Nano-imaging analyses of intracellular transportation, and intracellular and intranuclear distributions of bioactive material
| Project/Area Number |
22659345
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
HAYASHI Yoshihiko 長崎大学, 大学院・医歯薬学総合研究科, 教授 (20150477)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Shizuka 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (00363458)
FUJIWARA Mamoru 長崎大学, 大学院・医歯薬学総合研究科, 助授 (40336178)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,390,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | ナノテクノロジー / 量子ドット / グルコサミン / 細胞内移行 / 細胞内分布 / 細胞内分布動 |
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| Research Abstract |
The uptake of D-glucosamine hydrochloride into cultured osteoblasts was directly observed using D-glucosamine-conjugated carboxylated quantum dots for efficient labeling of cells. Quantum dots attached at cell surface and were transported into cytoplasm at approximately 3 hours of culture. Furthermore, although the intranuclear distribution was not confirmed, the decrease of quantum dots was recognized in cytoplasm at approximately 7 days of culture.
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Report
(4 results)
Research Products
(23 results)
