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Role of Wnt-Ror2 signals in ruffled border formation in osteoclasts

Research Project

Project/Area Number 22659339
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionMatsumoto Dental University

Principal Investigator

TAKAHASHI Naoyuki  松本歯科大学, 総合歯科医学研究所, 教授 (90119222)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Yasuhiro  松本歯科大学, 総合歯科医学研究所, 準教授 (20264252)
UEHARA Shunsuke  松本歯科大学, 歯学部, 助教 (90434480)
Co-Investigator(Renkei-kenkyūsha) UDAGAWA Nobuyuki  松本歯科大, 歯学部, 教授 (70245801)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,190,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywords口腔生化学 / 破骨細胞 / Wnt非古典経路 / 極性化 / 波上縁 / Wnt5a-Ror2シグナル / 低分子量Gタンパク質 / Wnt / Rho
Research Abstract

The role of Wnt5a-Ror2 signals in osteoclast function was studied. (1) Bone-resorbing activities of osteoclasts derived from Wnt5a-deficient mice and from Ror2 deficient mice were lower than that of wild-type(WT) osteoclasts. (2) Wnt5a activated Rho, a small G protein, in WT osteoclasts but not in Ror2-deficient osteoclasts. (3) When active Rho was transfected into Ror2-deficient osteoclasts, the bone-resorbing activity was recovered. Thus, Wnt5a-Ror2 signals play important roles in osteoclast function through a Rho-dependent mechanism.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (30 results)

All 2012 2011 2010 Other

All Journal Article (19 results) (of which Peer Reviewed: 19 results) Presentation (11 results)

  • [Journal Article] Regulation of Osteoclast Function2012

    • Author(s)
      Nakamura I, Takahashi N, Jimi E, Udagawa N, Suda T
    • Journal Title

      Mod Rheumatol

      Volume: 22(2) Pages: 167-177

    • NAID

      10030614771

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/21953286

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Carbon nanotubes induce bone calcification by bidirectional interaction with osteoblasts2012

    • Author(s)
      Shimizu M, Kobayashi Y, Mizoguchi T, Nakamura H, Kawahara I, Narita N, Usui Y, Aoki K, Hara K, Haniu H, Ogihara N, Ishigaki N, Nakamura K, Kato H, Kawakubo M, Dohi Y, Taruta S, Kim YA, Endo M, Ozawa H, Udagawa N, Takahashi N, Saito N
    • Journal Title

      Advanced Materials

      Volume: 24 Issue: 16 Pages: 2176-2185

    • DOI

      10.1002/adma.201103832

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Wnt5a-Ror2 signaling between osteoblasts and osteoclast precursors enhances osteoclastogenesis2012

    • Author(s)
      Maeda, K., Kobayashi, Y., Udagawa, N., Uehara, S., Ishihara, A., Mizoguchi, T., Kikuchi, Y., Takada, I., Kato, S., Kani, S., Nishita, M., Marumo, K., Martin, T.J., Minami, Y., Takahashi, N.
    • Journal Title

      Nat.Med.

      Volume: (印刷中) Issue: 3 Pages: 405-12

    • DOI

      10.1038/nm.2653

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tetracyclines convert the osteoclastic-differentiation pathway of orogenitor cells to produce dendritic cell-like cells2012

    • Author(s)
      Kinugawa S, et al
    • Journal Title

      J Immunol

      Volume: 188 Issue: 4 Pages: 1772-1781

    • DOI

      10.4049/jimmunol.1101174

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Daily administration of Eldecalcitol (ED-71), an active vitamin Danalog, increases bone mineral density by suppressing RANKL expression in mouse trabecular bone2012

    • Author(s)
      Harada S, et al
    • Journal Title

      J Bone Miner Res

      Volume: 27 Issue: 2 Pages: 461-473

    • DOI

      10.1002/jbmr.555

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polarized osteoclasts put marks of tartate-resistant acid phosphatase on dentine slices-a simple method for identifying polarized osteoclasts2012

    • Author(s)
      Nakayama T, et al
    • Journal Title

      Bone

      Volume: 49 Issue: 6 Pages: 1331-1339

    • DOI

      10.1016/j.bone.2011.09.045

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased bone mass in mice after a single injection of an anti-RANKL neutralizing antibody : evidence for a bone anabolic effect of PTH in mice with few osteoclasts2011

    • Author(s)
      Furuya Y, et al
    • Journal Title

      J Biol Chem

      Volume: 286 Issue: 42 Pages: 37023-37031

    • DOI

      10.1074/jbc.m111.246280

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hyergravity suppresses bone resorption in ovariectomized rat2011

    • Author(s)
      Ikawa T, et al
    • Journal Title

      Adv Space Res

      Volume: 47 Issue: 7 Pages: 1214-1224

    • DOI

      10.1016/j.asr.2010.12.004

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lineage-Committed Osteoclast Precursors Circulate in Blood and Settle Down Into Bone2011

    • Author(s)
      Muto, A, Mizoguchi, T, Udagawa, N., Ito, S, Kawahara, I, Abiko, Y, Arai, A., Harada, S., Kobayashi, Y., Nakamichi, Y, Penninger, JM, Noguchi, T, Takahashi, N.
    • Journal Title

      Journal of Bone and Mineral Research

      Volume: 26 Issue: 12 Pages: 2978-2990

    • DOI

      10.1002/jbmr.490

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polarized osteoclasts put marks of tartrate-resistant acid phosphatase on dentin slices-A simple method for identifying polarized osteoclasts-2011

    • Author(s)
      Nakayama T, Mizoguchi T, Uehara S, Yamashita T, Kawahara I, Kobayashi Y, Moriyama Y, Kurihara S, Sahara N, Ozawa H, Udagawa N, Takahashi N
    • Journal Title

