Analysis of L1 retrotransposition activity in the brains of patients with psychiatric disorders.

• Project/Area Number: 22659214
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Psychiatric science
• Research Institution: The University of Tokyo (2011-2012); The Institute of Physical and Chemical Research (2010)
• Principal Investigator: IWAMOTO Kazuya 東京大学, 医学部附属病院, 特任准教授 (40342753)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,280,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: 死後脳 / 神経細胞 / トランスポゾン / 統合失調症 / 精神疾患 / LINE-1 / 神経発達障害仮説 / 抗精神病薬 / エピジェネティクス / 双極性障害

Research Abstract

We measured LINE-1 copy number in brains of patients with psychiatric disorders by Taqman-based PCR. We identified significant increase of LINE-1 copy number in brains of patients with schizophrenia in two different cohorts. This finding is well concordant with neurodevelopmental model of schizophrenia.

Research Products

• [Journal Article] DNA methylation of the BDNF gene and its relevance to psychiatric disorders (2013)
  • Author(s): Ikegame Tempei, Bundo Miki, Murata Yui, Kasai Kiyoto, Kato Tadafumi, Iwamoto Kazuya
  • Journal Title: Journal of Human Genetics Volume: (印刷中) Issue: 7 Pages: 434-438
  • DOI: 10.1038/jhg.2013.65
  • NAID: 10031190038
  • Peer Reviewed
• [Journal Article] Neuronal cell-type specific DNA methylation patterns of the Cacna1c gene. (2013)
  • Author(s): Nishioka Masaki, Shimada Takafumi, Bundo Miki, Ukai Wataru, Hashimoto Eri, Saito Toshikazu, Kano Yukiko, Sasaki Tsukasa, Kasai Kiyoto, Kato Tadafumi, Iwamoto Kazuya.
  • Journal Title: International Journal of Developmental Neuroscience Volume: 31 Pages: 89-95
  • Peer Reviewed
• [Journal Article] Comprehensive DNA methylation analysis of peripheral blood cells derived from patients with first-episode schizophrenia. (2013)
  • Author(s): Nishioka M, Bundo M, Koike S, Takizawa R, Kakiuchi C, Araki T, Kasai K, Iwamoto K
  • Journal Title: J Hum Genet Volume: 58(2) Issue: 2 Pages: 91-7
  • DOI: 10.1038/jhg.2012.140
  • NAID: 10031156430
  • Peer Reviewed
• [Journal Article] Neuronal cell-type specific DNA methylation patterns of the Cacna1c gene (2013)
  • Author(s): Nishioka Masaki
  • Journal Title: International Journal of Developmental Neuroscience Volume: 31 Issue: 2 Pages: 89-95
  • DOI: 10.1016/j.ijdevneu.2012.11.007
  • Peer Reviewed
• [Journal Article] DNA methylation in schizophrenia: progress and challenges of epigenetic studies. (2012)
  • Author(s): Nishioka Masaki, Bundo Miki, Kasai Kiyoto, Iwamoto Kazuya.
  • Journal Title: Genome Medicine Volume: 4 Issue: 12 Pages: 96-96
  • DOI: 10.1186/gm397
  • Peer Reviewed
• [Journal Article] A systematic evaluation of whole genome amplification of bisulfite-modified DNA. (2012)
  • Author(s): Bundo Miki, Sunaga Fumiko, Ueda Junko, Kasai Kiyoto, Kato Tadafumi, Iwamoto Kazuya.
  • Journal Title: Clinical Epigenetics Volume: 4 Issue: 1 Pages: 22-22
  • DOI: 10.1186/1868-7083-4-22
  • Peer Reviewed
• [Journal Article] Neurons show distinctive DNA methylation profile and higher interindividual variations compared with non-neurons (2011)
  • Author(s): Kazuya Iwamoto
  • Journal Title: Genome Research Volume: (in press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Comprehensive DNA methylation analysis of human peripheral blood leukocytes and lymphoblastoid cell lines. (2011)
  • Author(s): Sugawara Hiroko
  • Journal Title: Epigenetics Volume: (in press)(掲載確定)
  • Peer Reviewed
• [Presentation] 精神疾患患者末梢試料を用いたエピゲノム解析の可能性 (2012)
  • Author(s): 岩本和也
  • Organizer: 第34回日本生物学的精神医学会(招待講演)
  • Place of Presentation: 神戸ポートアイランド、兵庫
  • Year and Date: 2012-09-28
• [Presentation] Neuronal epigenomics in schizophrenia. (2012)
  • Author(s): Iwamoto Kazuya(代表), Bundo Miki, Kasai Kiyoto, Kato Tadafumi.
  • Organizer: 第35回日本神経科学会(招待講演)
  • Place of Presentation: 名古屋国際会議場、愛知
• [Presentation] 双極性障害および統合失調症患者神 経細胞核におけるエピゲノム解析 (2012)
  • Author(s): 岩本和也(代表)、文東美紀、上田順子、 須永史子、根本妙子、笠井清登、加藤忠 史
  • Organizer: 第回エピジェネティクス研究会年会
  • Place of Presentation: 東京一ツ橋学術総合セ ンター、東京
• [Presentation] Epigenomics of brains of patients with bipolar disorder and schizophrenia.3rd Shanghai International Conference of Epigenetics and Diseases (2012)
  • Author(s): Iwamoto Kazuya(代表), Kato Tadafumi.
