Profiling of microRNAs in mouse mandibular condylar cartilage during development

• Project/Area Number: 22659376
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Kyushu University
• Principal Investigator: TAKAHASHI Ichiro 九州大学, 歯学研究院, 教授 (70241643)
• Co-Investigator(Kenkyū-buntansha): TERAO Fumie 九州大学, 大学院・歯学研究院, 助教 (10510018)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: microRNA / 下顎頭軟骨 / 歯科矯正学 / 軟骨細胞分化 / 細胞・組織 / 軟骨細胞 / 下顎頭 / 遺伝子発現制御

Research Abstract

We focused on miR-200a by microarray analysis of developing mandibular condylar cartilage (MCC) in mouse. Transfection experiments of miR-200a inhibitor or mimic were performed and demonstrated that miR-200a contributes to chondrogenesis in developing MCC through the control of proliferation and differentiation in MCC cells

Research Products

• [Journal Article] Three-dimentional analysis of the pharyngeal airway morphology in growing Japanese females with and without cleft lip and palate. (2012)
  • Author(s): Yoshihara M, Terajima M, Yanagita N, Hyakutake H, Kanomi R, Kitahara T, Takahashi I.
  • Journal Title: Am J Orthod Dentofacial Orthopedics Volume: 141(4)
  • Peer Reviewed
• [Journal Article] Fabrication of carbonate apatite block from calcium sulfate by hydrothermal treatment (2012)
  • Author(s): Nomura S, Tsuru K, Valanezhad A, Matsuya S, Takahashi I, Ishikawa K.
  • Journal Title: Key Engineering Materials
  • Peer Reviewed
• [Journal Article] Fabrication of carbonate apatite block from calcium sulfate by hydrothermal treatment (2012)
  • Author(s): Nomura S, Tsuru K, Valanezhad A, Matsuya S, Takahashi I, Ishikawa K
  • Journal Title: Key Engineering Materials Volume: 493-494 Pages: 139-142
  • DOI: 10.4028/www.scientific.net/kem.493-494.139
  • Peer Reviewed
• [Journal Article] Effects of buccal corridors on smile esthetics in Japanese and Korean orthodontists and orthodontic patients. (2012)
  • Author(s): Ioi H, Kang S, Shimomura T, Kim SS, Park SB, Son WS, Takahashi I.
  • Journal Title: Am J Orthod Dentofacial Orthop Volume: 142 Issue: 4 Pages: 459-465
  • DOI: 10.1016/j.ajodo.2012.05.011
  • Peer Reviewed
• [Journal Article] 上下顎臼圧下によるvertical controlにより骨格的開咬の改善を予知的に行なった成人開咬症例 (2012)
  • Author(s): 因絵美子、高橋一郎
  • Journal Title: 九矯歯誌 Volume: 8 Pages: 35-44
  • Peer Reviewed
• [Journal Article] 鋏状咬合および顎関節症を伴うskeletal Class I 叢生症例―矯正治療過程における顎関節疼痛の変化について (2012)
  • Author(s): 辻恭子、高橋一郎
  • Journal Title: 九矯歯誌 Volume: 8 Pages: 45-52
  • Peer Reviewed
• [Journal Article] 上顎両側犬歯の水平埋伏を伴うAngel Class II症例 (2012)
  • Author(s): 柳田奈津美、寺嶋雅彦、高橋一郎
  • Journal Title: 九矯歯誌 Volume: 8 Pages: 69-76
  • Peer Reviewed
• [Journal Article] Effects of retraction of anterior teeth and initial softtissue variables on lip changes in Japanese adults (2012)
  • Author(s): Hayashida H., et al
  • Journal Title: Eur J Orthod Volume: 33(4) Issue: 4 Pages: 419-426
  • DOI: 10.1093/ejo/cjq095
  • Peer Reviewed
• [Journal Article] Three-dimensional analysis of the pharyngeal airway morphology in growing Japanese females with and without cleft lip and palate (2012)
  • Author(s): Yoshihara M., et al
  • Journal Title: Am J Orthod Dentofacial Orthopedics Volume: (in press)
  • Peer Reviewed
• [Journal Article] Comparison of orthodontic treatment outcomes in adults with skeletal open bite between conventional edgewise treatment and implant-anchored orthodontics. (2011)
  • Author(s): Deguchi T, Kurosaki H, Oikawa H, Kuroda S, Takahashi I, Yamashiro T, Takano-Yamamoto T.
