NETWORK ANALYSIS AMONG HARD TISSUE CELLS USING MULTI-PHOTON MICROSCOPE
| Project/Area Number |
22659335
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
WAKAMORI Minoru 東北大学, 大学院・歯学研究科, 教授 (50222401)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAI Jyunichi 埼玉大学, 脳科学癒合研究センター, 教授 (80237198)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,470,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | カルシウム / 機械刺激 / 細胞内 / パッチクランプ法 / カプサイシン受容体 / TRPV1 / チャネル / ネットワーク / ストレッチ |
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| Research Abstract |
Osteoblasts and bone cells detect mechanical stimuli, and it is thought that their networks share information. The final goal of this project is to establish the method to analyze the network function among the cells by measuring intracellular Ca^<2+> concentration as an index. Ca^<2+> influx was observed in the osteoblast-like cells after extension. Moreover, Ca^<2+> fluorescent proteins worked like Ca^<2+> indicator fura-2.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6.2012
Author(s)
Unno, T., Wakamori, M., Koike, M., Uchiyama, Y., Ishikawa, K., Kubota, H., Yoshida, T., Sasakawa, H., Peters, C., Mizusawa, H. and Watase K
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 109(43)
Issue: 43
Pages: 17693-17698
DOI
Related Report
Peer Reviewed
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[Journal Article] Rab3-interacting moleculer isoforms lacking the Rab3-binding domain induce long lasting currents but blockneurotransmitter vesicle anchoring in voltage-dependent P/Q-type Ca^<2+> channels2010
Author(s)
Uriu Y, Kiyonaka S, Miki T, Yagi M, Akiyama S, Mori E, Nakao A, Beedle AM, Campbell KP, Wakamori M, Mori Y
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Journal Title
J Biol Chem
Volume: 285
Issue: 28
Pages: 21750-21767
DOI
Related Report
Peer Reviewed
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[Journal Article] A missense mutation of the gene encoding voltage-dependent sodium channel(Nav1. 1) confers susce ptibility to febrile seizures in rats2010
Author(s)
Mashimo T, Ohmori I, Ouchida M, Ohno Y, Tsurumi T, Miki T, Wakamori M, Ishihara S, Yoshida T, Takizawa A, Kato M, Hirabayashi M, Sasa M, Mori Y, Serikawa T
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Journal Title
J Neurosci
Volume: 30(16)
Issue: 16
Pages: 5744-5753
DOI
Related Report
Peer Reviewed
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[Journal Article] A missense mutation of the gene encoding voltage-dependent sodium channel(Nav l.l)confers susceptibility to febrile seizures in rats.2010
Author(s)
Mashimo T, Ohmori I, Ouchida M, Ohno Y, Tsurumi T, Miki T, Wakamori M, Ishihara S, Yoshida T, Takizawa A, Kato M, Hirabayashi M, Sasa M, Mori Y, Serikawa T.
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Journal Title
J Neurosci
Volume: 30
Pages: 5744-5753
Related Report
Peer Reviewed
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[Journal Article] Rab3-interacting molecule γ isoforms lacking the Rab3-binding domain induce longlasting currents but block neurotransmitter vesicle anchoring in voltage-dependentP/Q-type Ca^<2+>channels.2010
Author(s)
Uriu Y, Kiyonaka S, Miki T, Yagi M, Akiyama S, Mori E, Nakao A, Beedle AM, Campbell KP, Wakamori M, Mori Y.
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Journal Title
J Biol Chem
Volume: 285
Pages: 21750-21767
Related Report
Peer Reviewed
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