Development of canine iPS cells and new in situ Tissue Engineering for airway reconstruction

• Project/Area Number: 22659250
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: NAKAMURA Tatsuo 京都大学, 再生医科学研究所, 准教授 (70227908)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,190,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: iPS細胞 / 人工気管 / コラーゲン / 人工臓器 / 生体内再生 / ステント / 再生医学 / 気管 / 気道 / 再生 / 組織工学 / 胸部外科 / 呼吸器 / 肺癌 / iPS / 組織幹細胞 / 機能再建

Research Abstract

We challenged to make an iPS cell from a somatic cell of beagle dog using 4 Yamanaka factors(Oct3, Sox2, Klf4, c-Myc) that were induced by retrovirus, however constant silencing has not yet been recognized.
With regard to the regeneration scaffold for airway, new tracheal prosthesis was manufactured with bioabsorbable vicryl mesh reinforced with polypropylene stent with a diameter of 1 mm. In animal experiment in canine, it was determined that conquer of dehiscence between mesh and stent is of importance after the implantation.

Research Products

• [Journal Article] Transforming Growth Factor/33 for the prevention of vocal fold scarring (2012)
  • Author(s): Ohno, S., Hirano, S., Kanemaru, S., Kitani, Y., Kojima, T., Ishikawa, S., Mizuta, M., Tateya, I., Nakamura, T., Ito, J.
  • Journal Title: Laryngoscope Volume: 122 Issue: 3 Pages: 583-589
  • DOI: 10.1002/lary.22389
  • Peer Reviewed
• [Journal Article] Accelerated generation of human induced pluripotent stem cells with retroviral transduction and chemical inhibitors under physiological hypoxia (2012)
  • Author(s): Shimada, H., Hashimoto, Y., Nakada, A., Shigeno, K., Nakamura, T.
  • Journal Title: BBRC Volume: 417 Issue: 2 Pages: 659-664
  • DOI: 10.1016/j.bbrc.2011.11.111
  • Peer Reviewed
• [Journal Article] Reduction of air leaks in a canine model of pulmonary resection with a new staple-line buttress (2011)
  • Author(s): Hashimoto, A., Kuwabara, M., Hirasaki, Y., Tsujimoto, H., Torii, T., Nakamura, T., Hagiwara, A.
  • Journal Title: J Thorac Cardiovasc Surg Volume: 142 Issue: 2 Pages: 366-371
  • DOI: 10.1016/j.jtcvs.2011.05.001
  • Peer Reviewed
• [Journal Article] The protective efficacy of basic fibroblast growth factor in radiation-induced salivary gland dysfunction in mice (2011)
  • Author(s): Kojima, T., Kanemaru, S., Hirano, S., Tateya, I., Suehiro, A., Kitani, Y., Kishimoto, Y., Ohno, S., Nakamura, T., Ito, J.
  • Journal Title: Laryngoscope Volume: 121 Issue: 9 Pages: 1870-1875
  • DOI: 10.1002/lary.21873
  • Peer Reviewed
• [Journal Article] Regeneration of radiation damaged salivary glands with adipose-derived stromal cells (2011)
  • Author(s): Kojima, T., Kanemaru, S., Hirano, S., Tateya, I., Ohno, S., Nakamura, T., Ito, J.
  • Journal Title: Laryngoscope Volume: 121 Issue: 9 Pages: 1864-1869
  • DOI: 10.1002/lary.22080
  • Peer Reviewed
• [Journal Article] Total cricoidectomy and laryngotracheal reconstruction for subglottic stenosis with glottic involvement (2011)
  • Author(s): Yamamoto, K.
  • Journal Title: Interact Cardiovasc Thorac Surg Volume: 13 Issue: 3 Pages: 351-353
  • DOI: 10.1510/icvts.2011.274357
  • Peer Reviewed
• [Journal Article] Meta-analysis of therapeutic procedures for acquired subglottic stenos is in adults (2011)
  • Author(s): Yamamoto, K., Kojima, F., Tomiyama, K., Nakamura, T., Hayashino, Y.
  • Journal Title: Ann Thorac Surg Volume: 91 Issue: 6 Pages: 1747-1753
  • DOI: 10.1016/j.athoracsur.2011.02.071
  • Peer Reviewed
• [Journal Article] Prevention of buccal mucosa scarring with transforming growth factor 3 3 (2011)
  • Author(s): Ohno, S., Hirano, S., Kanemaru, S., Tateya, I., Kitani, Y., Kojima, T., Nakamura, T., Ito, J.