  • Organizer: 7th Annual Conference of Asian Epigenome Alliance/Genome Medicine Workshop onEpigenetic(-omic)s in Diseases(招待 講演)
  • Place of Presentation: Shanghai,China
• [Presentation] 精神疾患患者末梢試料を用いたエピゲノム解析の可能性 (2012)
  • Author(s): 岩本和也
  • Organizer: 第34回日本生物学的精神医学会
  • Place of Presentation: 神戸国際会議場
  • Invited
• [Presentation] Neuronal epigenomics in schizophrenia (2012)
  • Author(s): Iwamoto Kazuya
  • Organizer: 第35回日本神経科学会
  • Place of Presentation: 名古屋国際会議場
  • Invited
• [Presentation] Epigenome analysis of brains of patients with bipolar disorder. (2012)
  • Author(s): Iwamoto Kazuya
  • Organizer: 2nd Meeting of East Asian Bipolar Forum
  • Place of Presentation: 九州大学
  • Invited
• [Presentation] Epigenomics of brains of patients with bipolar disorder and schizophrenia. (2012)
  • Author(s): Iwamoto Kazuya
  • Organizer: 3rd Shanghai International Conference of Epigenetics and Diseases
  • Place of Presentation: Shanghai、中国
  • Invited
• [Presentation] Epigenetics of bipolar disorder (2011)
  • Author(s): Kazuya Iwamoto
  • Organizer: Epigenetics in Clinical Medicine
  • Place of Presentation: Karolinska Institute, Sweden
  • Year and Date: 2011-04-04
• [Presentation] Bundo Miki, Kasai Kiyoto, Kato Tadafumi. DNA methylation analysis of neuronal nuclei derived from bipolar disorder. (2011)
  • Author(s): Iwamoto Kazuya(代表 )
  • Organizer: 第34回日本神経科学会(招待講演)
  • Place of Presentation: パシフィコ横浜、神奈川
• [Presentation] 精神疾患患者試料を用いた epigenome 解析 (2011)
  • Author(s): 岩本和也
  • Organizer: 大阪大学蛋白質研究所 セミナー(招待講演)
  • Place of Presentation: 大阪大学、大阪
• [Presentation] 脳ゲノムの多様性と精神疾患 (2011)
  • Author(s): 岩本和也(代表)、文東美紀、笠井清登、 加藤忠史
  • Organizer: 第33回日本生物学的精神医学会
  • Place of Presentation: ホテルグランパシフィ ック LE DAIBA、東京
• [Presentation] Epigenetics of bipolar disorder. (2011)
  • Author(s): Iwamoto Kazuya(代表), Kato Tadafumi.
  • Organizer: Epigenetics in clinical Medicine(招 待講演)
  • Place of Presentation: Stockholm,Sweden
• [Presentation] エピジェネティック変異と精神疾患 (2010)
  • Author(s): 岩本和也
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会
  • Place of Presentation: 神戸ポートアイランド・兵庫
  • Year and Date: 2010-12-07
• [Presentation] ヒト死後脳におけるレトロトランスポゾンLINE-1配列のコピー数定量 (2010)
  • Author(s): 文東美紀
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会
  • Place of Presentation: 神戸ポートアイランド・兵庫
  • Year and Date: 2010-12-07
• [Presentation] Promoter-wide DNA methylation analysis in neuronal nuclei derived from brains of patients with psychiatric diseases (2010)
  • Author(s): Kazuya Iwamoto
  • Organizer: 60th American Society of Human Genetics
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2010-11-02
• [Presentation] Aberrant DNA methylation in neuronal nuclei derived from brains of patients with bipolar disorder. (2010)
  • Author(s): Kazuya Iwamoto
  • Organizer: 18th World Congress of Psychiatric Genetics
  • Place of Presentation: Athens, Greece
  • Year and Date: 2010-10-03
• [Presentation] Quantification of the LINE-1 copy number in human postmortem brains (2010)
  • Author(s): Miki Bundo
  • Organizer: 18th World Congress of Psychiatric Genetics
  • Place of Presentation: Athens, Greece
  • Year and Date: 2010-10-03
• [Presentation] 精神疾患患者由来試料を用いた分子病因およびバイオマーカー探索 (2010)
  • Author(s): 岩本和也
  • Organizer: 第18回日本精神行動遺伝医学会
  • Place of Presentation: 東北大学・宮城
  • Year and Date: 2010-09-18
• [Presentation] 精神疾患患者死後脳におけるエピゲノム解析 (2010)
  • Author(s): 岩本和也
  • Organizer: 第20回日本臨床精神薬理学会・第40回日本神経精神薬理学会
  • Place of Presentation: 仙台国際センター・宮城
  • Year and Date: 2010-09-15
• [Presentation] ヒト死後脳ゲノムDNAにおけるレトロトランスポゾンのコピー数定量 (2010)
  • Author(s): 文東美紀
  • Organizer: 第4回エピジェネティクス研究会
  • Place of Presentation: 米子市民文化ホール・鳥取
  • Year and Date: 2010-05-28
• [Presentation] Epigenome analysis of brains of patients with bipolar disorder. (2010)
  • Author(s): Iwamoto Kazuya(代表). Kato Tadafumi.
  • Organizer: 2nd Meeting of East Asian Bipolar Forum(招待講演)
  • Place of Presentation: Kyusyu,Japan
• [Presentation] 精神疾患患者死後脳における エピゲノム解析 (2010)
  • Author(s): 岩本和也
  • Organizer: 第20回日本臨床精神 薬理学会・第40回日本神経精神薬理学会(招待講演)
  • Place of Presentation: 仙台国際センター、宮城
• [Remarks] 分子精神医学講座web site
  • URL: http://www.molpsy.com/
• [Remarks] 所属研究室
  • URL: http://www.molpsy.com/
• [Remarks]
  • URL: http://www.mol.Dsv-com/