| Project/Area Number |
22659142
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | The University of Tokushima |
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Principal Investigator |
ROKUTAN Kazuhito 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10230898)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,190,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | ストレス / ストレス関連疾患 / バイオマーカー / 選択的スプライシング / スプライシングバリアント / マイクロRNA |
|---|
| Research Abstract |
Alternative splicing events in healthy medical students challenging an examination for promotion were analyzed using exon array and real-time RT-PCR. We identified the stress-initiated skipping of exon 63 of SMG-1 that encodes a phosphatidylinositol 3-kinase-related protein kinase crucial for activations of p53-dependent pathways(Neurosci Lett 2010). Using National Medical License Examination as a chronic psychological stress model, we also examined alternative splicing of the glucocorticoid receptor(GR) gene in medical students, and found that the persistant stressful situations specifically decreased a non-functional isoform(GR-beta) in medical students(Stress 2011).
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