| Project/Area Number |
22659346
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAGAI Atsushi 福岡歯科大学, 歯学部, 准教授 (70252989)
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,280,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | 再生医療 / エナメル質 / 歯胚 / 移植・再生医療 / 再生医学 / 歯学 / Sox2 / マイクロアレイ |
|---|
| Research Abstract |
Rodents have continuously growing incisors, which tooth germ might be used as a source for enamel regeneration because of its high differentiation and regeneration abilities. In this study, transcriptome analysis was first performed to evaluate the young and old mice tooth germ’s genomic expression, then Sox2 and Oct4 expressions were evaluated immunohistologically and by RT-PCR. The cells derived from the apex of the incisors of GFP mice were transplanted into the subepithelial tissue ofkidney, and hard tissue formation with tooth structure was observed. The method for efficiently collecting the cell source is now under investigation.
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