in Japanese university students taking examinations (2010)
  • Author(s): Sakurako Katsuura, Yoshiko Kamezaki, Yuki Kuwnao, Naoko Yamagishi, Ken Kurokawa, Yuzuru Satake, Toshihito Tanahashi, Kazuhito Rokutan
  • Organizer: The 7^<th> World Congress on Stress
  • Place of Presentation: Leiden University(Leiden), Netherlands
• [Presentation] High-throughput screening of immunomodulators identifies VEGF as a potential biomarker for trait anxiety and depressive mood in healthy Japanese university students (2010)
  • Author(s): Sakurako Katsuura, Yoshiko Kamezaki, Yuki Kuwnao, Naoko Yamagishi, Ken Kurokawa, Keisuke Kajita, Toshihito Tanahashi, Kazuhito Rokutan
  • Organizer: The 7^<th> World Congress on Stress
  • Place of Presentation: Leiden University(Leiden), Netherlands
• [Presentation] Alternative splice variants of SMG-1 as a potential marker for brief naturalistic stressors in peripheral leukocytes (2010)
  • Author(s): Kazuhito Rokutan
  • Organizer: The 7^<th> World Congress on Stress
  • Place of Presentation: Leiden, Netherlands
• [Presentation] High-throughput screening of brief naturalistic stress-responsive cytokines in Japanese university students taking examinations (2010)
  • Author(s): Kazuhito Rokutan
  • Organizer: The 7^<th> World Congress on Stress
  • Place of Presentation: Leiden, Netherlands
• [Presentation] High-throughput screening of immunomodulators identifies VEGF as a potential biomarker for trait anxiety and depressive mood in healthy Japanese university students (2010)
  • Author(s): Kazuhito Rokutan
  • Organizer: The 7^<th> World Congress on Stress
  • Place of Presentation: Leiden, Netherlands
• [Presentation] The mechanism of transfomer 2-□ gene expression in response to oxidative stress in human colon epithelial cells (2010)
  • Author(s): Kazuhito Rokutan
  • Organizer: Digestive Disease Week 2010
  • Place of Presentation: New Orleans, USA