Environmental influence on embryonic brain development

• Project/Area Number: 22659199
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Osaka University
• Principal Investigator: MURAKAMI Fujio 大阪大学, 生命機能研究科, 教授 (20089882)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,230,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 胎児医学 / in vivoイメージング / マウス / 電気穿孔 / FRET / 電位センサー / Ca^<2+>センサー

Research Abstract

The mechanisms that cause developmental disorders remain obscure despite its importance. In this study, we carried out live imaging of migrating cortical interneurons by using mouse embryos separated from their dams with the umbilical cord attached. We carried out imaging of migrating neurons in an attempt to evaluate the effect of environmental influence.

Research Products

• [Journal Article] Formation of Axon-dendrite Polarity in situ : Initiation of Axons from Polarized and Non-polarized, (2012)
  • Author(s): Yumiko Hatanaka, Kenta Yamauchi and Fujio Murakami
  • Journal Title: Dev Growth Differ Volume: 54巻 Pages: 398-407
  • Peer Reviewed
• [Journal Article] Intrinsic and Extrinsic Mechanisms Control the Termination of Cortical Interneuron Migration (2012)
  • Author(s): Naoko Inamura, Toshiya Kimura, Satoshi Tada, Takashi Kurahashi, Mitsutoshi Yanagida, Yuchio Yanagawa, Kazuhiro Ikenaka, and Fujio Murakami
  • Journal Title: J Neurosci Volume: 32巻 Pages: 6032-6042
  • Peer Reviewed
• [Journal Article] Formation of Axon-dendrite Polarity in situ : Initiation of Axons from Polarized and Non-polarized (2012)
  • Author(s): Y.Hatanaka, K.Yamauchi, F.Murakami
  • Journal Title: Cells Development Growth and Differentiation Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Intrinsic and Extrinsic Mechanisms Control the Termination of Cortical Interneuron Migration (2012)
  • Author(s): N.Inamura, T.Kimura, S.Tada, T.Kurakashi K, Y.Yanagida, Y.Yanagawa, K.Ikenaka, F.Murakami
  • Journal Title: J Neurosci Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Role of Neph2 in pontine nuclei formation in the developing hindbrain (2011)
  • Author(s): Kazuhiko Nishida, Kazuhide Nakayama, Saori Yoshimura and Fujio Murakami
  • Journal Title: Mol Cell Neurosci Volume: 46巻 Pages: 662-670
  • URL: http://dx.doi.org/10.1016/j.mcn.2011.01.007
  • Peer Reviewed
• [Journal Article] Role of Neph2 in pontine nuclei formation in the developing hindbrain (2011)
  • Author(s): K.Nishida, K.Nakayama, S.Yoshimura, F.Murakami
  • Journal Title: Mol Cell Neurosci Volume: 46 Issue: 3 Pages: 662-670
  • DOI: 10.1016/j.mcn.2011.01.007
  • Peer Reviewed
• [Journal Article] Temporally-and Spatially-Regulated Generation of Distinct Descendants by Sonic Hedgehog-Expressing Progenitors in the Forebrain (2010)
  • Author(s): Yukiko Wada, Kenta Yamauchi, Fujio Murakami, Yasuto Tanabe
  • Journal Title: Dev Neurobiol Volume: (オンラインでのみ公開中)
  • URL: http://onlinelibrary.wiley.com/doi/10.1002/dneu.20861/pdf
  • Peer Reviewed
• [Journal Article] Cortical GABAergic interneurons transiently assume a sea urchin-like non-polarized shape prior to axon initiation (2010)
  • Author(s): Yamasaki, E., Tanaka, DH., Yanagawa, Y., and Murakami, F
  • Journal Title: J. Neurosci Volume: 30巻 Pages: 15221-15227
  • Peer Reviewed
• [Journal Article] CXCR4 is Required for Proper Regional and Laminar Distribution of Cortical Somatostatin-, Calretinin-and Neuropeptide Y-expressing GABAergic Interneurons (2010)
  • Author(s): Daisuke H. Tanaka, Sakae Mikami, Takashi Nagasawa, Jun-ichi Miyazaki, Kazunori Nakajima and Fujio Murakami
  • Journal Title: Cerebral Cortex Volume: 20巻 Pages: 2810-2817
