The study to clarify a mechanism of replacing growth of the hepatocellular carcinoma using the adipose tissue derived stem cells
| Project/Area Number |
22659243
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SHIMADA Mitsuo 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10216070)
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| Co-Investigator(Kenkyū-buntansha) |
UTSUNOMIYA Tohru 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (30304801)
IMURA Satoru 徳島大学, 病院, 助教 (90380021)
MORINE Yuji 徳島大学, 病院, 助教 (60398021)
IKEMOTO Tetsuya 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (20398019)
BANDO Yoshimi 徳島大学, 病院, 准教授 (00238239)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,290,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | 肝細胞癌 / 置換性発育 / 脂肪幹細胞 / 癌幹細胞 |
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| Research Abstract |
We investigated the role of the adipose tissue-derived stem cells(ADSC) in replacing growth of hepatoccellar carcinoma(HCC). HCC cell line(HuH7) and ADSC were co-cultured with or without cell-cell contact to clarify whether mediator affected HCC cell growth. And we concluded cell contact was important process in cell growth during co-culture HCC cells with ADSC. In addition, immunohistochemical staining of stemness marker(CD44 and CD133) and junction protein(Connexin 32) was performed in eleven well differentiated HCC patients with replacing growth. No positive cells were recognized in the invasive front area, however, junction protein(Cx32) was expressed at peripheral area in cytoplasm.
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Report
(3 results)
Research Products
(36 results)
