| Project/Area Number |
22659293
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Akita University |
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Principal Investigator |
TERADA Yukihiro 秋田大学, 大学院・医学系研究科, 教授 (10260431)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | 卵子発生能 / 胚性遺伝子 / 遺伝子導入 / 体外受精 / 卵子胚生能 |
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| Research Abstract |
The embryonic genes such as Oct3/4 function for genomic initialization. Recently, it is suggested that embryonic genes function for the oocyte/embryonal development. We examined whether improvement of the ability for embryonic development by introduction of these embryonic genes. We made cRNA+GFP probe of the each Oct3/4, Sox2, Nanog and examined functions in in vivo and in vitro. Furthermore, we evaluated embryonal developmental ability of the egg after introduction of these cRNAs. The tendency that embryonic developmental ability improved after introduction of cRNAs, was found. However, we did not obtain clear conclusions about the developmental improvement of oocytes from an aging individual.
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