The role of autophagy in sepsis and the control over the autophagy-associated pathophysiology
| Project/Area Number |
22659326
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Emergency medicine
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| Research Institution | Chiba University |
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Principal Investigator |
WATANABE Eizo 千葉大学, 大学院・医学研究院, 講師 (40375639)
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| Co-Investigator(Kenkyū-buntansha) |
ODA Shigeto 千葉大学, 大学院・医学研究院, 教授 (90204205)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,340,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | オートファジー / 敗血症 / 細胞死 / 腹膜炎 / 盲腸結紮穿孔 / 集中治療 |
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| Research Abstract |
It is not well understood whether the process of autophagy is accelerated or blocked, and whether it is beneficial or harmful to the immune defense mechanism over a time course during sepsis. Our aim was to determine both the kinetics and the role of autophagy in sepsis. We examined autophagosome and autolysosome formation in a cecal ligation and puncture (CLP) mouse model of sepsis (in C57BL/6N mice and GFP-LC3 transgenic mice), using western blotting, immunofluorescence, and electron microscopy. All autophagy-related processes are activated in a mouse model of early sepsis. Also, inhibition of autophagy process by chloroquine administration immediately after CLP resulted in liver injury and higher mortality; autophagy appears to play a protective role in septic animals.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Mechanisms of cardiac and renal dysfunction in patientsdying of sepsis2013
Author(s)
Takasu O, Gaut JP, Watanabe E, To K, Fagley RE, Sato B, Jarman S, Efimov IR, Janks DL, Srivastava A, Bhayani SB, Drewry A, Swanson PE, Hotchkiss RS
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Journal Title
Am J Respir CritCare Med
Volume: 187
Pages: 509-17
Related Report
Peer Reviewed
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