| Project/Area Number |
22659308
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Otorhinolaryngology
|
|---|
| Research Institution | Ehime University |
|---|
Principal Investigator |
GYO Kiyofumi 愛媛大学, 大学院・医学系研究科, 教授 (00108383)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HATO Naohito 愛媛大学, 大学院・医学系研究科, 准教授 (60284410)
HAKUBA Nobuhiro 愛媛大学, 医学部附属病院, 講師 (70304623)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KAWAGUCHI Akira 東海大学, 医学部・胸部外科, 教授 (30195052)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Keywords | 突発性難聴 / 内耳虚血 / 人工赤血球 / h-LEH / l-LEH / スナネズミ / 有毛細胞 / 活性酸素 / 1-LEH / 一過性内耳虚血 / ABR / 内有毛細胞 / 外有毛細胞 / ナノカプセル / 高酸素親和性 / 低酸素親和性 / 難聴の治療 |
|---|
| Research Abstract |
To test liposome-encapsulated hemoglobin (LEH) in transient cochlear ischemia/reperfusion as a model of sudden deafness, Mongolian gerbils were randomly assigned to receive 2 mL/kg of either low-affinity LEH (l-LEH, P50O2 = 40 mm Hg), high-affinity LEH (h-LEH, P50O2 = 10 mm Hg), homologous red blood cells (RBCs), or saline (each group n = 6) 30 min before 15-min occlusion of the bilateral vertebral arteries and reperfusion. As a result, h-LEH was significantly more protective than l-LEH in suppressing hearing loss, in contrast to RBC or saline treatment. The results suggested that pretreatment with h-LEH is significantly more protective than l-LEH in mitigating hearing loss and underlying pathological damage, in contrast to transfusion or saline infusion.
|
