Development of basic technology for tooth regeneration using iPS cells.

• Project/Area Number: 22659372
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: TAKANO-YAMAMOTO Teruko 東北大学, 大学院・歯学研究科, 教授 (00127250)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,230,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 再生医療 / ヒトiPS細胞 / ヒト歯髄細胞 / 歯の再生 / iPS細胞 / 人工歯胚

Research Abstract

We planed to generate bioengineered tooth using iPS cells, and have performed fundamental research for it. We established iPS cells derived from human dental pulp cells and, analyzed stem cell marker expression. The results suggest that our iPS cells have features of stem cells.

Research Products

• [Journal Article] Anti-c-Fms antibody inhibits lipopolysaccharide-induced osteoclastogenesis in vivo (2012)
  • Author(s): Kimura K, Kitaura H, Fujii T, Hakami ZW, Takano-Yamamoto T
  • Journal Title: FEMS Immunol Med Microbiol Volume: Vol.64 Pages: 219-227
  • Peer Reviewed
• [Journal Article] Anti-c-Fms antibody inhibits lipopolysaccharide-induced osteoclastogenesis in vivo (2012)
  • Author(s): Kimura K, Takano-Yamamoto T, et al
  • Journal Title: FEMS Immunol Med Microbiol Volume: 64 Pages: 219-227
  • Peer Reviewed
• [Journal Article] Differential roles of CCN family proteins during osteoblast differentiation : Involvement of Smad and MAPK signaling pathway (2011)
  • Author(s): Kawaki H, Kubota S, Suzuki A, Suzuki M, Kohsaka K, Hoshi K, Fujii T, Lazar N, Ohgawara T, Maeda T, Perbal B, Takano-Yamamoto T, Takigawa M
  • Journal Title: Bone Volume: Vol.49 Pages: 975-989
  • Peer Reviewed
• [Journal Article] Functional tooth regeneration using a bioengineered tooth unit as a mature organ replacement regenerative therapy (2011)
  • Author(s): Oshima M, Mizuno M, Imamura A, Ogawa M, Yasukawa M, Yamazaki H, Morita R, Ikeda E, Nakao K, Takano-Yamamoto T, Kasugai S, Saito M, Tsuji T
  • Journal Title: PLos One Volume: Vol.6
  • Peer Reviewed
• [Journal Article] IL-12-and IL-18-mediated, nitric oxide-induced apoptosis in TNF-α-mediated osteoclastogenesis of bone marrow cells (2011)
  • Author(s): Kitaura H, Fujimura Y, Yoshimatsu M, Kohara H, Morita Y, Aonuma T, Fukumoto E, Masuyama R, Yoshida N, Takano-Yamamoto T
  • Journal Title: Calcif Tissue Int Volume: Vol.89 Pages: 65-73
  • Peer Reviewed
• [Journal Article] The three-dimensional morphometry and cell-cell communication of the osteocyte network in chick and mouse embryonic calvaria (2011)
  • Author(s): Sugawara Y, Ando R, Kamioka H, Ishihara Y, Honjo T, Kawanabe N, Kurosaka H, Takano-Yamamoto T, Yamashiro T
  • Journal Title: Calcif Tissue Int Volume: Vol.88 Pages: 416-424
  • Peer Reviewed
• [Journal Article] Fibroblast growth factor 10 regulates Meckel's cartilage formation during early mandibular morphogenesis in rats (2011)
  • Author(s): Terao F, Takahashi I, Mitani H, Haruyama N, Sasano Y, Suzuki O, Takano-Yamamoto T
  • Journal Title: Dev Biol Volume: Vol.350 Pages: 337-347
  • Peer Reviewed
• [Journal Article] Functional tooth regeneration using a bioengineered tooth unit as a mature organ replacement regenerative therapy (2011)
  • Author(s): Ohshima M, Takano-Yamamoto T, et al
  • Journal Title: PLos One Volume: 6
  • Peer Reviewed
• [Journal Article] Differential roles of CCN family proteins during osteoblast differentiation : Involvement of Smad and MAPK signaling pathway (2011)
  • Author(s): Kawaki H, Takano-Yamamoto T, et al
  • Journal Title: Bone Volume: 49 Pages: 975-989
  • Peer Reviewed
• [Journal Article] The three-dimensional morphometry and cell-cell communication of the osteocyte network in chick and mouse embryonic calvaria (2011)
  • Author(s): Sugawara Y, Takano-Yamamoto T, et al
  • Journal Title: Calcif Tissue Int Volume: 88 Pages: 416-424
  • Peer Reviewed
• [Journal Article] IL-12- and IL-18-mediated, nitric oxide-induced apoptosis in TNF-α-mediated osteoclastogenesis of bone marrow cells (2011)
  • Author(s): Kitaura H, Takano-Yamamoto T, et al
  • Journal Title: Calcif Tissue Int Volume: 89 Pages: 65-73
  • Peer Reviewed
