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NETWORK ANALYSIS AMONG HARD TISSUE CELLS USING MULTI-PHOTON MICROSCOPE

Research Project

Project/Area Number 22659335
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionTohoku University

Principal Investigator

WAKAMORI Minoru  東北大学, 大学院・歯学研究科, 教授 (50222401)

Co-Investigator(Renkei-kenkyūsha) NAKAI Jyunichi  埼玉大学, 脳科学癒合研究センター, 教授 (80237198)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,470,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsカルシウム / 機械刺激 / 細胞内 / パッチクランプ法 / カプサイシン受容体 / TRPV1 / チャネル / ネットワーク / ストレッチ
Research Abstract

Osteoblasts and bone cells detect mechanical stimuli, and it is thought that their networks share information. The final goal of this project is to establish the method to analyze the network function among the cells by measuring intracellular Ca^<2+> concentration as an index. Ca^<2+> influx was observed in the osteoblast-like cells after extension. Moreover, Ca^<2+> fluorescent proteins worked like Ca^<2+> indicator fura-2.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (37 results)

All 2013 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (17 results) Book (10 results) Remarks (2 results)

  • [Journal Article] Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6.2012

    • Author(s)
      Unno, T., Wakamori, M., Koike, M., Uchiyama, Y., Ishikawa, K., Kubota, H., Yoshida, T., Sasakawa, H., Peters, C., Mizusawa, H. and Watase K
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 109(43) Issue: 43 Pages: 17693-17698

    • DOI

      10.1073/pnas.1212786109

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rab3-interacting moleculer isoforms lacking the Rab3-binding domain induce long lasting currents but blockneurotransmitter vesicle anchoring in voltage-dependent P/Q-type Ca^<2+> channels2010

    • Author(s)
      Uriu Y, Kiyonaka S, Miki T, Yagi M, Akiyama S, Mori E, Nakao A, Beedle AM, Campbell KP, Wakamori M, Mori Y
    • Journal Title

      J Biol Chem

      Volume: 285 Issue: 28 Pages: 21750-21767

    • DOI

      10.1074/jbc.m110.101311

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A missense mutation of the gene encoding voltage-dependent sodium channel(Nav1. 1) confers susce ptibility to febrile seizures in rats2010

    • Author(s)
      Mashimo T, Ohmori I, Ouchida M, Ohno Y, Tsurumi T, Miki T, Wakamori M, Ishihara S, Yoshida T, Takizawa A, Kato M, Hirabayashi M, Sasa M, Mori Y, Serikawa T
    • Journal Title

      J Neurosci

      Volume: 30(16) Issue: 16 Pages: 5744-5753

    • DOI

      10.1523/jneurosci.3360-09.2010

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neuraonal Ca^<2+>-sensitive non-selective cation channels and TRPC52010

    • Author(s)
      Wakamori M
    • Journal Title

      J Oral Biosci

      Volume: 52 Pages: 352-357

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A missense mutation of the gene encoding voltage-dependent sodium channel(Nav l.l)confers susceptibility to febrile seizures in rats.2010

    • Author(s)
      Mashimo T, Ohmori I, Ouchida M, Ohno Y, Tsurumi T, Miki T, Wakamori M, Ishihara S, Yoshida T, Takizawa A, Kato M, Hirabayashi M, Sasa M, Mori Y, Serikawa T.
    • Journal Title

      J Neurosci

      Volume: 30 Pages: 5744-5753

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rab3-interacting molecule γ isoforms lacking the Rab3-binding domain induce longlasting currents but block neurotransmitter vesicle anchoring in voltage-dependentP/Q-type Ca^<2+>channels.2010

    • Author(s)
      Uriu Y, Kiyonaka S, Miki T, Yagi M, Akiyama S, Mori E, Nakao A, Beedle AM, Campbell KP, Wakamori M, Mori Y.
    • Journal Title

      J Biol Chem

      Volume: 285 Pages: 21750-21767

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuraonal Ca^<2+>-sensitive non-selective cation channels and TRPC5.2010

    • Author(s)
      Wakamori M
    • Journal Title

      J Oral Biosci

      Volume: 52 Pages: 352-357

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 痛覚(侵害受容)とは何か? ユージノールはなぜ効くのか?2010

    • Author(s)
      若森実、近藤大祐、荒木健太郎
    • Journal Title

      日本歯科評論

      Volume: 70 Pages: 135-142

    • Related Report
      2010 Annual Research Report
  • [Presentation] ユージノールのTRPV1チャネルに対する抑制作用2013

    • Author(s)
      吉田卓史、菊池尚、高橋かおり、若森実
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2013-03-21
    • Related Report
      2012 Final Research Report
  • [Presentation] TRP channels and oral sensation2013

    • Author(s)
      Wakamori M
    • Organizer
      Sydney-Tohoku Symposium on Dentistry 2013
    • Place of Presentation
      Sydney、 Australia
    • Year and Date
      2013-01-18
    • Related Report
      2012 Final Research Report
  • [Presentation] フェノール類によるCRACチャネルの遮断2012

    • Author(s)
      鈴木崇弘、坪井明人、吉田卓史、若森実
    • Organizer
      第54回歯科基礎医学会
    • Place of Presentation
      郡山
    • Year and Date
      2012-09-16
    • Related Report
      2012 Final Research Report
  • [Presentation] ユージノールによるTRPV1チャネル活性の抑制作用2012

    • Author(s)
      吉田卓史、高橋かおり、若森実
    • Organizer
      第54回歯科基礎医学会
    • Place of Presentation
      郡山
    • Year and Date
      2012-09-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 鎮静薬ユージノールのTRPV1チャネルに対する効果2012

