Clinical importance of aberrant splicing of pre-mRNA encoding a glycolytic enzyme in colorectal cancer and its potential for diagnostic marker
| Project/Area Number |
22659248
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所) (2011)
Miyagi Cancer Center Research Institute (2010) |
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Principal Investigator |
SHIIBA Kenichi 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 特任研究員 (90196345)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Masayuki 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 特任研究員 (00573685)
TANUMA Nobuhiro 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 主任研究員 (40333645)
SATO Ikuro 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), ティッシュ-バンクセンター, センター長 (50225918)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,260,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | 大腸癌 / 遺伝子発現 / スプライシング / 解糖系酵素 / 低酸素 / 遺伝子情報 / ワールブルグ効果 |
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| Research Abstract |
Analysis using clinical speicmens revealed an aberrant pre-mRNA splicing of pyruvate kinase M(PKM) in early phase during colon carcinogenesis. Such splicing dys-regulation was also observed in aberrant crypt foci of animal models. In addition, we found up-regulation of genes encoding several splicing factors, probably eliciting aberrant PKM splicing in colorectal tumor. Knock-down of these splicing factors partially restored PKM splicing-regulation of cell-lines.
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Report
(3 results)
Research Products
(23 results)