  • Peer Reviewed
• [Journal Article] Cortical GABAergic interneurons transiently assume a sea urchin-like nonpolarized shape before axon initiation. (2010)
  • Author(s): Emi Yamasaki, Daisuke H.Tanaka, Yuchio Yanagawa, Fujio Murakami
  • Journal Title: J Neurosci. Volume: 30 Pages: 15221-15227
  • Peer Reviewed
• [Journal Article] CXCR4 is Required for Proper Regional and Laminar Distribution of Cortical Somatostatin-, Calretinin- and Neuropeptide Y-expressing GABAergic Interneurons (2010)
  • Author(s): Daisuke H.Tanaka, Sakae Mikami, Takashi Nagasawa, Jun-ichi Miyazaki, Kazunori Nakajima, Fujio Murakami
  • Journal Title: Cerebral Cortex Volume: 20 Pages: 2810-2817
  • Peer Reviewed
• [Presentation] Migration, its Termination, Axon Initiation and Maturation of Cortical Interneurons (2012)
  • Author(s): 村上富士夫
  • Organizer: 第59回NIBB CONFERENCE" Neocortical Organization"
  • Place of Presentation: 岡崎コンファレンスセンター
• [Presentation] Migration, its Termination, Axon Initiation and Maturation of Cortical Interneurons (2012)
  • Author(s): 村上富士夫
  • Organizer: 第59回NIBB CONFERENCE "Neocortical Organization"
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県)(招待講演)
• [Presentation] 皮質介在ニューロンの移動、成熟と軸索形成 (2011)
  • Author(s): 村上富士夫
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-16
• [Presentation] 内在性要因と環境が大脳皮質抑制性ニューロンの移動の停止を調節する (2011)
  • Author(s): 稲村直子、木村俊哉、多田智史、倉橋崇、柳川右千夫、池中一裕、村上富士夫
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-16
• [Presentation] 線条体striosome/ matrix構造形成過程におけるmedium spiny neuronの発生・発達様式 (2011)
  • Author(s): 萩本和也、高見紗季、鳥越万紀夫、村上富士夫、田辺康人
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-16
• [Presentation] 皮質在介ニューロンの移動、成熟と軸索形成 (2011)
  • Author(s): 村上富士夫
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-16
• [Presentation] 内在性要因と環境が大脳皮質抑制性ニューロンの移動の停止を調節する (2011)
  • Author(s): 稲村直子, 木村俊哉, 多田智史, 倉橋崇, 柳川右千夫, 池中一裕, 村上富士夫
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-16
• [Presentation] 線条体striosome/matrix構造形成過程におけるmedium spiny neuronの発生・発達様式 (2011)
  • Author(s): 萩本和也, 高見紗季, 鳥越万紀夫, 村上富士夫, 田辺康人
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-16
• [Presentation] Achemokine receptor CXCR7 non-cell-Autonomously controls migration and nuclei formation of pontine neurons from the migratory environment (2011)
  • Author(s): Yan Zhu, Xun Hong, Koki Shirosaki, Frederic Sierro, Fabienne Mackay, Takashi Nagasawa, Fujio Murakami
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-15
• [Presentation] A chemokine receptor CXCR7 non-cell-autonomously controls migration and nuclei formation of pontine neurons from the migratory environment (2011)
  • Author(s): Yan Zhu, Xun Hong, Koki Shirosaki, Frederic Sierro, Fabienne Mackay, Takashi Nagawasa, Fujio Murakami
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-15
• [Presentation] 生体内の胎仔脳における神経細胞移動 (2010)
  • Author(s): 村上富士夫、柳田光俊
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-02
• [Presentation] 小脳ニューロンの時空間的な発生制御-子宮内電気穿航孔法を用いた解析- (2010)
  • Author(s): 喜多善亮、西田和彦、川上浩一、高橋淑子、村上富士夫
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-02
• [Presentation] 橋核形成における3次元的方向転換 (2010)
  • Author(s): 篠原正樹、Yan Zhu、村上富士夫
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-02
• [Presentation] ソニックヘッジホッグ系譜細胞の前脳における時期依存的な発生様式 (2010)
  • Author(s): 和田有希子、山内健太、村上富士夫、田辺康人
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-02
• [Presentation] 2光子in vivoリアルタイムイメージングによる大脳皮質介在ニューロンの動態観察 (2010)
  • Author(s): 柳田光俊、三好良太、豊國龍平、村上富士夫
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-02
• [Remarks]
  • URL: http://square.umin.ac.jp/murakami-lab/