Generation of intestinal epithelial cell line from induced pluripotent stem cell and establishment of colorectal cancer stem cell model

• Project/Area Number: 22659242
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Hiroshima University
• Principal Investigator: HINOI Takao 広島大学, 病院, 講師 (10444689)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,230,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 小腸大腸肛門外科学 / iPS細胞 / 腸上皮細胞 / 大腸癌幹細胞

Research Abstract

Induced pluripotent stem cell is the ideal stem cell and it is not necessary to use embryonic stem cell. In this study, we aimed to establish normal intestinal epithelium cell line by progenitor of endoderm induced from mouse iPS cells in the first step and differentiated by gene transfer of intestine-specific homeobox transcription factor CDX2. In the next step, we aim to generate iPS-induced colorectal cancer stem cell line with transformation using gene transfer of colorectal cancer-related gene. By analyzing the these cell lines, we aimed to identify novel target of the colorectal cancer-related genes with the microarray analysis.

Research Products

• [Journal Article] Identification of patients likely to benefit from metastasectomy in stage IV colorectal cancer (2012)
  • Author(s): Shimomura M, Okajima M, Hinoi T, Egi H, Takakura Y, Kawaguchi Y, Tokunaga M, Adachi T, Tashiro H, Ohdan H
  • Journal Title: Int J Colorectal Dis Volume: (In press) Issue: 10 Pages: 1339-1346
  • DOI: 10.1007/s00384-012-1454-2
  • Peer Reviewed
• [Journal Article] Adequate lymph node examination isessential to ensure the prognostic valueof the lymph node ratio in patients withstage iii colorectal cancer (2011)
  • Author(s): Shimomura M, Ikeda S, Takakura Y, Kawaguchi Y, Tokunaga M, Egi H, Hinoi T, Okajima M, Ohdan H.
  • Journal Title: Surg Today Volume: 41(10) Pages: 1370-9
  • Peer Reviewed
• [Journal Article] External validation of two nomograms for predicting patient survival after hepatic resection for metastatic colorectal cancer (2011)
  • Author(s): Takakura Y, Okajima M, Kanemitsu Y, Kuroda S, Egi H, Hinoi T, Tashiro H, Ohdan H
  • Journal Title: World J Surg Volume: 35(10) Pages: 2275-82
  • Peer Reviewed
• [Journal Article] Malignant peripheral nerve sheath tumor arising from the greater omentum : case report (2011)
  • Author(s): Miguchi M, Takakura Y, Egi H, Hinoi T, Adachi T, Kawaguchi Y, Shinomura M, Tokunaga M, Okajima M, Ohdan H
  • Journal Title: World J Surg Oncol., Mar Volume: 21 Pages: 9-33
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of colorectal cancer with gastric phenotype : claudin-18 is associated with poor prognosis (2010)
  • Author(s): Matsuda M, Sentani K, Noguchi T, Hinoi T, Okajima M, Matsusaki K, Sakamoto N, Anami K, Naito Y, Oue N, Yasui W
  • Journal Title: Pathol Int Volume: 60(10) Pages: 673-80
  • Peer Reviewed
• [Journal Article] CDX2 regulates multidrug resistance 1 gene expression in malignant intestinal epithelium (2010)
  • Author(s): Takakura Y, Hinoi T, Oue N, SasadaT, Kawaguchi Y, Okajima M, Akyol A, Fearon ER, Yasui W, Ohdan H
  • Journal Title: Cancer Res Volume: 70(17) Pages: 6767-78
  • Peer Reviewed
• [Journal Article] Search for transmembrane protein in gastric cancer by the Escherichia coli ampicillin secretion trap : expression of DSC2 in gastric cancer with intestinal phenotype (2010)
  • Author(s): Anami K, Oue N, Noguchi T, Sakamoto N, Sentani K, Hayashi T, Hinoi T, Okajima M, Graff JM, Yasui W
  • Journal Title: J Pathol Volume: 221(3) Pages: 275-84
  • Peer Reviewed
• [Journal Article] Gastrointestinal stromal tumors of the small intestine in pediatric populations : a case report and literature review (2010)
  • Author(s): Shimomura M, Ikeda S, Takakura Y, Kawaguchi Y, Tokunaga M, Takeda H, Sumitani D, Yoshimitsu M, Hinoi T, Okajima M, Ohdan H
  • Journal Title: Pediatr Surg Int Volume: 26(6) Pages: 649-54
  • Peer Reviewed