| Project/Area Number |
22659298
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HORIUCHI Akiko 信州大学, 医学部附属病院, 特任研究員 (80334895)
SUZUKI Akihisa 信州大学, 医学部, 助教 (10547095)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,530,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | 遺伝子損傷 / TDPCR / 子宮内膜癌 / サイクリン / 子官内膜癌 |
|---|
| Research Abstract |
The involvement of estrogen in endometrial carcinogenesis is controversial、therefore, we intended to examine estrogen or its metabolite could induce DNA damage on endometrial cells by using terminal transferase dependent PCR(TDPCR) method. At first, we modified TDPCR by applying a capillary sequencer and consequently we could avoid its complicated, time-consuming process. By using modified TDPCR, we found that catechol estrogen caused DNA damages on frequently mutated gene in endometrial carcinoma, PTEN, while estrogen did not. In addition, the foci of DNA damage corresponded to known mutation site of PTEN in endometrial carcinoma. Taken together, these data suggest that metabolite of estrogen is responsible for PTEN mutations in endometrial carcinogenesis.
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