| Project/Area Number |
22659253
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
OKADA Morihito 広島大学, 原爆放射線医科学研究所, 教授 (70446045)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,190,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | 腫瘍学 / 肺がん / がん悪性度診断 / EGFR / 肺腺癌 |
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| Research Abstract |
To develop an ultra-sensitive detection method for EGFR gene mutations in lung cancer, search for the patterns of low-molecular-weight cellular component by video-mass-scope was performed. Using the extract in the cells obtained from cultured cell populations, comprehensive analysis with liquid chromatography mass spectrometry was carried out for high sensitivity of low molecular patterns. In cell lines with EGFR mutations, carnitine, which is involved in the transport of fatty acids, glucose, MFN1 and actin-related protein 2/ 3 complex subunit 3 are present specifically. The proteins and amino acids can be related to EGFR mutations. Video-mass-scope is suggested to be applied as an ultra-sensitive detection method for EGFR mutations in lung cancer.
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