| Project/Area Number |
22659263
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
YANO Shigetoshi 熊本大学, 大学院・生命科学研究部, 准教授 (60332871)
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| Co-Investigator(Kenkyū-buntansha) |
HIDE Takuichiro 熊本大学, 医学部附属病院, 助教 (40421820)
KURATSU Jun-ichi 熊本大学, 大学院・生命科学研究部, 教授 (20145296)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥2,230,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | 下垂体腺腫 / 腫瘍幹細胞 / 内視鏡手術 / 下垂体ホルモン / 機能性腺腫 |
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| Research Abstract |
To investigate the presence of tumor stem cells in the pituitary adenoma, and to clarifythe relationship of tumor diversity and tumor development, we cultured pituitary tumorstem cells from surgical resected specimen. Stable sphere formation was obtained in twocases of non-functioning pituitary adenoma, one case of GH-secreting pituitary adenoma,and one case of ACTH-secreting pituitary adenoma. In the condition of differentiationmedia, sphere cells obtained from GH-secreting pituitary adenoma or ACTH-secretingpituitary adenoma were positively immunostained with anti-GH antibody or anti-ACTHantibody, respectively. When inducing differentiation of pituitary adenoma stem cellsextracted from the non functional pituitary adenoma was performed, positive PRL and GHimmunostaining became to be observed. Furthermore, the presence of Nestin-positive cellswas observed in tumor tissue. From these results, the pituitary tumor stem cells may existin the tumor tissue and have the ability to differentiate into multiple directions
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