| Project/Area Number |
22659272
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KATO Tomohisa 京都大学, 再生医科学研究所, 講師 (50301247)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,130,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | 骨軟部肉腫 / 人工多能性幹細胞 / 遺伝子発現誘導 / 融合遺伝子 |
|---|
| Research Abstract |
The object of this project is to identify the cell-of-origin of sarcomas using human iPS cells. Using drug-inducible expression vector and transposes-supported gene introduction system, we successfully established genetically modified iPS cells. Without drug-treatment these cells retained undifferentiated state. When the SYT-SSX gene was induced, however, the expression level of stem cell markers were down-regulated and at the same time those of lineage-specific genes were up-regulated, suggesting the involvement of SYT-SX protein in the process of differentiation.
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