Identification of alveolar cell induction genes and differentiation of iPS cells into alveolar cells

• Project/Area Number: 22659160
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Hirosaki University
• Principal Investigator: SAIJYO Yasuo 弘前大学, 大学院・医学研究科, 教授 (10270828)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,060,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 非閉塞性肺疾患癌 / 肺線維症 / 呼吸器感染症 / その他 / iPS細胞 / 肺細胞II型細胞 / 分化 / 肺幹細胞 / 肺胞上皮細胞

Research Abstract

In this study, we tried to differentiate mouse iPS cells to alveolar cells in vitro. At first, iPS cells were cultured with ActinA and Wnt3a for 7 days. iPS cells differentiated into endodermal cells expressing Sox17 and Foxa2. iPS cells were further cultured with FGF2 for additional 5 days. Finally, about 8 % iPS cells differentiated into alveolar type II cells determined by SP-C expression and electron microscopy.

Research Products

• [Journal Article] Paracrine Factors of Multipotent Stromal Cells Ameliorate Lung Injury in an Elastase-induced Emphysema Model (2011)
  • Author(s): Ahmad Katsha, Shinya Ohkouchi, Toshihiro Nukiwa, Yasuo Saijo
  • Journal Title: Molecular Therapy Volume: 19 Issue: 1 Pages: 196-203
  • DOI: 10.1038/mt.2010.192
  • Peer Reviewed
• [Journal Article] Mesenchymal Stromal Cells Promote Tumor Growth Through the Enhancement of Neovascularization (2011)
  • Author(s): Suzuki K, Saijo Y, at all
  • Journal Title: Molecular Medicine Volume: Vul.17 Issue: 7-8 Pages: 579-587
  • DOI: 10.2119/molmed.2010.00157
  • Peer Reviewed
• [Journal Article] Low-dose gefitinib treatment for patients with advanced non-small cell lung cancer harboring sensitive epidermal growth factor receptor mutations (2011)
  • Author(s): Satoh H, Inoue A, Kobayashi K, Maemondo M, Oizumi S, Isobe H, Gemma A, Saijo Y, Yoshizawa H, Hagiwara K, Nukiwa T
  • Journal Title: J Thorac Oncol Volume: 6(8) Issue: 8 Pages: 1413-7
  • DOI: 10.1097/jto.0b013e31821d43a8
  • Peer Reviewed
• [Journal Article] Paracrine factors from Mesenchymal Stem Cells ameliorate lung injuries in elastase induced emphysema. (2011)
  • Author(s): Katsha AM, Saijo Y, et al.
  • Journal Title: Molecular Therapy Volume: 19 Pages: 196-203
  • Peer Reviewed
• [Journal Article] Randomized phase II trial of weekly paclitaxel combined with carboplatin versus standard paclitaxel combined with carboplatin for elderly patients with advanced non-small-cell lung cancer (2010)
  • Author(s): Sakakibara T, Inoue A, Saijo Y, at all
  • Journal Title: Annals of Oncology Volume: Vol.21,No.4 Issue: 4 Pages: 795-799
  • DOI: 10.1093/annonc/mdp401
  • Peer Reviewed
• [Journal Article] North-East Japan Study Group. Gefitinib or chemotherapy for non-small-cell lung cancer with mutated EGFR (2010)
  • Author(s): Maemondo M, Inoue A, Kobayashi K, Sugawara S, Oizumi S, Isobe H, Gemma A, Harada M, Yoshizawa H, Kinoshita I, Fujita Y, Okinaga S, Hirano H, Yoshimori K, Harada T, Ogura T, Ando M, Miyazawa H, Tanaka T, Saijo Y, Hagiwara K, Morita S, Nukiwa T
  • Journal Title: N Engl J Med Volume: 362(25) Issue: 25 Pages: 2380-8
  • DOI: 10.1056/nejmoa0909530
  • Peer Reviewed
• [Journal Article] Gefitinib or Chemotherapy for Non-Small Cell Lung Cancer with Mutated EGFR. (2010)
  • Author(s): Maemondo M, Saijo Y, et al.
  • Journal Title: New England of Journal of Medicine Volume: 362 Pages: 2380-2388
  • Peer Reviewed
• [Journal Article] Randomized phase II trial of weekly paclitaxel combined with carboplatin versus standard paclitaxel combined with carboplatin for elderly patients with advanced non-small-cell lung cancer. (2010)
  • Author(s): Sakakibara T, Saijo Y, et al
  • Journal Title: Annals of Oncology Volume: 21 Pages: 795-799
  • Peer Reviewed
• [Presentation] マウスiPS細胞におけるin vitro肺上皮細胞への分化誘導 (2012)
  • Author(s): 周啓亮
  • Organizer: 第52回日本呼吸器学会学術講演会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2012-04-21
• [Presentation] マウス成長肺と傷害修復過程における肺幹細胞の動態解析 (2010)
  • Author(s): 孫若文
  • Organizer: 第50回日本呼吸器学会学術講演会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2010-04-24
• [Presentation] マウス成長肺と傷害修復通程における肺幹細胞の動態解析 (2010)
  • Author(s): 孫若文、西條康夫, ら
  • Organizer: 第50回日本呼吸器学会学術講演会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2010-04-24