  • Journal Title: Laryngoscope Volume: 121 Issue: 7 Pages: 1404-1409
  • DOI: 10.1002/lary.21759
  • Peer Reviewed
• [Journal Article] Tissue-engineered airway and "in situ tissue engineering" (2011)
  • Author(s): Nakamura, T., Omori, K., Kanemaru, S.
  • Journal Title: Gen Thorac Cardiovasc Surg Volume: 59 Issue: 2 Pages: 91-97
  • DOI: 10.1007/s11748-010-0677-9
  • NAID: 10029018390
  • Peer Reviewed
• [Journal Article] 肺における再生医療 (2011)
  • Author(s): 中村達雄
  • Journal Title: Clinical Engineering Volume: 22 Pages: 32-36
• [Journal Article] Use of adipose-derived stromal cells for prevention of esophageal stricture after circumferential EMR in a canine model (2011)
  • Author(s): Honda, M.
  • Journal Title: Gastrointestinal Endoscopy Volume: 73 Issue: 4 Pages: 777-784
  • DOI: 10.1016/j.gie.2010.11.008
  • Peer Reviewed
• [Journal Article] Development of a novel ant adhesive material, alginate flakes, ex vivo and in vivo (2011)
  • Author(s): Hirasaki, Y., Fukunaga, M., Kidokoro, A., Hashimoto, A., Nakamura, T., Tsujimoto, H., Hagiwara, A.
  • Journal Title: Surg today Volume: 41 Issue: 7 Pages: 970-977
  • DOI: 10.1007/s00595-010-4410-1
  • Peer Reviewed
• [Journal Article] The tissue-engineered airway and "in situ tissue engineering" (2011)
  • Author(s): Nakamura, T.
  • Journal Title: Gen Thorac Cardiovasc Surg Volume: 59 Pages: 91-97
  • NAID: 10029018390
  • Peer Reviewed
• [Journal Article] Use of adipose-derived stromal cells for prevention of esophageal stricture after circumferential EMR in a canine model. (2011)
  • Author(s): Honda, M.
  • Journal Title: Gastrointestinal Endoscopy. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Development of a novel anti-adhesive material, alginate flakes, exvivo and in vivo. (2011)
  • Author(s): Hirasaki, Y.
  • Journal Title: Surg today Volume: (in press)
  • Peer Reviewed
• [Journal Article] Glottal reconstruction with a tissue engineering technique using polypropylene mesh : a canine experiment (2010)
  • Author(s): Yamashita, M., Kanemaru, S., Hirano, S., Umeda, H., Kitani, Y., Omori, K., Nakamura, T., Ito, J
  • Journal Title: Ann Otol Rhinol Laryngol Volume: 119(2) Pages: 110-117
  • URL: http://search.proquest.com/docview/217943107/fulltextPDF?accountid=11929
  • Peer Reviewed
• [Journal Article] Process of healing of mucosal defects in the esophagus after endoscopic mucosal resection : histological evaluation in a dog model (2010)
  • Author(s): Honda, M., Nakamura, T., Hori, Y., Shionoya, Y., Nakada, A., Sato, T., Yamamoto, K., Kobayashi, T., Shimada, H., Kida, N., Hashimoto, A., Hashimoto, Y
  • Journal Title: Endoscopy Volume: 42 Pages: 1092-1095
  • Peer Reviewed
• [Journal Article] Observation of the esophagus, pharynx and lingual root by gastrointestinal endoscopy with a percutaneous retrograde approach (2010)
  • Author(s): Honda, M., Hori, Y., Shionoya, Y., Nakada, A., Sato, T., Kobayashi, T., Shimada, H., Kida, N., Nakamura, T.
  • Journal Title: World J Gastrointest Endosc Volume: 16 Issue: 8 Pages: 288-292
  • DOI: 10.4253/wjge.v2.i8.288
  • Peer Reviewed
• [Journal Article] Male sexual dysfunction after rectal cancer surgery (2010)
  • Author(s): Nishizawa, Y., Saito, N., Ito, M., Sugito, M., Kobayashi, A., Nishizawa, Y
  • Journal Title: Colorectal Dis Volume: 12 Pages: 8-8
  • Peer Reviewed
• [Journal Article] Treatment of acute vocal fold scar with local injection of basic fibroblast growth factor : a canine study (2010)
  • Author(s): Suehiro, A., Hirano, S., Kishimoto, Y., Rousseau, B., Nakamura, T., Ito, J.
  • Journal Title: Acta Otolaryngol Volume: 130 Issue: 7 Pages: 844-850
  • DOI: 10.3109/00016480903426618
  • Peer Reviewed
• [Journal Article] Biodegradable polymer coating promotes the epithelization of tissue-engineered airway prostheses (2010)
  • Author(s): Sato, T., Araki, M., Nakajima, N., Omori, K., Nakamura, T.
