A research about the similarity with an organization stem cell and the cancer
| Project/Area Number |
22659310
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YOKOO Seiichi 東京大学, 医学部附属病院, 特任研究員 (20345052)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Hideki 癌研究会, 有明病院, 眼科部長 (50282554)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGAMI SATORU 東京大学, 医学部附属病院, 准教授 (10220245)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,260,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | 幹細胞 / 組織幹細胞 / 未分化性 / 角膜輪部シート / 無血清 / 癌 / 体性幹細胞 / 角膜 / 癌幹細胞 |
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| Research Abstract |
It was difficult to obtain the cancer which broke out on the surface of eyes. Therefore I searched the cell line, but there is not the cell line derived from the surface of eyes. Therefore I performed various kinds of examination that should have accumulated data of the normal corneal epithelial stem cell which would become Control Data in the future. I examined the growth factor which an increase effect was reported in to a corneal epithelium. The increase of the corneal epithelium understood(TGFα),(HGF),(KGF) other than EGF that an effect was high. When I used EGF, KGF, TGFα, the manufacture of the corneal epithelium tissue was possible when it for three weeks.
When I make a corneal epithelium-like organization using EGF, there were not undifferentiated candidate marker, cytokeratin 15(CK15) expressed to a cornea limbus epithelium. The corneal epithelium form that multiplied becomes flatness a little in EGF, but, in the corneal epithelium-like organization which manufactured it only by KGF addition, a basal cell is columnar. Therefore expression of undifferentiated marker CK15 which developed in cornea limbus came to be seen in the corneal epithelium-like cell sheet which I made in the culture fluid which added only KGF when I examined undifferentiated marker CK15. The other undifferentiated marker candidates examined, too. As for the corneal epithelium sheet derived from a stem cell cultured using EGF, expression is not seen in them at all. The expression of various undifferentiated markers was seen by addition of KGF including p63, p75NTR, N-Cad. KGF strongly participating in property maintenance of the epithelium stem cell than these results is thought about. Again than a fact of these marker expression, It is thought that I could make a cornea limbus epithelium cell sheet by addition of KGF and am suitable for transplant more.
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Report
(3 results)
Research Products
(54 results)
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[Presentation]2010
Author(s)
大道幸子、臼井智彦、横尾誠一、新家眞、天野史郎
Organizer
第114回日本眼科学会総会
Place of Presentation
名古屋国際会議場名古屋
Year and Date
2010-04-16
Related Report
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[Presentation] Involvement of Leukemia Inhibitory Factor(lif) in Corneal Neovascularization2010
Author(s)
K. Sugisaki, T. Usui, N. Honda, S. Yokoo, S. AmanoK. Sugisaki, T. Usui, N. Honda, S. Yokoo, S. AmanoK. Sugisaki, T. Usui, N. Honda, S. Yokoo, S. Amano
Organizer
ARVO 2010 Annual Meeting
Place of Presentation
Florida, USA
Related Report
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[Presentation] Laminin-5 Enhances Adhesion, Migration, and Proliferation Capacity of Cultured Human Corneal Endothelial Cells2010
Author(s)
M. Yamaguchi, N. Ebihara, N. Shima, M. Kimoto, T. Funaki, S. Yokoo, A. Murakami, S. Yamagami
Organizer
ARVO 2010 Annual Meeting
Place of Presentation
Florida, USA
Related Report
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