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Human tooth hard tissue regeneration by mouse tooth germ

Research Project

Project/Area Number 22659346
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionFukuoka Dental College

Principal Investigator

SAKAGAMI Ryuji  福岡歯科大学, 歯学部, 教授 (50215612)

Co-Investigator(Kenkyū-buntansha) NAGAI Atsushi  福岡歯科大学, 歯学部, 准教授 (70252989)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,280,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords再生医療 / エナメル質 / 歯胚 / 移植・再生医療 / 再生医学 / 歯学 / Sox2 / マイクロアレイ
Research Abstract

Rodents have continuously growing incisors, which tooth germ might be used as a source for enamel regeneration because of its high differentiation and regeneration abilities. In this study, transcriptome analysis was first performed to evaluate the young and old mice tooth germ’s genomic expression, then Sox2 and Oct4 expressions were evaluated immunohistologically and by RT-PCR. The cells derived from the apex of the incisors of GFP mice were transplanted into the subepithelial tissue ofkidney, and hard tissue formation with tooth structure was observed. The method for efficiently collecting the cell source is now under investigation.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (26 results)

All 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 9 results) Presentation (14 results) Remarks (1 results)

  • [Journal Article] Cell viabilities and biodegradation rates of DNA/protamine complexes with two different molecular weights of DNA.2013

    • Author(s)
      Mori, N., Ohno, J., Sakagami, R.,Hayakawa, T., Fukushima, T.
    • Journal Title

      J Biomed Mater Res B Appl Biomater

      Volume: (in press) Issue: 5 Pages: 743-751

    • DOI

      10.1002/jbm.b.32877

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel inhibitory mechanism of nitrogen-containing bisphosphonate on theactivity of Cl- extrusion in osteoclasts.2013

    • Author(s)
      Ohgi, K., Kajiya, H., Okamoto, F., Nagaoka, Y., Onitsuka, T., Nagai, A., Sakagami, R., Okabe, K.
    • Journal Title

      Naunyn Schmiedebergs Arch Pharmacol

      Volume: (in press) Issue: 7 Pages: 589-598

    • DOI

      10.1007/s00210-013-0857-0

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stabilization of MAPO1 by specific binding with folliculin and AMP-activated protein kinase in O6-methylguanine-induced apoptosis.2013

    • Author(s)
      Sano, S., Sakagami, R., Sekiguchi, M., Hidaka, M.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 430 Issue: 2 Pages: 810-815

    • DOI

      10.1016/j.bbrc.2012.11.064

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Activation of AMP-activated protein kinase by MAPO1 and FLCN inducing apoptosis triggered by alkylation mismatch in DNA2012

    • Author(s)
      T.H.Lim, R.Fujikane, S.Sano, R.Sakagami, Y.Nakatsu, T.Tsuzuki, M.Sekiguchi, M.Hidaka
    • Journal Title

      DNA Repair

      Volume: 11 Issue: 3 Pages: 259-266

    • DOI

      10.1016/j.dnarep.2011.11.006

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Activation of AMP-activated protein kinase by MAPO1 and FLCN induces apoptosis triggered by alkylated base mismatch in DNA‥DNA Repair2012

    • Author(s)
      im TH, Fujikane R, Sano S, Sakagami R, Nakatsu Y, Tsuzuki T, Sekiguchi M, Hidaka M
    • Journal Title

      DNA Repair

      Volume: 11 Pages: 259-266

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antibodies against ClC7 inhibit extracellular acidification-induced Cl^-currents and bone resorption activity in mouse osteoclasts2011

    • Author(s)
      Ohgi K, Okamoto F, Kajiya H, Sakagami R, Okabe K
    • Journal Title

      Naunyn Schmiedebergs Arch Pharmacol

      Volume: 383 Issue: 1 Pages: 79-90

    • DOI

      10.1007/s00210-010-0576-8

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cell Viability and Tissue Response of High Molecule Weight DNA/protamine Complex2011

    • Author(s)
      Mori, N., Iwahashi, T., Ohno, J., Shinozaki, Y., Sakagami, R., Mitarai, M., Fukushima, T.
    • Journal Title

      Journal of Oral Tissue Engineering

      Volume: 8 Issue: 3 Pages: 188-194

    • DOI

      10.11223/jarde.8.188

    • NAID

      130004456620

    • ISSN
      1348-9623, 1880-0823
    • Related Report
      2012 Final Research Report
  • [Journal Article] DNA/protamine complex paste for an injectable dental material.2011

    • Author(s)
      Fukushima, T., Ohno, J., Imayoshi, R., Mori, N., Sakagami, R., Mitarai, M., Hayakawa, T.
    • Journal Title