  • Journal Title: J Thorac Carciovasc Surg Volume: 139 Issue: 1 Pages: 26-31
  • DOI: 10.1016/j.jtcvs.2009.04.006
  • Peer Reviewed
• [Journal Article] A tissue-engineered trachea derived from a framed collagen scaffold gingival fibroblasts and adipose-derived stem cells (2010)
  • Author(s): Kobayashi, K., Suzuki, T., Nomoto, Y., Tada, Y., Miyake, M., Hazama, A., Wada, I., Nakamura, T., Omori, K
  • Journal Title: Biomaterials Volume: 31 Issue: 18 Pages: 4855-4863
  • DOI: 10.1016/j.biomaterials.2010.02.027
  • Peer Reviewed
• [Journal Article] Intratracheal catheter suction removes the same volume of me conium with less impact on desaturatio compared with me conium aspirator in me conium aspiration syndrome (2010)
  • Author(s): Akazawa, Y., Ishida, T., Baba, A., Hiroma, T., Nakamura, T.
  • Journal Title: Early Hum Dev. Volume: 86 Issue: 8 Pages: 499-502
  • DOI: 10.1016/j.earlhumdev.2010.06.011
  • Peer Reviewed
• [Journal Article] Potential of induced pluripotent stem cells for the regeneration of the tracheal wall (2010)
  • Author(s): Imaizumi, M., Nomoto, Y., Sugino, T., Miyake, M., Wada, I., Nakamura, T., Omori, K
  • Journal Title: Ann Otol Rhinol Laryngol Volume: 119 Pages: 697-703
  • URL: http://search.proquest.com/docview/759349417/fulltextPDF?accountid=11929
  • Peer Reviewed
• [Journal Article] Atissue-engineering approach for stenosis of the trachea and/orcricoid (2010)
  • Author(s): Kanemaru, S., Hirano, S., Umeda, H., Yamashita, M., Suehiro, A., Nakamura, T., Maetani, T., Omori, K., Ito, J
  • Journal Title: Acta Otolaryngol Suppl Volume: 130 Pages: 79-83
  • Peer Reviewed
• [Journal Article] 人工神経の基礎と臨床 (2010)
  • Author(s): 中村達雄
  • Journal Title: Peripheral Nerve Volume: 21 Pages: 192-196
  • NAID: 10027742202
• [Journal Article] in situ Tissue Engineeringを用いた気管・気管支の再建 (2010)
  • Author(s): 中村達雄、大森孝一、金丸眞一
  • Journal Title: 日本気管食道科学会会報 Pages: 61-89
  • NAID: 10026600858
• [Journal Article] in situ Tissue Engineeringを用いた気管・気管支の再建 (2010)
  • Author(s): 中村達雄
  • Journal Title: 日本気管食道科学会会報 Volume: 61 Pages: 89-89
  • NAID: 10026600858
• [Journal Article] A tissue-engineering approach for stenosis of the trachea and/or cricoid. (2010)
  • Author(s): Kanemaru, S.
  • Journal Title: Acta Otolaryngol Suppl. Volume: 130 Pages: 79-83
  • Peer Reviewed
• [Journal Article] Potential of induced pluripotent stem cells for the regeneration of the tracheal wall. (2010)
  • Author(s): Imaizumi, M.
  • Journal Title: Ann Otol Rhinol Laryngol. Volume: 119 Pages: 697-703
  • Peer Reviewed
• [Journal Article] Intratracheal catheter suction removes the same volume of meconium with less impact on desaturatio compared with meconium aspirator in meconium aspiration syndrome. (2010)
  • Author(s): Akazawa, Y.
  • Journal Title: Early Hum Dev. Volume: 86 Pages: 499-502
  • Peer Reviewed
• [Journal Article] A tissue-engineered trachea derived from a framed collagen scaffold gingival fibroblasts and adipose-derived stem cells. (2010)
  • Author(s): Kobayashi, K.
  • Journal Title: Biomaterials Volume: 31 Pages: 485-63
  • Peer Reviewed
• [Journal Article] Biodegradable polymer coating promotes the epithelization of tissue-engineered airway prostheses. (2010)
  • Author(s): Sato, T.
  • Journal Title: J Thorac Carciovasc Surg Volume: 139 Pages: 26-31
  • Peer Reviewed
• [Journal Article] Treatment of acute vocal fold scar with local injection of basicfibroblast growth factor : a canine study. (2010)
  • Author(s): Suehiro, A.