  • Journal Title: Am J Orthod Dentofacial Orthopedics Volume: 139(4)
  • Peer Reviewed
• [Journal Article] The primary site of the acrocephalic feature in Apert syndrome is a dwarf cranial base with accelerated chondrocytic differentiation due to aberrant activation of the FGFR2 signaling (2011)
  • Author(s): Nagata M, GH.Nuckolls, X Wang, L Shum, Seki Y, Kawase T, Takahashi K, Nonaka K, Takahashi I, AA.Nomar, Suzuki K, SlavkinHC.
  • Journal Title: Bone Volume: 48 Pages: 847-856
  • Peer Reviewed
• [Journal Article] Effects of retraction of anterior teeth and initial soft tissue variables on lip changes in Japanese adults (2011)
  • Author(s): Hayashida H, Ioi H, Nakata S, Takahashi I, Counts AL
  • Journal Title: Eur J Orthod Volume: 33(4) Pages: 419-26
  • Peer Reviewed
• [Journal Article] Involvement of PRIP, phospholipase C-related, but catalytically inactive protein, in bone formation (2011)
  • Author(s): Tsutsumi K, Matsuda M, Kotani M, Mizokami A, Murakami A, Takahashi I, Terada Y, Kanematsu T, Fukami K, Takenawa T, Jimi E, Hirata M.
  • Journal Title: J Biol Chem Volume: 286(35) Pages: 31032-42
  • Peer Reviewed
• [Journal Article] Three-dimensional analyisis of the temporomandibular joint and fossa-condyle relationship. (2011)
  • Author(s): Endo M, Terajima M, Goto T, Tokumori K, Takahashi I
  • Journal Title: Orthodontics Volume: 12(3) Pages: 210-221
  • Peer Reviewed
• [Journal Article] Comparison of orthodontic treatment outcomes in adults with skeletal open bite between conventional edgewise treatment and implant-anchored orthodontics (2011)
  • Author(s): Deguchi T., et al
  • Journal Title: Am J Orthod Dentofacial Orthopedics Volume: 139(4) Issue: 4 Pages: 60-68
  • DOI: 10.1016/j.ajodo.2009.04.029
  • Peer Reviewed
• [Journal Article] The primary site of the acrocephalic feature in Apert syndrome is a dwarf cranical base with accelerated chondrocytic differentiation due to aberrant activation of the FGFR2 signaling (2011)
  • Author(s): Nagata M., et al
  • Journal Title: Bone Volume: 48 Issue: 4 Pages: 847-856
  • DOI: 10.1016/j.bone.2010.11.014
  • Peer Reviewed
• [Journal Article] Three-dimensional analysis of the temporomandibular joint and fossa-condyle relationship (2011)
  • Author(s): Endo M., et al
  • Journal Title: Orthodontics Volume: 12(3) Pages: 210-221
  • Peer Reviewed
• [Journal Article] Involvement of PRIP, Phospholipase C-related, but Catalytically Inactive Protein, in Bone Formation (2011)
  • Author(s): Koshiro Tsutsumi
  • Journal Title: Journal of Biological Chemistry Volume: 286 Issue: 35 Pages: 31032-31042
  • DOI: 10.1074/jbc.m111.235903
  • Peer Reviewed
• [Journal Article] Fibroblast Growth Factor 10 Regulates Meckel's Cartilage Formationduring Early Mandibular Morphogenesis in Rats. (2011)
  • Author(s): Terao F, Takahashi I, 他
  • Journal Title: Developmental Biology Volume: 350 Pages: 337-347
  • Peer Reviewed
• [Journal Article] Expression of collagen type 1 and 2 on articular cartilage in a rat knee contracture model (2010)
  • Author(s): Hagiwara Y, Ando A, Chimoto E, Tsuchiya M, Takahashi I, Sasano Y, Onoda Y, Suda H, Itoi E
  • Journal Title: Connective Tissue Research Volume: 51 Pages: 22-30
  • Peer Reviewed
• [Journal Article] Induction of MMP-2 at the interface between epithelial cells and fibroblasts from human periodontal ligament (2010)
  • Author(s): Shimonishi M, Takahashi I, Terao F, Komatsu M, Kikuchi M.
