Establisment of differentiation method from induced pluripotent stem(iPS) cells to osteoblasts via mesenchymal stem cells

• Project/Area Number: 22659370
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: MIYAMOTO Youji 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (20200214)
• Co-Investigator(Kenkyū-buntansha): NAGAI Hirokazu 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教 (50282190) ; TAMATANI Tetsuya 徳島大学, 病院, 講師 (30274236) ; UCHIDA Daisuke 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (20335798)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,290,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: iPS細胞 / 骨 / 再生医療 / 間葉系幹細胞 / ハイドロキシアパタイト

Research Abstract

The aim of this study is to establish a method to differentiate iPS cells into mesenchymal stem cells, followed by to differentiate mesenchymal stem cells into osteoblasts, in order to efficiently differentiate from iPS cells into osteoblasts.
The expressions of the markers of mesenchymal stem cells, were enhanced by addition of TGF-βand retinoic acid. Then, we examined the differentiation of the mesenchymal stem cells into osteoblasts by addition of bone induced medium. The expressions of the differentiation markers of osteoblasts were enhanced. In addition, it was revealed that the expression of these differentiation markers of osteoblasts was also increased when the mesenchymal stem cells were cultured on bioceramics, such as hydroxyapatite. Therefore, it was demonstrated that the iPS cells can differentiate into osteoblasts via the mesenchymal stem cells.

Research Products

• [Remarks] ホームページ
  • URL: http://www.dent.tokushima-u.ac.jp.geka2/