| Project/Area Number |
22659329
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SHONO Masayuki 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 技術職員 (60380101)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Yasuko 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (40144985)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,150,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | 組織学 / 時空間的定量解析 / 膜輸送蛋白質 / レポーター / ビタミンB12 / ポルフィリン環 / 唾液腺 / ビタミンB12結合蛋白質 / 水チャネル / ピタミンB12結合蛋白質 |
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| Research Abstract |
The green fluorescent protein (GFP) gene is frequently used in spatiotemporal analysis of membrane protein as a reporter gene. GFP gene is introduced into cell using vector systems to trace of a target membrane protein. However, GFP protein is composed of 238 amino acids with the molecular mass of 27kDa. In the target membrane protein with small molecular mass, GFP affects the localization of target membrane protein. It is more convenient that the molecular mass of reporter protein is smaller than that of GFP. We tried to develop to use porphyrin ring of vitamin B12 as a reporter, because porphyrin ring has small molecular mass and fluorescence
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