  • Journal Title: Acta Otolaryngol. Volume: 130 Pages: 844-50
  • Peer Reviewed
• [Journal Article] Male sexual dysfunction after rectal cancer surgery. (2010)
  • Author(s): Nishizawa, Y.
  • Journal Title: Colorectal Dis. Volume: 12 Pages: 8-8
  • Peer Reviewed
• [Journal Article] Observation of the esophagus, pharynx and lingual root by gastrointestinal endoscopy with a percutaneous retrograde approach. (2010)
  • Author(s): Honda, M.
  • Journal Title: World J Gastrointest Endosc. Volume: 16 Pages: 288-292
  • Peer Reviewed
• [Journal Article] Process of healing of mucosal defects in the esophagus after endoscopic mucosal resection : histological evaluation in a dog model. (2010)
  • Author(s): Honda, M.
  • Journal Title: Endoscopy. Volume: 42 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Glottal reconstruction with a tissue engineering technique using polypropylene mesh : a canine experiment. (2010)
  • Author(s): Yamashita, M.
  • Journal Title: Ann Otol Rhinol Laryngol. Volume: 119 Pages: 110-7
  • Peer Reviewed
• [Presentation] Investigation of optimized conditions for RNA-based cellular reprogramming (2011)
  • Author(s): Shimada, H., Hashimoto, Y., Nakada, A., Shigeno, K., Nakamura, T
  • Organizer: ISSCR
  • Place of Presentation: Toronto(Canada)
• [Presentation] Investigation of optimized conditions for RNA-based cellular reprogramming (2011)
  • Author(s): Shimada, H.
  • Organizer: ISSCR
  • Place of Presentation: Toronto (Canada)
• [Presentation] Autologous transplantation of adipose-derived stromal cells prevent esophageal stricture after endoscopic mucosal resection in a canine model (2010)
  • Author(s): Honda, M., Hori, Y., Nakada, A., Uji, M., Nishizawa, Y., Yamamoto, K., Kobayashi, T., Shimada, H., Kida, N., Sato, T., Nakamura, T
  • Organizer: 第63回日本胸部外科学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-26
• [Presentation] Autologous transplantation of adipose-derived stromal cells prevent esophageal stricture after endoscopic mucosal resection in acanine model. (2010)
  • Author(s): Honda, M.
  • Organizer: 第63回日本胸部外科学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-26
• [Presentation] 食道ESD後の創傷治癒過程と狭窄予防に対する再生医療の応用 (2010)
  • Author(s): 本多通孝、中田顕、中村達雄
  • Organizer: シンポジウム「EMR、ESDの課題安全確実な内視鏡医療の提供に向けて」第18回日本消化器関連学会週間
  • Place of Presentation: 横浜
  • Year and Date: 2010-10-15
• [Presentation] 食道ESD後の創傷治癒過程と狭窄予防に対する再生医療の応用 (2010)
  • Author(s): 本多通孝
  • Organizer: シンポジウム「EMR、ESDの課題 安全確実な内視鏡医療の提供に向けて」第18回日本消化器関連学会週間
  • Place of Presentation: 横浜
  • Year and Date: 2010-10-15
• [Presentation] 食道内視鏡治療後の狭窄予防にステロイド、マイトマイシンCは有効か (2010)
  • Author(s): 本多通孝、中村達雄、山本一道、堀義生、中田顕、塩谷伊毅、小林丈士、島田英徳、木田直樹
  • Organizer: 第18回日本消化器関連学会週間
  • Place of Presentation: 横浜
  • Year and Date: 2010-10-14
• [Presentation] アルコールブロック後のイヌ星状神経節の組織学的検討とホルネル徴候の変化 (2010)
  • Author(s): 塩谷伊毅、砂田勝久、中村達雄
  • Organizer: 第38回日本歯科麻酔学会総会・学術集会(ポスター)
  • Place of Presentation: 横須賀
  • Year and Date: 2010-10-09
• [Presentation] アルコールブロック後のイヌ星状神経節の組織学的検討とホルネル徴候の変化 (2010)
  • Author(s): 塩谷伊毅
  • Organizer: 第38回 日本歯科麻酔学会総会・学術集会
  • Place of Presentation: 横須賀
  • Year and Date: 2010-10-09
• [Presentation] 運動器疼痛疾患への外科的治療の可能性について-適応と限界- (2010)
  • Author(s): 稲田有史
  • Organizer: 第2回備後運動器疼痛疾患研究会
  • Place of Presentation: 福山
  • Year and Date: 2010-09-25
• [Presentation] Transplantation of adipose-derived stromal cells prevent esophageal stricture after endoscopic mucosal resection in canine model (2010)
  • Author(s): Honda, M., Hori, Y., Nakada, A., Uji, M., Nishizawa, Y., Yamamoto, K., Kobayashi, T., Shimada, H., Kida, N., Sato, T., Nakamura, T.
