| Project/Area Number |
22659304
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | National Research Institute for Child Health and Development |
|---|
Principal Investigator |
AKUTSU Hidenori 独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 室長 (50347225)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TOYODA Masashi 地方独立行政法人東京都健康長寿医療センター, 研究所老年病研究チーム・血管医学研究, 研究副部長 (50392486)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,460,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥600,000 (Direct Cost: ¥600,000)
|
|---|
| Keywords | リプログラミング / 幹細胞 / 受精卵 / 発生 / 受精 / マイクロアレイ / 初期化 / マイクロインジェクション |
|---|
| Research Abstract |
Nuclear reprogramming means that either functional or molecular changes to cells undergoing fate changes, and the mechanism elucidation would deliver great advance not only for the basics of biological systems also more applicable field such as medicine and drug development. In this study, mouse zygotes are focused to be elucidated reprogramming. In early stage of the mammalian preimplantation development, most dramatic epigenetic change is observed the pronuclear formation through their fusion process. Female and male pronuclear materials were collected by micromanipulation technique and were done by the global transcriptional expression analysis using linear amplified RNAs. Gene cluster analysis in conjunction with reprogramming in silico was performed. It was able to extract a convincing gene mainly on a gene in conjunction with the chromatin reprogramming.
|