  • Journal Title: J Periodont Res Volume: 45 Pages: 309-316
  • Peer Reviewed
• [Journal Article] Expression of collagen type 1 and 2 on articular cartilage in a rat knee contracture model. (2010)
  • Author(s): Hagiwara H, Takhashi I, 他
  • Journal Title: Connective Tissue Research Volume: 51 Pages: 22-30
  • Peer Reviewed
• [Presentation] Real-time Monitoring of Intracellular ERK in ATDC5 Under Mechanical Stress (2013)
  • Author(s): Terao F, Umeda M, Yoshizaki K, Takahashi I
  • Organizer: General Session of International Association for Dental Research
  • Place of Presentation: シアトル・アメリカ合衆国
  • Year and Date: 2013-03-23
• [Presentation] Profiling MicroRNA Expression in Mouse Mandibular Condylar Cartilage during Development (2013)
  • Author(s): Umeda M, Terao F, Yoshizaki K, Takahashi I
  • Organizer: General Session of International Association for Dental Research
  • Place of Presentation: シアトル・アメリカ合衆国
  • Year and Date: 2013-03-21
• [Presentation] Profiling MicroRNA Expression in Mouse Mandibular Condylar Cartilage during Development (2013)
  • Author(s): Umeda M
  • Organizer: General Session of International Association for Dental Research
  • Place of Presentation: シアトル, アメリカ合衆国
• [Presentation] Real-time Monitoring of Intracellular ERK in ATDC5 Under Mechanical Stress (2013)
  • Author(s): Terao F
  • Organizer: General Session of International Association for Dental Research
  • Place of Presentation: シアトル, アメリカ合衆国
• [Presentation] Fabrication of Carbonate Apatite Block from Calcium Sulfate by Hydrothermal Treatment (2011)
  • Author(s): Nomura S, Tsuru K, Valanezhad A, Matsuya S, Takahashi I, Ishikawa K
  • Organizer: BIOCERAMICS23
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2011-11-07
• [Presentation] Fabrication of Carbonate Apatite Block from Calcium Sulfate by Hydrothermal Treatment (2011)
  • Author(s): Nomura S
  • Organizer: BIOCERAMICS 23
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2011-07-11
• [Presentation] Effects of buccalcorridors on smile esthetics in Korean and Japanese orthodontic patients. (2011)
  • Author(s): Ioi H, Kang S, Shimomura T, Kim S, Park S, Son W, Takahashi I, Counts AL
  • Organizer: American Association of Orthodontists
  • Place of Presentation: Chicago,IL
  • Year and Date: 2011-05-14
• [Presentation] Effects of buccal corridors on smile esthetics in Korean and Japanese orthodontic patients (2011)
  • Author(s): Ioi H
  • Organizer: American Association of Orthodontists
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-14
• [Presentation] Expression pattern and possible function of thymosin beta-10 in developing tooth gern compared with thymosin bata-4. (2011)
  • Author(s): Shiotsuka M, Wada H, Kiyoshima T, Kobayashi I, Nagata K, Fujiwara H, Takahashi I, Sakai H.
  • Organizer: The 6^<th> International Joint Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration" and "Oral Health Science"
  • Place of Presentation: 福岡市
  • Year and Date: 2011-03-04
• [Presentation] Effects of allergic inflammation on orthodontically induced tooth root resorption. (2011)
  • Author(s): Murata N, Ioi H, Ohuchi M, Takao T, Oida H, Yamaza T, Takahashi I, Kido MA.
  • Organizer: The 6^<th> International Joint Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration" and "Oral Health Science"
  • Place of Presentation: 福岡市
  • Year and Date: 2011-03-04
• [Presentation] Expression pattern and possible function of thymosin beta-10 in developing tooth gern compared with thymosin bata-4. (2011)
  • Author(s): Shiotsuka M, Wada H, Kiyoshima T, Kobayashi I, Nagata K, Fujiwara H, Takahashi I, Sakai H.