      Bone

      Volume: 49(6) Pages: 1331-1339

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulatory mechanism of osteoclastogenesis by RANKL and Wnt signals2011

    • Author(s)
      Takahashi N, Maeda K, Ishihara A, Uehara S, Kobayashi Y
    • Journal Title

      Front Biosci

      Volume: 16 Pages: 21-30

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/21196156

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of osteoclasts in culture2011

    • Author(s)
      Udagawa N, et al
    • Journal Title

      Methods Mol Biol

      Volume: 690 Pages: 273-284

    • DOI

      10.1007/978-1-60761-962-8_18

    • ISBN
      9781607619611, 9781607619628
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of cell cycle-arrested quiescent osteoclast precursors in vivo.2010

    • Author(s)
      Takahashi N, et al.
    • Journal Title

      Adv Exp Med Biol

      Volume: 658 Pages: 21-30

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Osteoclastic bone resorption induced by innate immune responses.2010

    • Author(s)
      Koide M, et al.
    • Journal Title

      Periodontol 2000

      Volume: 54 Pages: 235-246

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Function of OPG as a traffic regulator for RANKL is crucial for controlled osteoclastogenesis.2010

    • Author(s)
      Aoki S, et al.
    • Journal Title

      J Bone Miner Res

      Volume: 25 Pages: 1907-1921

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spleen serves as a reservoir of osteoclast precursors through vitamin D-induced IL-34 expression in CSF-1^<op/op> mice

    • Author(s)
      Nakamichi Y, Mizoguchi T, Arai A, Kobayashi Y, Sato M, Penninger JM, Yasuda H, Kato S, DeLuca HF, Suda T, Udagawa N, Takahashi N
    • Journal Title

      Proc Natl Acad Sci UAS

      Volume: (In press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulatory mechanism of osteoclastogenesis by Wnt signaling

    • Author(s)
      Kobayashi Y, Maeda K, Uehara S, Yamashita T, Takahashi N
    • Journal Title

      Inflammation and Regeneration

      Volume: (In press)

    • NAID

      130004943807

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] c-Fos plays an essential role in the up-regulation of RANK expression in osteoclast precursors within the bone microenvironment

    • Author(s)
      Arai A, Mizoguchi T, Harada S, Kobayashi Y, Nakamichi Y, Yasuda H, Penninger JM, Yamada K, Udagawa N, Takahashi N
    • Journal Title

      J Cell Science

      Volume: (In press)

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22454522

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bone Effects of vitamin D-Discrepancies between in vivo and in vitro studies-

    • Author(s)
      Suda T, Takahashi F, Takahashi N
    • Journal Title

      Arch Biochem Biophys

      Volume: (In press)

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22107950

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] Osteoclast precursors in vivo2012

    • Author(s)
      Naoyuki Takahashi
    • Organizer
      東京医科歯科大学GCOE
    • Place of Presentation
      東京医科歯科大学
    • Year and Date
      2012-01-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 分子メカニズムを知る意味とは(招待講演)2011

    • Author(s)
      高橋直之
    • Organizer
      Dentistry Quo Vadis
    • Place of Presentation
      東京
    • Year and Date
      2011-12-11
    • Related Report
      2011 Final Research Report
  • [Presentation] チタンインプラント埋入における生体側のイベント(招待講演)2011

    • Author(s)
      高橋直之
    • Organizer
      Dentistry Quo Vadis
    • Place of Presentation
      東京
    • Year and Date
      2011-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 骨吸収を調節する骨芽細胞の新しい役割(招待講演)2011

    • Author(s)
      高橋直之
    • Organizer
      第26回長崎骨粗鬆症研究会
    • Year and Date
      2011-11-30
    • Related Report
      2011 Final Research Report
  • [Presentation] 骨吸収を調節する骨芽細胞の新しい役割2011

    • Author(s)
      高橋直之
    • Organizer
      第26回長崎骨粗鬆症研究会
    • Place of Presentation
      長崎県医師会館(長崎)(招待講演)
    • Year and Date
      2011-11-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Quiescent Osteoclast Precursors2011

    • Author(s)
      Naoyuki Takahashi
    • Organizer
      Invited lecture 2^<nd> Asia-Pacific Osteoporosis and Bone Meeting
    • Year and Date
      2011-09-07
    • Related Report
      2011 Final Research Report
  • [Presentation] Quiescent Osteoclast Precursors2011

    • Author(s)
      Naoyuki Takahashi
    • Organizer
      2nd Asia-Pacific Osteoporosis and Bone Meeting
    • Place of Presentation
      Gold Coast Convention center Gold Coast, Autraria(招待講演)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 破骨細胞を制御する骨芽細胞の新しい役割(招待講演)2011

    • Author(s)
      高橋直之
    • Organizer
      九州大学大学院講義
    • Place of Presentation
      福岡
    • Year and Date
      2011-05-12
    • Related Report
      2011 Final Research Report
  • [Presentation] 骨吸収の調節機構2011

    • Author(s)
      高橋直之
    • Organizer
      鹿児島大学歯学部セミナー
    • Year and Date
      2011-01-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Noncanonical Wnt signaling and osteoclastogenesis2010

    • Author(s)
      Naoyuki Takahashi
    • Organizer
      22nd Meeting of Korean Society of Bone Metabolism
    • Place of Presentation
      Sheraton Grande Walkerhill, 韓国
    • Year and Date
      2010-11-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] 骨吸収を調節するRANKL-RANK 系2010

    • Author(s)
      高橋直之
    • Organizer
      第28回日本骨代謝会学術集会, ランチョンセミナー
    • Place of Presentation
      京王プラザホテル、東京
    • Year and Date
      2010-07-21
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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