| Budget Amount *help |
¥3,260,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Research Abstract |
It was difficult to obtain the cancer which broke out on the surface of eyes. Therefore I searched the cell line, but there is not the cell line derived from the surface of eyes. Therefore I performed various kinds of examination that should have accumulated data of the normal corneal epithelial stem cell which would become Control Data in the future. I examined the growth factor which an increase effect was reported in to a corneal epithelium. The increase of the corneal epithelium understood(TGFα),(HGF),(KGF) other than EGF that an effect was high. When I used EGF, KGF, TGFα, the manufacture of the corneal epithelium tissue was possible when it for three weeks.
When I make a corneal epithelium-like organization using EGF, there were not undifferentiated candidate marker, cytokeratin 15(CK15) expressed to a cornea limbus epithelium. The corneal epithelium form that multiplied becomes flatness a little in EGF, but, in the corneal epithelium-like organization which manufactured it only by KGF addition, a basal cell is columnar. Therefore expression of undifferentiated marker CK15 which developed in cornea limbus came to be seen in the corneal epithelium-like cell sheet which I made in the culture fluid which added only KGF when I examined undifferentiated marker CK15. The other undifferentiated marker candidates examined, too. As for the corneal epithelium sheet derived from a stem cell cultured using EGF, expression is not seen in them at all. The expression of various undifferentiated markers was seen by addition of KGF including p63, p75NTR, N-Cad. KGF strongly participating in property maintenance of the epithelium stem cell than these results is thought about. Again than a fact of these marker expression, It is thought that I could make a cornea limbus epithelium cell sheet by addition of KGF and am suitable for transplant more.
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