Human tooth hard tissue regeneration by mouse tooth germ

• Project/Area Number: 22659346
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Fukuoka Dental College
• Principal Investigator: SAKAGAMI Ryuji 福岡歯科大学, 歯学部, 教授 (50215612)
• Co-Investigator(Kenkyū-buntansha): NAGAI Atsushi 福岡歯科大学, 歯学部, 准教授 (70252989)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,280,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: 再生医療 / エナメル質 / 歯胚 / 移植・再生医療 / 再生医学 / 歯学 / Sox2 / マイクロアレイ

Research Abstract

Rodents have continuously growing incisors, which tooth germ might be used as a source for enamel regeneration because of its high differentiation and regeneration abilities. In this study, transcriptome analysis was first performed to evaluate the young and old mice tooth germ’s genomic expression, then Sox2 and Oct4 expressions were evaluated immunohistologically and by RT-PCR. The cells derived from the apex of the incisors of GFP mice were transplanted into the subepithelial tissue ofkidney, and hard tissue formation with tooth structure was observed. The method for efficiently collecting the cell source is now under investigation.

Research Products

• [Journal Article] Cell viabilities and biodegradation rates of DNA/protamine complexes with two different molecular weights of DNA. (2013)
  • Author(s): Mori, N., Ohno, J., Sakagami, R.,Hayakawa, T., Fukushima, T.
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: (in press) Issue: 5 Pages: 743-751
  • DOI: 10.1002/jbm.b.32877
  • Peer Reviewed
• [Journal Article] A novel inhibitory mechanism of nitrogen-containing bisphosphonate on theactivity of Cl- extrusion in osteoclasts. (2013)
  • Author(s): Ohgi, K., Kajiya, H., Okamoto, F., Nagaoka, Y., Onitsuka, T., Nagai, A., Sakagami, R., Okabe, K.
  • Journal Title: Naunyn Schmiedebergs Arch Pharmacol Volume: (in press) Issue: 7 Pages: 589-598
  • DOI: 10.1007/s00210-013-0857-0
  • Peer Reviewed
• [Journal Article] Stabilization of MAPO1 by specific binding with folliculin and AMP-activated protein kinase in O6-methylguanine-induced apoptosis. (2013)
  • Author(s): Sano, S., Sakagami, R., Sekiguchi, M., Hidaka, M.
  • Journal Title: Biochem Biophys Res Commun Volume: 430 Issue: 2 Pages: 810-815
  • DOI: 10.1016/j.bbrc.2012.11.064
  • Peer Reviewed
• [Journal Article] Activation of AMP-activated protein kinase by MAPO1 and FLCN inducing apoptosis triggered by alkylation mismatch in DNA (2012)
  • Author(s): T.H.Lim, R.Fujikane, S.Sano, R.Sakagami, Y.Nakatsu, T.Tsuzuki, M.Sekiguchi, M.Hidaka
  • Journal Title: DNA Repair Volume: 11 Issue: 3 Pages: 259-266
  • DOI: 10.1016/j.dnarep.2011.11.006
  • Peer Reviewed
• [Journal Article] Activation of AMP-activated protein kinase by MAPO1 and FLCN induces apoptosis triggered by alkylated base mismatch in DNA‥DNA Repair (2012)
  • Author(s): im TH, Fujikane R, Sano S, Sakagami R, Nakatsu Y, Tsuzuki T, Sekiguchi M, Hidaka M
  • Journal Title: DNA Repair Volume: 11 Pages: 259-266
  • Peer Reviewed
• [Journal Article] Antibodies against ClC7 inhibit extracellular acidification-induced Cl^-currents and bone resorption activity in mouse osteoclasts (2011)
  • Author(s): Ohgi K, Okamoto F, Kajiya H, Sakagami R, Okabe K
  • Journal Title: Naunyn Schmiedebergs Arch Pharmacol Volume: 383 Issue: 1 Pages: 79-90
  • DOI: 10.1007/s00210-010-0576-8
  • Peer Reviewed
• [Journal Article] Cell Viability and Tissue Response of High Molecule Weight DNA/protamine Complex (2011)
  • Author(s): Mori, N., Iwahashi, T., Ohno, J., Shinozaki, Y., Sakagami, R., Mitarai, M., Fukushima, T.
  • Journal Title: Journal of Oral Tissue Engineering Volume: 8 Issue: 3 Pages: 188-194
  • DOI: 10.11223/jarde.8.188
  • NAID: 130004456620
  • ISSN: 1348-9623, 1880-0823
• [Journal Article] DNA/protamine complex paste for an injectable dental material. (2011)