    • Author(s)
      吉田卓史、菊池尚、高橋かおり、若森実
    • Organizer
      日本薬理学年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 鎮静薬ユージノールのTRPV1チャネルに対する効果2012

    • Author(s)
      吉田卓史、菊池尚、高橋かおり、若森実
    • Organizer
      第83回日本薬理学年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 電位依存性カルシウムチャネルの生物物理学的解析2011

    • Author(s)
      若森実
    • Organizer
      新薬理学セミナー2011
    • Place of Presentation
      仙台
    • Year and Date
      2011-09-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 電位依存性カルシウムチャネルの生物物理学的解析2011

    • Author(s)
      若森実
    • Organizer
      新薬理学セミナー2011
    • Place of Presentation
      仙台(招待講演)
    • Year and Date
      2011-09-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] TRP channels play a role in the mouse osteoblast cell line MC3T3-E1 under shear stress2011

    • Author(s)
      Yoshida T、Wakamori M
    • Organizer
      Annual Meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Yokohama
    • Year and Date
      2011-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] TRP channels play a role in the mouse osteoblast cell line MC3T3-EI under shear stress2011

    • Author(s)
      Yoshida T, Wakamori M
    • Organizer
      The 84^<th> Annual Meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Yokohama
    • Year and Date
      2011-03-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Facilitation of TRPM5 channel activities by fatty acids2011

    • Author(s)
      Wakamori M、Kondoh D、Yoshida T, Kikuchi H、Komatsu M
    • Organizer
      Harvard-Tohoku-Forsyth Symposium
    • Place of Presentation
      Boston、USA
    • Year and Date
      2011-01-06
    • Related Report
      2012 Final Research Report
  • [Presentation] Facilitation of TRPM5 channel activities by fatty acids2011

    • Author(s)
      Wakamori M, Kondoh D, Yoshida T, Kikuchi H, Komatsu M
    • Organizer
      Harvard-Tohoku-Forsyth Symposium
    • Place of Presentation
      Boston, USA
    • Year and Date
      2011-01-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] TRPM5チャネル活性の脂肪酸による修飾2010

    • Author(s)
      近藤大祐、吉田卓史、菊池尚、小松正志、若森実
    • Organizer
      日本薬理学会北部会
    • Place of Presentation
      札幌
    • Year and Date
      2010-09-10
    • Related Report
      2012 Final Research Report
  • [Presentation] TRPM5チャネル活性の脂肪酸による修飾2010

    • Author(s)
      近藤大祐、吉田卓史、菊池尚、小松正志、若森実
    • Organizer
      第61回日本薬理学会北部会
    • Place of Presentation
      札幌
    • Year and Date
      2010-09-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] ユージノールによるTRPV1チャネル活性の抑制作用

    • Author(s)
      吉田卓史、高橋かおり、若森実
    • Organizer
      第54回 歯科基礎医学会
    • Place of Presentation
      郡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] フェノール類によるCRACチャネルの遮断

    • Author(s)
      鈴木崇弘、坪井明人、吉田卓史、若森実
    • Organizer
      第54回 歯科基礎医学会
    • Place of Presentation
      郡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] ユージノールのTRPV1チャネルに対する抑制作用

    • Author(s)
      吉田卓史、高橋かおり、菊地尚、若森実
    • Organizer
      第86回 日本薬理学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Book] 電位依存性チャネル脳神経科学イラストレイテッド2013

    • Author(s)
      若森実、三木崇史、中尾章人、高田宣則、森泰生
    • Publisher
      羊土社
    • Related Report
      2012 Final Research Report
  • [Book] 脳神経科学イラストレイテッド2013

    • Author(s)
      若森 実
    • Publisher
      羊土社
    • Related Report
      2012 Annual Research Report
  • [Book] 歯学英和辞典、監修 渡邉誠2012

    • Author(s)
      若森実(分担執筆、生理学・ 薬理学担当)
    • Publisher
      研究社
    • Related Report
      2012 Final Research Report
  • [Book] 現代歯科薬理学第5版2012

    • Author(s)
      若森実
    • Publisher
      医歯薬出版
    • Related Report
      2012 Final Research Report
  • [Book] 歯学英和辞典(分担執筆、生理学・薬理学担当)2012

    • Author(s)
      若森実
    • Publisher
      研究社
    • Related Report
      2011 Annual Research Report
  • [Book] 現代歯科薬理学2012

    • Author(s)
      若森実
    • Publisher
      医歯薬出版
    • Related Report
      2011 Annual Research Report
  • [Book] Melastatin Transient Receptor Potential Channel Type 5 、 Interface Oral Health Science 20112011

    • Author(s)
      Wakamori M、Yoshida T、Kikuchi T、KondohD 、Komatsu M
    • Publisher
      Springer 社
    • Related Report
      2012 Final Research Report
  • [Book] Interface Oral Health Science 20112011

    • Author(s)
      Wakamori M, Yoshida T, Kikuchi T, Kondoh D, Komatsu M
    • Publisher
      Springer
    • Related Report
      2011 Annual Research Report
  • [Book] 現代歯科薬理学 第5版2011

    • Author(s)
      若森実
    • Publisher
      医歯薬出版 印刷中
    • Related Report
      2010 Annual Research Report
  • [Book] 痛覚(侵害受容)とは何か?ユージノールはなぜ効くのか?2010

    • Author(s)
      若森実、近藤大祐、荒木健太郎
    • Publisher
      日本歯科評論70
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.bsc.tohoku.ac.jp/contents/c2_32/c2_32_wakamori_m.html

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.bsatohoku.ac.jp/contents/c232/c232wakamorim.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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