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New strategy for seasonal allergic rhinitis by exosomes

Research Project

Project/Area Number 22659306
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionUniversity of Fukui

Principal Investigator

FUJIEDA Shigeharu  福井大学, 医学部, 教授 (30238539)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,140,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsエクソゾーム / スギ花粉症 / MHC class II / CD9 / CD86 / 細胞間連絡物質 / MHC classII alpha鎖 / CryJ1 / ペプチド / 遺伝情報 / 蔗糖密度勾配 / 樹状細胞
Research Abstract

Exosomes are complex structures composed of a lipid bilayer that contains transmembrane proteins and encloses soluble hydrophilic components derived from the cytosol of the donor cells. They also contain the information of antigens with MHC class I & II. These molecules would be taken by neighboring cells and stimulate the receptors in the surface of cells. In this study we examined whether isolated exosomes from good responder of sublingual immunotherapy for allergic rhinitis(Japanese cedar pollen) could have an effect on immune systems. We got 15 to 80μg exosomes from the human sera. Western blots demonstrated that exosomes had MHC class IIαchain, CD9 and CD86 molecules. No band was found by anti-Cry J1(Japanese cedar pollen antigen) antibody in western blots. This study showed that we could isolate exosomes from human sera. However, we need more improvement methods to get high volume of exosomes and sensitivity to detect them for the advanced study.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (18 results)

All 2012 2011

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (5 results)

  • [Journal Article] Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps2012

    • Author(s)
      Takabayashi T, Kato A, et al.
    • Journal Title

      J Allergy Clin Immunol

    • URL

      http://dx.doi.org/10.1016/j.jaci.2012.02.046

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A critical role of IL-33 in experimental allergic rhinitis2012

    • Author(s)
      Haenuki Y, Matsushita K, et al.
    • Journal Title

      J Allergy Clin Immunol

    • URL

      http://dx.doi.org/10.1016/j.jaci.2012.02.013

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Examination, diagnosis and classification for Japanese allergic rhinitis : Japanese guideline2012

    • Author(s)
      Fujieda S, Kurono Y, et al.
    • Journal Title

      Auris Nasus Larynx

    • URL

      http://dx.doi.org/10.1016/j.anl.2011.12.006

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of inhaled antigen sensitization and nasal eosinophils in Japanese children under two years old2012

    • Author(s)
      Osawa Y, Suzuki D, et al.
    • Journal Title

      Int J Pediatr Otorhinolaryngol

      Volume: 76(2) Pages: 189-93

    • URL

      http://dx.doi.org/10.1016/j.ijporl.2011.10.024

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficacy of mometasone furoate nasal spray for nasal symptoms, quality of life, rhinitis-disturbed sleep, and nasal nitric oxide in patients with perennial allergic rhinitis2012

    • Author(s)
      Yamada T, Yamamoto H, et al.
    • Journal Title

      Allergy Asthma Proc

      Volume: 33(2) Pages: 9-16

    • URL

      http://dx.doi.org/10.2500/aap.2012.33.3509

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficacy of prophylactic treatment with montelukast and montelukast plus add-on loratadine for seasonal allergic rhinitis2012

    • Author(s)
      Yamamoto H, Yamada T, et al.
    • Journal Title

      Allergy Asthma Proc

      Volume: 33(2) Pages: 17-22

    • URL

      http://dx.doi.org/10.2500/aap.2012.33.3514

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] アレルギー性鼻炎2012

    • Author(s)
      藤枝重治、伊藤有未
    • Journal Title

      アレルギー

      Volume: 61(2) Pages: 168-173

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ORAI1 genetic polymorphisms associated with the susceptibility of atopic dermatitis in Japanese and Taiwanese populations.2012

    • Author(s)
      Chang WC, Lee CH, Hirota T, Wang LF, Doi S, Miyatake A, Tomita K, Sakashita M, Yamada T, et al.
    • Journal Title

      PLoS One.

      Volume: 7 Issue: 1 Pages: e29387-e29387

    • DOI

      10.1371/journal.pone.0029387

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of inhaled antigen sensitization and nasal eosinophils in Japanese children under two years old2012

    • Author(s)
      Osawa Y, Suzuki D, Ito Y, Narita N, Ohshima Y, Ishihara Y, Ishihara Y, Tsuchida S, Fujieda S
    • Journal Title

      Int J Pediatr Otorhinolaryngol

      Volume: Vol.76 Issue: 2 Pages: 189-193

    • DOI

      10.1016/j.ijporl.2011.10.024

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interleukin-19 Downregulates Interleukin-4-Induced Eotaxin Production in Human Nasal Fibroblasts2011

    • Author(s)
      Higashino M, Takabayashi T, et al.
    • Journal Title

      Allergology International

      Volume: 60 Issue: 4 Pages: 449-457

    • DOI

      10.2332/allergolint.10-OA-0262

    • NAID

      130004477105

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Platelet Derived Endothelial Cell Growth Factor/Thymidine Phosphorylase Enhanced Human IgE Production2011

    • Author(s)
      Yamamoto H, Yamada T, et al
    • Journal Title

      Allergology International

      Volume: 60 Issue: 1 Pages: 79-85

    • DOI

      10.2332/allergolint.10-OA-0220

    • NAID

      10031143302

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Upregulation of IL17RB during Natural Allergen Exposure in Patients with Seasonal Allergic Rhinitis2011

    • Author(s)
      Matsumoto Y, Noguchi E, et al.
    • Journal Title

      Allergology International

      Volume: 60 Issue: 1 Pages: 87-92

    • DOI

      10.2332/allergolint.10-OA-0230

    • NAID

      10031143303

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Btype CpG-DNA suppresses poly(I : C)-induced BLyS expression and production in human tonsillar fibroblasts2011

    • Author(s)
      Yamada T, Jiang X, et al.
    • Journal Title

      Clin Immunol

      Volume: 141(3) Pages: 365-71

    • URL

      http://dx.doi.org/10.1016/j.clim.2011.09.012

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] New therapeutic strategy for allergic rhinitis2011

    • Author(s)
      S.Fujieda
    • Organizer
      11th Japan-Taiwan Conference on Otolaryngology-Head and Neck Surgery
    • Place of Presentation
      Kobe, Japan(招待講演)
    • Year and Date
      2011-12-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] New clinical marker for allergic rhinitis2011

    • Author(s)
      Fujieda S
    • Organizer
      14th International Rhinology Society & 30th International Symposium on Infection and Allergy of the Nose
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] New clinical marker for allergic rhinitis2011

    • Author(s)
      S.Fujieda
    • Organizer
      14th International Rhinology Society & 30th International Symposium on Infection and Allergy of the Nose
    • Place of Presentation
      Tokyo, Japan(招待講演)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Association between genetic variant of interleukin-33 and seasonal allegic rhinitis in the Japanese population2011

    • Author(s)
      Fujieda S, et al.
    • Organizer
      Collegium Oro-Rhio-Laryngologyg-icum Amicitiae Sacrum
    • Place of Presentation
      Bruges, Belgium
    • Year and Date
      2011-09-06
    • Related Report
      2011 Final Research Report
  • [Presentation] Association between genetic variant of interleukin-33 and seasonal allegic rhinitis in the Japanese population2011

    • Author(s)
      S.Fujieda
    • Organizer
      Collegium Oro-Rhio-Laryngologygicum Amicitiae Sacrum
    • Place of Presentation
      Bruges, Belgium
    • Year and Date
      2011-09-06
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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