      J Mater Sci Mater Med

      Volume: 22 Issue: 12 Pages: 2607-2615

    • DOI

      10.1007/s10856-011-4446-9

    • Related Report
      2012 Final Research Report
  • [Journal Article] FGF-2 stimulates periodontal regeneration : results of a multi-center randomized clinical trial2011

    • Author(s)
      Kitamura M, Akamatsu M, Machigashira M, 他35名
    • Journal Title

      J Dent Res

      Volume: 90 Issue: 1 Pages: 35-40

    • DOI

      10.1177/0022034510384616

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antibodies against ClC7 inhibit extracellular acidification-induced Cl? currents and bone resorption activity in mouse osteoclasts.2011

    • Author(s)
      Ohgi K, Okamoto F, Kajiya H, Sakagami R, Okabe K
    • Journal Title

      Naunyn Schmiedebergs Arch Pharmacol

      Volume: 383 Pages: 79-90

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] T.Cell Viability and Tissues Response of High Molecule Weight DNA/protamine Complex2011

    • Author(s)
      Mori N, Iwahashi T, Ohno J, Shinozaki Y, Sakagami R, Mitarai M, Fukushima
    • Journal Title

      J Oral Tissue Engineering

      Volume: 8 Pages: 17-24

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Comparison of caspase-14 and filaggrin expression between cornified and non-cornified stratified epithelia.2013

    • Author(s)
      Murakami, H., Okamura K., Hatta, M.,Sakagami, R., Yamazaki, J.
    • Organizer
      第86回日本薬理学会
    • Place of Presentation
      福岡
    • Year and Date
      2013-03-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Regulation of expression of chloride channel accessory protein CLCA in differentiated epithelial cells.2013

    • Author(s)
      Hiromatsu, R., Hatta, M., Ohkubo, T., Sakagami, R., Yamazaki, J.
    • Organizer
      第86回日本薬理学会
    • Place of Presentation
      福岡
    • Year and Date
      2013-03-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Stabilization of MAPO1 during the process of apoptosis induction.2012

    • Author(s)
      Sano, S., Sakagami, R., Sekigichi, M., Hidaka M.
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular mechanism of the induction of apoptosis to suppress mutation and cancer.2012

    • Author(s)
      Hidaka, M., Sano, S., Fujikane, R., Lim, T., Sakagami, R., Nakatsu, Y., Tsuzuki, T., Sekigichi, M.
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-12
    • Related Report
      2012 Final Research Report
  • [Presentation] マウス下顎切歯根尖部の多能性幹細胞にお ける Sox2 の発現2012

    • Author(s)
      丸尾直樹、岡村和彦、谷口邦久、坂上竜資
    • Organizer
      第39回福岡歯科大学学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-11-18
    • Related Report
      2012 Final Research Report
  • [Presentation] ビスフォスフォネ-トの新しい作用点としてのマウス破骨細胞 Clcn7 Cl-輸送体2011

    • Author(s)
      大城希美子, 鍛治屋浩, 岡本富士雄, 堤貴司, 鬼塚得也, 永井淳, 坂上竜資 , 岡部 幸司
    • Organizer
      第89回日本生理学会
    • Place of Presentation
      長野県松本市
    • Year and Date
      2011-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] Comparison of caspase-14 and filaggrin expression between cornified and non-cornified stratified epithelia

    • Author(s)
      Murakami H
    • Organizer
      第86回日本薬理学会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Regulation of expression of chloride channel accessory protein CLCA in differentiated epithelial cells

    • Author(s)
      Hiromatsu R
    • Organizer
      第86回日本薬理学会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Molecular mechanism of the induction of apoptosis to suppress mutation and cancer

    • Author(s)
      Hidaka M
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Stabilization of MAPO1 during the process of apoptosis induction.

    • Author(s)
      Sano S
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Annual Research Report
  • [Presentation] マウス下顎切歯根尖部の多能性幹細胞におけるSox2の発現

    • Author(s)
      丸尾直樹
    • Organizer
      第39回福岡歯科大学学会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 培養上皮細胞におけるCl-チャネル調節因子の遺伝子発現解析

    • Author(s)
      廣松亮
    • Organizer
      第54回歯科基礎医学会
    • Place of Presentation
      郡山市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Epigallocatechin-3-gallate (EGCG) による口腔粘膜の分化に対する影響

    • Author(s)
      村上弘
    • Organizer
      第55回日本歯周病学会
    • Place of Presentation
      札幌市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 窒素含有ビスフォスフォネ-ト (NBPs) は破骨細胞ClC7型Cl-輸送体 (Clcn7) の機能を抑制する

    • Author(s)
      大城希美子
    • Organizer
      第55回日本歯周病学会
    • Place of Presentation
      札幌市
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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