| Project/Area Number |
22659168
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MIZUSAWA Hidehiro 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (30144091)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIBASHI Satoru 東京医科歯科大学, 医学部附属病院, 助教 (30533369)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,230,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | 神経分子病態学 / 脊髄小脳変性症 / ES細胞 / 骨髄間葉系幹細胞 / プルキンエ細胞 / 神経新生 / 再生医療 / モデル動物 / 脊髄小脳変成症 |
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| Research Abstract |
The purpose of this study is to establish novel stem cell therapy for spinocerebellar degeneration.
We successfully established a system that induced the maturation of Purkinje cells(PCs) derived from mouse ES cells(ESC). ESC-derived Purkinje cells(EDPCs) showed the morphology of mature PCs and synaptogenesis with other cerebellar neurons. Furthermore, the electrophysiological properties of these EDPCs were compatible with those of native mature PCs in vitro, which were assessed by whole-cell patch-clamp recordings. Next, we transplanted these EDPCs into the cerebellum of Purkinje cell degeneration(PCD) mouse. Transplanted EDPCs showed abilities to survive and maintain mature Purkinje cell morphology in vivo.
Thus, EDPCs show promise as a powerful source for stem cell therapies aimed at spinocerebellar degeneration.
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