| Project/Area Number |
22659332
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAKAYAMA Koji 長崎大学, 大学院・医歯薬学総合研究科, 教授 (80150473)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUKUBA Tomoko (KADOWAKI Tomoko) 長崎大学, 大学院・医歯薬学総合研究科, 助教 (70336080)
NAITO Mariko 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (20244072)
SHOJI Mikio 長崎大学, 大学院・医歯薬学総合研究科, 助教 (10336175)
SATO Keiko 長崎大学, 大学院・医歯薬学総合研究科, 助教 (70410579)
YUKITAKE Hideharu 長崎大学, 大学院・医歯薬学総合研究科, 技術職員 (30380984)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,140,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | 遺伝子 / 発現調節 / 二成分制御系 / シグマ因子 / 歯周病 / 歯学 / 細菌 / プロテアーゼ / 膜輸送 |
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| Research Abstract |
We investigated regulatory mechanism of secretion system of pathogenic proteases/ adhesins of the periodontopathogen Porphyromonas gingivalis. Secretion of P. gingivalis gingipain proteases/ adhesins depends on a protein secretion system, Por secretion system(PorSS). We isolated 12 gingipain secretion-deficient mutants and found that two of them were deficient in a two-component system, which is a prokaryotic signaling system and consists of the sensor histidine kinase PorY and response regulator PorX. In this study, we constructed recombinant PorY and PorX proteins and found that PorY was autophosphorylated and the phosphoryl residue of PorY was transferred to PorX. PorY could bind to PorX as revealed by Surface plasmon resonance(BIACORE) analysis. Gene expression of component proteins of the PorSS was also regulated by one of ECF sigma factors, PGN0274, but the PorY-PorX system and the PGN0274 sigma factor independently regulate gene expression of component proteins of the PorSS, suggesting that the PorSS is regulated by multiple regulatory mechanisms.
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