• [Journal Article] Fibroblast growth factor 10 regulates Meckel's cartilage formation duringearly mandibular morphogenesis in rats (2011)
  • Author(s): Fumie Terao
  • Journal Title: Developmental Biology Volume: 15 Pages: 337-347
  • Peer Reviewed
• [Journal Article] Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice (2010)
  • Author(s): Murakami T, Fukunaga T, Takeshita N, Hiratsuka K, Abiko Y, Yamashiro T, Takano-Yamamoto T
  • Journal Title: J Anat Volume: Vol.217 Pages: 236-244
  • Peer Reviewed
• [Journal Article] Effects of CO2 laser irradiation of the gingiva during tooth movement (2010)
  • Author(s): Seiryu M, Deguchi T, Fujiyama K, Sakai Y, Daimaruya T, Takano-Yamamoto T
  • Journal Title: J Dent Res Volume: Vol.89 Pages: 537-542
  • Peer Reviewed
• [Journal Article] Effects of CO_2 laser irradiation of the gingiva during tooth movement (2010)
  • Author(s): Masahiro Seiryu
  • Journal Title: Journal of Dental Research Volume: 89 Pages: 537-542
  • Peer Reviewed
• [Journal Article] Isolation of multipotent stem cells in human periodontal ligament usingstage specific embryonic antigen-4 (2010)
  • Author(s): Noriaki Kawanabe
  • Journal Title: Differentiation Volume: 79 Pages: 74-83
  • Peer Reviewed
• [Journal Article] Dynamic expression of Six family genes in the dental mesenchyme andthe epithelial ameloblast stem/progenitor cells during murine tooth development (2010)
  • Author(s): Kouji Nonomura
  • Journal Title: Journal of Anatomy Volume: 216 Pages: 80-91
  • Peer Reviewed
• [Journal Article] Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice (2010)
  • Author(s): Takashi Murakami
  • Journal Title: Journal of Anatomy Volume: 217 Pages: 236-244
  • Peer Reviewed
• [Presentation] 矯正的歯の移動モデルを用いた歯根膜幹細胞ニッチの解析 (2012)
  • Author(s): 山本照子
  • Organizer: 平成23年度共同研究会「再生医学・再生医療の先端融合的共同研究拠点」(招待講演)
  • Place of Presentation: 京都
  • Year and Date: 2012-03-12
• [Presentation] 矯正的歯の移動モデルを用いた歯根膜幹細胞ニッチの解析 (2012)
  • Author(s): 山本照子
  • Organizer: 平成23年度共同研究会「再生医学・再生医療の先端融合的共同研究拠点」
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2012-03-12
• [Presentation] Orthodontic treatment using mini-screw anchorage for patients with temporomandibular joint dysfunction (2011)
  • Author(s): Takano-Yamamoto T
  • Organizer: 2nd World Implant Orthodontic Congress (WIOC)(招待講演)
  • Place of Presentation: Taipei
  • Year and Date: 2011-12-11
• [Presentation] 骨芽細胞分化におけるCCNファミリータンパク質の分布と機能解析 (2011)
  • Author(s): 川木晴美、山本照子, 他
  • Organizer: 第53回歯科基礎医学会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-09-30
• [Presentation] CCNファミリータンパク質によるMAPK経路を介した骨芽細胞増殖・分化調節 (2011)
  • Author(s): 川木晴美、山本照子, 他
  • Organizer: 第4回日本CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2011-08-27
• [Presentation] FGF10 contributes Meckel's cartilage formation in early mandibular development (2011)
  • Author(s): Terao F, Takano-Yamamoto T, et al.
  • Organizer: The 6th International Workshop on Mano-, Bio-and Amorphous Materials
  • Place of Presentation: Togatta
  • Year and Date: 2011-08-09
• [Presentation] FGF10 contributes Meckel's cartilage formation in early mandibular development (2011)
  • Author(s): Terao F, Takano-Yamamoto T, et al
  • Organizer: The 6th International Workshop on Mano-, Bio- and Amorphous Materials
  • Place of Presentation: 遠刈田
  • Year and Date: 2011-08-09
• [Presentation] S-アデノシルメチオニン合成酵素MATIIによるエピゲノム制御と転写調節 (2011)
  • Author(s): 解良洋平、山本照子, 他
  • Organizer: 第77回日本生化学会東北支部例会
  • Place of Presentation: 仙台
  • Year and Date: 2011-07-23
• [Presentation] 再生歯による移植システムの開発(1)ー再生歯ユニットの作製と成体顎骨への生着の解析ー (2011)
  • Author(s): 水野光政、山本照子, 他
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02
• [Presentation] 再生歯による移植システムの開発(1)ー再生歯ユニットによる歯槽骨と歯の生理機能の回復 (2011)
  • Author(s): 大島正充、山本照子, 他
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02
• [Presentation] 再生歯による移植システムの開発(1)-再生歯ユニットの作製と成体顎骨への生着の解析- (2011)
  • Author(s): 水野光政
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02
• [Presentation] 再生歯による移植システムの開発(2)一再生歯ユニットによる歯槽骨と歯の生理機能の回復 (2011)
  • Author(s): 大島正充
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02
• [Presentation] Methionine adenosyltransferase (MATII)-dependent writing of histone H3K9 methylation of Cox2 gene (2011)