  • Author(s): Fukushima, T., Ohno, J., Imayoshi, R., Mori, N., Sakagami, R., Mitarai, M., Hayakawa, T.
  • Journal Title: J Mater Sci Mater Med Volume: 22 Issue: 12 Pages: 2607-2615
  • DOI: 10.1007/s10856-011-4446-9
• [Journal Article] FGF-2 stimulates periodontal regeneration : results of a multi-center randomized clinical trial (2011)
  • Author(s): Kitamura M, Akamatsu M, Machigashira M, 他35名
  • Journal Title: J Dent Res Volume: 90 Issue: 1 Pages: 35-40
  • DOI: 10.1177/0022034510384616
  • Peer Reviewed
• [Journal Article] Antibodies against ClC7 inhibit extracellular acidification-induced Cl? currents and bone resorption activity in mouse osteoclasts. (2011)
  • Author(s): Ohgi K, Okamoto F, Kajiya H, Sakagami R, Okabe K
  • Journal Title: Naunyn Schmiedebergs Arch Pharmacol Volume: 383 Pages: 79-90
  • Peer Reviewed
• [Journal Article] T.Cell Viability and Tissues Response of High Molecule Weight DNA/protamine Complex (2011)
  • Author(s): Mori N, Iwahashi T, Ohno J, Shinozaki Y, Sakagami R, Mitarai M, Fukushima
  • Journal Title: J Oral Tissue Engineering Volume: 8 Pages: 17-24
  • Peer Reviewed
• [Presentation] Comparison of caspase-14 and filaggrin expression between cornified and non-cornified stratified epithelia. (2013)
  • Author(s): Murakami, H., Okamura K., Hatta, M.,Sakagami, R., Yamazaki, J.
  • Organizer: 第86回日本薬理学会
  • Place of Presentation: 福岡
  • Year and Date: 2013-03-22
• [Presentation] Regulation of expression of chloride channel accessory protein CLCA in differentiated epithelial cells. (2013)
  • Author(s): Hiromatsu, R., Hatta, M., Ohkubo, T., Sakagami, R., Yamazaki, J.
  • Organizer: 第86回日本薬理学会
  • Place of Presentation: 福岡
  • Year and Date: 2013-03-22
• [Presentation] Stabilization of MAPO1 during the process of apoptosis induction. (2012)
  • Author(s): Sano, S., Sakagami, R., Sekigichi, M., Hidaka M.
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-13
• [Presentation] Molecular mechanism of the induction of apoptosis to suppress mutation and cancer. (2012)
  • Author(s): Hidaka, M., Sano, S., Fujikane, R., Lim, T., Sakagami, R., Nakatsu, Y., Tsuzuki, T., Sekigichi, M.
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] マウス下顎切歯根尖部の多能性幹細胞にお ける Sox2 の発現 (2012)
  • Author(s): 丸尾直樹、岡村和彦、谷口邦久、坂上竜資
  • Organizer: 第39回福岡歯科大学学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-11-18
• [Presentation] ビスフォスフォネ-トの新しい作用点としてのマウス破骨細胞 Clcn7 Cl-輸送体 (2011)
  • Author(s): 大城希美子, 鍛治屋浩, 岡本富士雄, 堤貴司, 鬼塚得也, 永井淳, 坂上竜資 , 岡部 幸司
  • Organizer: 第89回日本生理学会
  • Place of Presentation: 長野県松本市
  • Year and Date: 2011-03-30
• [Presentation] Comparison of caspase-14 and filaggrin expression between cornified and non-cornified stratified epithelia
  • Author(s): Murakami H
  • Organizer: 第86回日本薬理学会
  • Place of Presentation: 福岡市
• [Presentation] Regulation of expression of chloride channel accessory protein CLCA in differentiated epithelial cells
  • Author(s): Hiromatsu R
  • Organizer: 第86回日本薬理学会
  • Place of Presentation: 福岡市
• [Presentation] Molecular mechanism of the induction of apoptosis to suppress mutation and cancer
  • Author(s): Hidaka M
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡市
• [Presentation] Stabilization of MAPO1 during the process of apoptosis induction.
  • Author(s): Sano S
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡市
• [Presentation] マウス下顎切歯根尖部の多能性幹細胞におけるSox2の発現
  • Author(s): 丸尾直樹
  • Organizer: 第39回福岡歯科大学学会
  • Place of Presentation: 福岡市
• [Presentation] 培養上皮細胞におけるCl-チャネル調節因子の遺伝子発現解析
  • Author(s): 廣松亮
  • Organizer: 第54回歯科基礎医学会
  • Place of Presentation: 郡山市
• [Presentation] Epigallocatechin-3-gallate (EGCG) による口腔粘膜の分化に対する影響
  • Author(s): 村上弘
  • Organizer: 第55回日本歯周病学会
  • Place of Presentation: 札幌市
• [Presentation] 窒素含有ビスフォスフォネ-ト (NBPs) は破骨細胞ClC7型Cl-輸送体 (Clcn7) の機能を抑制する
  • Author(s): 大城希美子
  • Organizer: 第55回日本歯周病学会
  • Place of Presentation: 札幌市
• [Remarks]