| Project/Area Number |
22659183
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Waseda University |
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Principal Investigator |
IKEDA Yasuo 早稲田大学, 理工学術院, 教授 (00110883)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUBARA Yumiko 慶應義塾大学, 医学部, 特任講師 (70365427)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,290,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | 血栓止血学 / 再生医療 / 血小板 / 巨核球 / 骨髄脂肪変性 / 造血幹細胞 / 間葉系幹細胞 / 脂肪前駆細胞 / 分化誘導 / 細胞運命制御 / 細胞分化運命制御 |
|---|
| Research Abstract |
We carried out screening of megakaryocyte-inducing factors based on our previous findings ; megakaryocytes and platelets were differentiated from preadipocyte cell line 3T3-L1, but not its parent cell line 3T3 fibroblasts. Gene expression analysis of candidate transcription factors and gene transfection study showed that p45NF-E2, Maf G, and Maf K are determinant factors for megakaryocyte differentiation and platelet production. Megakaryocyte differentiation of p45NF-E2/Maf G/Maf K-expressing fibroblasts was inhibited by the co-expression of CEBP alpha, known to be a determinant factor for adipocytes. Thus, p45NF-E2/Maf G/Maf K and CEBPalpha are critical factors in cell-fate swiching between megakaryocytes and adipocytes.
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