The role of cancer stem cells on micrometastasis of colorectal carcinoma
| Project/Area Number |
22659238
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OZAKI Michitaka 北海道大学, 大学院・保健科学研究院, 教授 (80256510)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,190,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | 大腸癌 / 微小転移 / 癌幹細胞 / Pre-metastatic niche / CD133 / premetastatic niche / pre-metastatic niche |
|---|
| Research Abstract |
Recently, two striking concepts,(“premetastatic niche (PMN)” and ”cancer stem cell hypothesis”) that have emerged in the cancer science, have prompted to revise therapeutic strategies for cancer. Our study hypothesized that soluble factors secreted from cancer stem cells promote micrometastasis by the formation of pre-metastatic niche in colorectal carcinoma. The results of our experiments are as follows:1. CD133+ colon cancer cell line has tumorigenicity in the subcutaneous tumor model. 2. PMN formation was observed before micrometastasis in the murine liver metastasis model.3. Conditioned medium of colon cancer cell line generate PMN. 4. Conditioned medium from CD133+ colon cancer cell line enhanced the formation of PMN. 5. Blockade of SDF-1/ CXCR4 axis attenuated PMN formation. These results indicate blockade of SDF-1/ CXCR4 axis attenuated cancer micrometastasis by preventing PMN formation.
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Report
(3 results)
Research Products
(3 results)
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[Presentation] 要望演題2013
Author(s)
大腸癌の骨髄腫瘍細胞はCD133を発現しているか
Organizer
第68回日本消化器外科学会総会
Place of Presentation
シーガイアコンベンションセンター（宮崎県）
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