  • Organizer: The 6thInternational Joint Symposium on " Dental and Craniofacial Morphogenesis and Tissue Regeneration " and " Oral Health Science"(第6回国際シンポジウム)
  • Place of Presentation: Fukuoka.
• [Presentation] Effects of allergic inflammation on orthodontically induced tooth root resorption (2011)
  • Author(s): Murata N, Ioi H, Ohuchi M, Takao T, Oida H, Yamaza T, Takahashi I, Kido MA.
  • Organizer: The 6thInternational Joint Symposium on " Dental and Craniofacial Morphogenesis and Tissue Regeneration " and " Oral Health Science"(第6回国際シンポジウム)
  • Place of Presentation: Fukuoka.
• [Presentation] 胎生期下顎形成期における FGF10 によるメッケル軟骨形態の制御 (2011)
  • Author(s): 寺尾文恵、高橋一郎、三谷英稔、春山直人、笹野泰之、鈴木治、山本照子
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪市
• [Presentation] FGF10 contributes Meckel's cartilage formation in early mandibular development. (2011)
  • Author(s): Terao F.
  • Organizer: The 6thInternational Workshop on Nano-,Bioand Amorphous Materials
  • Place of Presentation: ao, Miyagi
• [Presentation] FGF10 contributes Meckel's cartilage formation in early mandibular development (2011)
  • Author(s): Terao F
  • Organizer: he 6th International Workshop on Nano-, Bio- and Amorphous Materials
  • Place of Presentation: Zao, Miyagi
• [Presentation] 歯胚におけるphosphoglycerate kinase(Pgk)1タンパクの局在とその新しい役割について (2010)
  • Author(s): 本田淳也, 小林家吉, 坂井英隆, 高橋一郎
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜市
  • Year and Date: 2010-09-27
• [Presentation] Mechano-Biology of cytoskeletal reorganization in differentiating chondrocytes. (2010)
  • Author(s): Takahashi I
  • Organizer: International Workshop on Nano, Bio and Amorphous Materials
  • Place of Presentation: 宮城、蔵王
  • Year and Date: 2010-08-09
• [Presentation] Lip-morphological differences between class and class molocclusion before/after orthodontic treatment.. (2010)
  • Author(s): Ialam R, Kitahara T, Naher L, Takahashi I
  • Organizer: International Association for Dental Research
  • Place of Presentation: Spain, Barcelona
  • Year and Date: 2010-07-15
• [Presentation] Influence of gingival display on smile esthetics in Japanese. (2010)
  • Author(s): Ioi H, Shimomura T, Nakata S, Takahashi I, Counts AL.
  • Organizer: 110^<th> annual session of American Association of Orthodontists
  • Place of Presentation: USA, Washington
  • Year and Date: 2010-05-02
• [Presentation] カルシウム塩導入による炭酸アパタイト骨補填材の機械的強さ向上 (2010)
  • Author(s): 松元歌奈子, 都留寛治, 川内義一郎, 丸田道人, 松家茂樹, 高橋一郎, 石川邦夫
  • Organizer: 第55回日本歯科理工学会学術講演会
  • Place of Presentation: 東京都
  • Year and Date: 2010-04-17
• [Presentation] Mechano-Biology of cytoskeletal reorganization in differentiating chondrocytes. International Workshop on Nano (2010)
  • Author(s): Takahashi I
  • Organizer: Bio and Amorphous Materials
  • Place of Presentation: Miyagi-Zao
• [Presentation] 歯胚における phosphoglycerate kinase(Pgk)1 タンパクの局在とその新しい役割について. (2010)
  • Author(s): 本田淳也,小林家吉,坂井英隆,高橋一郎
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜市
• [Book] 東北矯正歯科学会雑誌第20巻1号 口腔顎顔面成長発育の生物学-細胞外基質代謝と機械的刺激応答- (2012)
  • Author(s): 高橋一郎
• [Book] 2012 歯科医療秋号 顎顔面の成長発育-下顎頭軟骨の成長メカニズム
  • Author(s): 高橋一郎、梅田まりこ、寺尾文恵
  • Total Pages: 15
• [Remarks]
  • URL: http://www.ortho.dent.kyushu-u.ac.jp/
• [Remarks]
  • URL: http://www.ortho.dent.kyushu-u.ac.jp/