  • Organizer: European Society for Artificial Organs 37^(th) Congress
  • Place of Presentation: Skopje(Macedonia)
  • Year and Date: 2010-09-10
• [Presentation] Transplantation of adipose-derived stromal cells prevent esophageal stricture after endoscopic mucosal resection in canine model. (2010)
  • Author(s): Honda, M.
  • Organizer: European Society for Artificial Organs 37^<th> Congress
  • Place of Presentation: Macedonia
  • Year and Date: 2010-09-10
• [Presentation] 人工神経の基礎と臨床 (2010)
  • Author(s): 中村達雄、萩原明於、稲田有史、金丸眞一
  • Organizer: 第21回末梢神経学会学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-04
• [Presentation] 脂肪由来幹細胞を用いた食道ESD後狭窄の予防 (2010)
  • Author(s): 本多通孝、堀義生、中村達雄、山本一道、中田顕、塩谷伊毅、小林丈士、島田英徳、木田直樹
  • Organizer: 第64回日本食道学会
  • Place of Presentation: 久留米
  • Year and Date: 2010-09-01
• [Presentation] 末梢神経に対する生体内再生治療 (2010)
  • Author(s): 稲田有史
  • Organizer: 第40回新潟神経学夏期セミナー
  • Place of Presentation: 新潟
• [Presentation] 四肢外傷における末梢神経の再建 (2010)
  • Author(s): 稲田有史
  • Organizer: 第2回日本重度四肢外傷セミナー
  • Place of Presentation: 札幌
• [Presentation] Expression of side population cells during wound healing of rat vocal folds (2010)
  • Author(s): Kojima T
  • Organizer: ICALB and ICVPB
  • Place of Presentation: Madison(USA)
• [Presentation] Reprogramming of canine adult somatic cells to induced pluripotent stem cells with retroviral transduction and chemical inhibitors under hypoxic conditions (2010)
  • Author(s): Shimada, H., Nakada, A., Hashimoto, Y., Shigeno, K., Nakamura, T.
  • Organizer: ISSCR(国際幹細胞学会)
  • Place of Presentation: SanFrancisco(USA)
• [Presentation] The Protective Efficacy of Basic Fibroblast Growth Factor in Radiation Damaged Salivary Glands of Mouse (2010)
  • Author(s): Kojima T
  • Organizer: The113^(th) Annual Meeting of Triological Society
  • Place of Presentation: LasVegas(USA)
• [Presentation] 超低位直腸癌に対するISRの適応に関する再検討 (2010)
  • Author(s): 齋藤典男、杉藤正典、伊藤雅昭、小林昭広、西澤雄介、西澤祐吏、皆川のぞみ、中嶋健太郎、渡辺和宏、甲田貴丸、錦織英知、神山篤史
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋
• [Presentation] 内肛門括約筋切除術の病理組織学的剥離面陽性例と再発の検討 (2010)
  • Author(s): 皆川のぞみ、小嶋基寛、伊藤雅昭、杉藤正典、小林昭広、西澤雄介、西澤祐吏、中嶋健太郎、渡辺和宏、甲田貴丸、錦織英知、神山篤史
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋
• [Presentation] Reprogramming of canine adult somatic cells to induced pluripotent stem cells with retroviral transduction and chemical inhibitors under hypoxic conditions. (2010)
  • Author(s): Shimada, H
  • Organizer: ISSCR(国際幹細胞学会)
  • Place of Presentation: San Francisco
• [Presentation] The Protective Efficacy of Basic Fibroblast Growth Factor in Radiation Damaged Salivary Glands of Mouse. (2010)
  • Author(s): Kojima T.
  • Organizer: The 113th Annual Meeting of Triological Society
  • Place of Presentation: USA
• [Presentation] Expression of side population cells during wound healing of ratvocal folds. (2010)
  • Author(s): Kojima T.
  • Organizer: ICALB and ICVPB
  • Place of Presentation: USA
• [Remarks]
  • URL: http://www.frontier.kyoto-u.ac.jp/ca04/index.html
• [Remarks]
  • URL: http://www.frontier.kyoto-u.ac.jp/ca04/index.html
• [Remarks]
  • URL: http://www.frontier.kyoto-u.ac.jp/ca04/index.html