  • Author(s): 解良洋平、山本照子, 他
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] なぜ歯は動くのか?-矯正的歯の移動の分子メカニズム- (2011)
  • Author(s): 山本照子
  • Organizer: 第70回日本矯正歯科学会大会(招待講演)
  • Place of Presentation: 名古屋
• [Presentation] 再生歯ユニット移植による歯の生理的機能の再生 (2011)
  • Author(s): 大島正充、池田悦子、山本照子, 他
  • Organizer: 第70回日本矯正歯科学会大会
  • Place of Presentation: 名古屋
• [Presentation] 再生歯ユニットの作製と生体顎骨への生着解析 (2011)
  • Author(s): 水野光政、池田悦子、山本照子, 他
  • Organizer: 第70回日本矯正歯科学会大会
  • Place of Presentation: 名古屋
• [Presentation] IL-12-and IL-18-mediated nitric oxide-induced apoptosis in TNF-α-mediated osteoclastogenesis. (2011)
  • Author(s): Kitaura H, Takano-Yamamoto T, et al
  • Organizer: The 4th International Congress and the 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Nagoya
• [Presentation] Anti-c-Fms antibody inhibits lipopolysaccharide-induced osteoclastogenesis in vivo (2011)
  • Author(s): Kimura K, Takano-Yamamoto T, et al.
  • Organizer: The 4th International Congress and the 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Nagoya
• [Presentation] Interleukin-4 inhibits tumor necrosis factor-α-mediated osteoclast formation in vivo. (2011)
  • Author(s): Fujii T, Takano-Yamamoto T, et al.
  • Organizer: The 4th International Congress and the 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Nagoya
• [Presentation] 胎生期下顎形成期におけるFGF10によるメッケル軟骨形態の制御 (2011)
  • Author(s): 寺尾文恵、山本照子, 他
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] なぜ歯は動くのか?-矯正的歯の移動の分子メカニズム- (2011)
  • Author(s): 山本照子
  • Organizer: 第70回日本矯正歯科学会大会
  • Place of Presentation: 名古屋(招待講演)
• [Presentation] Interleukin-4 inhibits tumor necrosis factor-α-mediated osteoclast formation in vivo (2011)
  • Author(s): Fujii T, Takano-Yamamoto T, et al
  • Organizer: The 4th International Congress and the 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: 名古屋
• [Presentation] Anti-c-Fms antibody inhibits lipopolysaccharide-induced osteoclastogenesis in vivo. marrow cells in TNF-α-induced osteoclast formation (2011)
  • Author(s): Kimura K, Takano-Yamamoto T, et al
  • Organizer: The 4th International Congress and the 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: 名古屋
• [Presentation] IL-12- and IL-18-mediated nitric oxide-induced apoptosis in TNF-α-mediated osteoclastogenesis (2011)
  • Author(s): Kitaura H, Takano-Yamamoto, et al
  • Organizer: The 4th International Congress and the 70th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: 名古屋
• [Presentation] Orthodontic treatment using mini-screw anchorage for patients with temporomandibular joint dysfunction. (2010)
  • Author(s): Takano-Yamamoto T
  • Organizer: 2^<nd> World Implant Orthodontic Congress(WIOC)
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2010-12-11
• [Presentation] 歯の機能的な再生(I):再生歯ユニットの作製と成体顎骨への生着の解析 (2010)
  • Author(s): 大島正充、山本照子, 他
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 歯の機能的な再生(II):再生歯ユニット移植による歯の生理的機能の再生 (2010)
  • Author(s): 今村彩、山本照子, 他
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Orthodontic treatment using mini-screw anchorage for patients with temporomandibular joint dysfunction (2010)
  • Author(s): Takano-Yamamoto T
  • Organizer: 3rd Asian Micro-Implant Anchorage Congress
  • Place of Presentation: Bangkok
  • Year and Date: 2010-11-12
• [Presentation] Orthodontic treatment using mini-screw anchorage for patients with temporomandibular joint dysfunction. (2010)
  • Author(s): Takano-Yamamoto T
  • Organizer: 3^<rd> Asian Micro-lmplant Anchorage Congress
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2010-11-12
• [Presentation] 機能的な歯の再生(I):機能的な歯の構造体:再生歯ユニットの作製技術の開発 (2010)
  • Author(s): 水野光政、山本照子, 他
  • Organizer: 第8回日本再生歯科医学会学術大会・総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-10-30
• [Presentation] 機能的な歯の再生(II):再生歯ユニット移植による生体顎骨への生着と歯槽骨再生 (2010)
  • Author(s): 大島正充、山本照子, 他
  • Organizer: 第8回日本再生歯科医学会学術大会・総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-10-30
• [Presentation] 機能的な歯の再生(III):再生歯ユニット移植による歯の生理的機能の再生 (2010)
  • Author(s): 山崎大道、山本照子, 他
  • Organizer: 第8回日本再生歯科医学会学術大会・総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-10-30