• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Involvement of microflora-induced Th17 cells in psoriasis

Research Project

Project/Area Number 22659207
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionEhime University

Principal Investigator

SAYAMA Koji  愛媛大学, 大学院・医学系研究科, 教授 (80187286)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,230,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywords無菌マウス / SPF / 自然免疫 / Th17 / 黄色ブドウ球菌 / 表皮ブドウ球菌 / TLR / 細菌感染 / 尋常性乾癬 / 角化細胞 / インフラマソーム / 細菌 / 表皮分化
Research Abstract

Skin phenotypes of germ-free mice were compared with those of control SPF mice. The epidermis of germ-free mice was atrophic compared to SPF mice. Markers for keratinocyte differentiation such as keratins were histologically stained. Next, we studied whether pathogens can be a trigger for the development of psoriasis. S. aureus and S. epidermidis were heat-inactivated and added to the cultured keratinocytes. Cytokines and anti-microbial peptides were induced. Imiquimod, an activator of innate immunity, was used for the development of psoriatic skin lesions. Back skin of the mice was shaved and imiquimod was topically applied for the five consecutive days. One week after the application, erythema and scales appeared on the skin. Histological analyses confirmed the development of psoriasis.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (11 results)

All 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (3 results)

  • [Journal Article] Mite allergen is a danger signal for the skin via activation of the inflammasome in keratinocytes2011

    • Author(s)
      Dai X, Sayama K, Tohyama M, Shirakata Y, Hanakawa Y, Tokumaru S, Yang L, Hirakawa S, and Hashimoto K.
    • Journal Title

      J Allergy Clin Immunol

      Volume: 127 Pages: 806-814

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear translocation of phosphorylated STAT3 regulates VEGF-A-induced lymphatic endothelial cell migration and tube formation2011

    • Author(s)
      Okazaki H, Tokumaru S, Hanakawa Y, Shiraishi K, Shirakata Y, Dai X, Yang L, Tohyama M, Hashimoto K, and Sayama K.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 412 Pages: 441-445

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inflammatory mediator TAK1 regulates hair follicle morphogenesis and anagen induction shown by using keratinocyte-specific TAK1-dificient mice.2010

    • Author(s)
      Sayama K, Kajiya K, Sugawara K, Sato S, Hirakawa S, Shirakata Y, Hanakawa Y, Dai X, Ishimatsu-Tsuji Y, Metzger D, Chambon P, Akira S, Paus R, Kishimoto J, and Hashimoto K.
    • Journal Title

      PLoS One

      Volume: 5

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] E2 polyubiquitin-conjugating enzyme Ubc 13 in keratinocytes is essential for epidermal integrity2010

    • Author(s)
      Sayama K, Yamamoto M, Shirakata Y, Hanakawa Y, Hirakawa S, Dai X, Tohyama M, Tokumaru S, Shin MS, Sakurai H, Akira S, and Hashimoto K.
    • Journal Title

      J Biol Chem

      Volume: 285 Pages: 30042-30049

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] PPARγmediates innate immunity by regulating the 1α, 25-dihydroxyvitamin D3 induced hBD-3 and cathelicidin in human keratinocytes2010

    • Author(s)
      Dai X, Sayama K, Tohyama M, Shirakata Y, Hanakawa Y, Tokumaru S, Yang L, Hirakawa S, and Hashimoto K.
    • Journal Title

      J Dermatol Sci

      Volume: 60 Pages: 179-186

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] E2 polyubiquitin-conjugating enzyme Ubc13 in keratinocytes is essential for epidermal integrity.2010

    • Author(s)
      Sayama K
    • Journal Title

      J Biol Chem

      Volume: 285 Pages: 30042-9

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PPAR-γ mediates innate immunity by regulating the 1α, 25-dihydroxyvitamin D3 induced hBD-3 and cathelicidin in human keratinocytes2010

    • Author(s)
      Dai X, et al.
    • Journal Title

      J Dermatol Sci

      Volume: 60 Pages: 179-86

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inflammatory mediator TAK1 regulates hair follicle morphogenesis and anagen induction shown by using keratinocyte-specific TAK1-deficient mice.2010

    • Author(s)
      Sayama K
    • Journal Title

      PLoS One

      Volume: 5

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Mite allergen activates inflammasome in epidermal keratinocytes resulting in the production of IL-1beta and IL-182011

    • Author(s)
      Dai X, Sayama K, Tohyama M, Shirakata Y, Hanakawa Y, Tokumaru S, Yang L, Hirakawa S, and Hashimoto K.
    • Organizer
      22^<nd> World Congress of Dermatology
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Mite allergen is a danger signal for the skin2010

    • Author(s)
      Sayama K
    • Organizer
      European Society for Investigative Dermatology
    • Place of Presentation
      ヘルシンキ/フィンランド
    • Year and Date
      2010-09-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mite allergen is a danger signal for the skin2010

    • Author(s)
      Sayama K, Dai X, Shirakata Y, Tohyama M, Miyawaki S, Hirakawa S, and Hashimoto K.
    • Organizer
      40^<th> Annual ESDR Meeting of the European Society for Dermatological Research
    • Place of Presentation
      Helsinki, Finland
    • Related Report
      2011 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi