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Generation of intestinal epithelial cell line from induced pluripotent stem cell and establishment of colorectal cancer stem cell model

Research Project

Project/Area Number 22659242
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionHiroshima University

Principal Investigator

HINOI Takao  広島大学, 病院, 講師 (10444689)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,230,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywords小腸大腸肛門外科学 / iPS細胞 / 腸上皮細胞 / 大腸癌幹細胞
Research Abstract

Induced pluripotent stem cell is the ideal stem cell and it is not necessary to use embryonic stem cell. In this study, we aimed to establish normal intestinal epithelium cell line by progenitor of endoderm induced from mouse iPS cells in the first step and differentiated by gene transfer of intestine-specific homeobox transcription factor CDX2. In the next step, we aim to generate iPS-induced colorectal cancer stem cell line with transformation using gene transfer of colorectal cancer-related gene. By analyzing the these cell lines, we aimed to identify novel target of the colorectal cancer-related genes with the microarray analysis.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (8 results)

All 2012 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 8 results)

  • [Journal Article] Identification of patients likely to benefit from metastasectomy in stage IV colorectal cancer2012

    • Author(s)
      Shimomura M, Okajima M, Hinoi T, Egi H, Takakura Y, Kawaguchi Y, Tokunaga M, Adachi T, Tashiro H, Ohdan H
    • Journal Title

      Int J Colorectal Dis

      Volume: (In press) Issue: 10 Pages: 1339-1346

    • DOI

      10.1007/s00384-012-1454-2

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adequate lymph node examination isessential to ensure the prognostic valueof the lymph node ratio in patients withstage iii colorectal cancer2011

    • Author(s)
      Shimomura M, Ikeda S, Takakura Y, Kawaguchi Y, Tokunaga M, Egi H, Hinoi T, Okajima M, Ohdan H.
    • Journal Title

      Surg Today

      Volume: 41(10) Pages: 1370-9

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] External validation of two nomograms for predicting patient survival after hepatic resection for metastatic colorectal cancer2011

    • Author(s)
      Takakura Y, Okajima M, Kanemitsu Y, Kuroda S, Egi H, Hinoi T, Tashiro H, Ohdan H
    • Journal Title

      World J Surg

      Volume: 35(10) Pages: 2275-82

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Malignant peripheral nerve sheath tumor arising from the greater omentum : case report2011

    • Author(s)
      Miguchi M, Takakura Y, Egi H, Hinoi T, Adachi T, Kawaguchi Y, Shinomura M, Tokunaga M, Okajima M, Ohdan H
    • Journal Title

      World J Surg Oncol., Mar

      Volume: 21 Pages: 9-33

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical analysis of colorectal cancer with gastric phenotype : claudin-18 is associated with poor prognosis2010

    • Author(s)
      Matsuda M, Sentani K, Noguchi T, Hinoi T, Okajima M, Matsusaki K, Sakamoto N, Anami K, Naito Y, Oue N, Yasui W
    • Journal Title

      Pathol Int

      Volume: 60(10) Pages: 673-80

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] CDX2 regulates multidrug resistance 1 gene expression in malignant intestinal epithelium2010

    • Author(s)
      Takakura Y, Hinoi T, Oue N, SasadaT, Kawaguchi Y, Okajima M, Akyol A, Fearon ER, Yasui W, Ohdan H
    • Journal Title

      Cancer Res

      Volume: 70(17) Pages: 6767-78

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Search for transmembrane protein in gastric cancer by the Escherichia coli ampicillin secretion trap : expression of DSC2 in gastric cancer with intestinal phenotype2010

    • Author(s)
      Anami K, Oue N, Noguchi T, Sakamoto N, Sentani K, Hayashi T, Hinoi T, Okajima M, Graff JM, Yasui W
    • Journal Title

      J Pathol

      Volume: 221(3) Pages: 275-84

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gastrointestinal stromal tumors of the small intestine in pediatric populations : a case report and literature review2010

    • Author(s)
      Shimomura M, Ikeda S, Takakura Y, Kawaguchi Y, Tokunaga M, Takeda H, Sumitani D, Yoshimitsu M, Hinoi T, Okajima M, Ohdan H
    • Journal Title

      Pediatr Surg Int

      Volume: 26(6) Pages: 649-54

    • Related Report
      2011 Final Research Report
    • Peer Reviewed

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Published: 2010-08-23   Modified: 2016-04-21  

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