Chromatin regulation and nuclear architecture during activity-dependent transcription in post-mitotic neurons

• Project/Area Number: 22680027
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Gunma University (2011-2012); Nara Institute of Science and Technology (2010)
• Principal Investigator: TAKIZAWA Takumi 群馬大学, 大学院・医学系研究科, 准教授 (30531115)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000); Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2010: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
• Keywords: 分子・細胞神経科学 / 細胞核構造 / ニューロン / クロマチン / 転写 / 神経活動依存性転写 / 核構造 / エピジェネティクス

Research Abstract

We have studied the transcriptional regulation of activity dependent genes from the aspects of chromatin dynamics and spatial gene positioning. We have revealed, in this study, that temporal regulation of activity dependent genes is associated with their spatial positioning. We also found that chromatin-related proteins occupying activity-dependent genes dynamically change their behavior in a neuronal activity dependent manner.

Research Products

• [Journal Article] Chromatin accessibility at a STAT3 target site is altered prior to astrocyte differentiation (2013)
  • Author(s): Satoshi Urayama, Katsunori Semi, Tsukasa Sanosak1, Yukina Hori, Masakazu Namihira, Jun Kohyama, Takumi Takizawa, Kinichi Nakashima
  • Journal Title: Cell Struct Funct
  • NAID: 130004053894
  • Peer Reviewed
• [Journal Article] Meshorer, E.Chromatin immunoprecipitation in mouse hippocampal cells and tissues (2012)
  • Author(s): Sailaja, BS., Takizawa, T.
  • Journal Title: Methods Mol Biol Volume: 809 Pages: 353-64
  • Peer Reviewed
• [Journal Article] Chromatin immunoprecipitation in mouse hippocampal cells and tissues (2012)
  • Author(s): Sailaja BS, Takizawa T, Meshorer E
  • Journal Title: Methods Mol Biol Volume: 809 Pages: 353-64
  • DOI: 10.1007/978-1-61779-376-9_24
  • ISBN: 9781617793752, 9781617793769
  • Peer Reviewed
• [Journal Article] Role of GLI2 in the growth of human osteosarcoma (2011)
  • Author(s): Nagao, H., Ijiri, K., Hirotsu, M., Ishidou, Y., Yamamoto, T., Nagano, S., Takizawa, T., Nakashima, K., Komiya, S. & Setoguchi, T.
  • Journal Title: J athol Volume: 224 Pages: 169-179
  • Peer Reviewed
• [Presentation] 神経系細胞におけるクロマチン空間配置 (2013)
  • Author(s): 滝沢琢己
  • Organizer: 第7回茨城大学遺伝子実験施設公開シンポジウム
  • Place of Presentation: 茨城県
  • Year and Date: 2013-03-11
• [Presentation] 神経幹細胞における遺伝子座核内配置と転写活性の関連 (2012)
  • Author(s): 伊藤謙治、魚崎祐一、野口東美、荒川浩一、滝沢琢己
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡県
  • Year and Date: 2012-12-12
• [Presentation] 神経幹細胞分化での遺伝子座の核内配置の変動解析 (2012)
  • Author(s): 伊藤謙治、魚崎祐一、野口東美、荒川浩一、滝沢琢己
  • Organizer: 第59回北関東医学会総会
  • Place of Presentation: 群馬県
  • Year and Date: 2012-09-27
• [Presentation] 成熟ニューロンにおける複製依存性ヒストンのダイナミズム解析 (2012)
  • Author(s): 魚崎祐一、野口東美、伊藤謙治、荒川浩一、滝沢琢己
  • Organizer: 第59回北関東医学会総会
  • Place of Presentation: 群馬県
  • Year and Date: 2012-09-27
• [Presentation] ニューロン成熟過程における核内構造変換と遺伝子発現機構の解明 (2012)
  • Author(s): 野口東美、伊藤謙治、魚崎祐一、荒川浩一、滝沢琢己
  • Organizer: 第59回北関東医学会総会
  • Place of Presentation: 群馬県
  • Year and Date: 2012-09-27
• [Presentation] 神経細胞における細胞核構造解析 (2012)
  • Author(s): 滝沢琢己
  • Organizer: 第39回日本毒性学会学術年会
  • Place of Presentation: 宮城県
  • Year and Date: 2012-07-17
• [Presentation] 神経細胞における細胞核構造解析 (2012)
  • Author(s): 滝沢琢己
  • Organizer: 第３９回日本毒性学会学術年会
  • Place of Presentation: 仙台市
  • Invited
• [Presentation] 神経幹細胞における遺伝子座核内配置と転写活性の関連 (2012)
  • Author(s): 伊藤謙治、魚崎祐一、野口東美、荒川 浩一、滝沢琢己
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Invited
• [Presentation] ニューロン成熟過程における核内構造変換と遺伝子発現機構の解明 (2012)
  • Author(s): 野口東美、伊藤謙治、魚崎祐一、荒川浩一、滝沢琢己
  • Organizer: 第59回北関東医学会総会
  • Place of Presentation: 前橋市
  • Invited
• [Presentation] 成熟ニューロンにおける複製依存性ヒストンのダイナミズム解析 (2012)
  • Author(s): 魚崎祐一、野口東美、伊藤謙治、荒川浩一、滝沢琢己
  • Organizer: 第59回北関東医学会総会
  • Place of Presentation: 前橋市
  • Invited
• [Presentation] 神経幹細胞分化勝て員での遺伝子座の核内配置の変動解析 (2012)
  • Author(s): 伊藤謙治、魚崎祐一、野口東美、荒川浩一、滝沢琢己
  • Organizer: 第59回北関東医学会総会
  • Place of Presentation: 前橋市
  • Invited
• [Presentation] 神経幹細胞の性質変換に伴う遺伝子座の核内配置の変動 (2011)
  • Author(s): 伊藤謙治、中島欽一、荒川浩一、滝沢琢己
  • Organizer: 第58回北関東医学会総会
  • Place of Presentation: 群馬県
  • Year and Date: 2011-09-29
• [Presentation] 神経幹細胞性質変換に伴う遺伝子座の核内配置変動解析 (2011)
  • Author(s): 伊藤謙治、中島欽一、荒川浩一、滝沢琢己
  • Organizer: 第58回北関東医学会総会
  • Place of Presentation: 前橋
  • Year and Date: 2011-09-29
• [Presentation] 神経幹細胞性質変換に伴う遺伝子座の核内配置変動解析 (2011)
  • Author(s): 伊藤謙治、中島欽一、荒川浩一、滝沢琢己
  • Organizer: KEY Forum in Developmental Biology and Regenerative Medicine
  • Place of Presentation: 熊本
  • Year and Date: 2011-09-09
• [Presentation] Epigenetics関連技術である細胞核構造解析とその毒性学的研究への適用の可能性 (2011)
  • Author(s): 滝沢琢己
  • Organizer: 第38回日本トキシコロジー学会学術年会
  • Place of Presentation: 神奈川県
  • Year and Date: 2011-07-11
• [Presentation] 神経幹細胞性質変換に伴う遺伝子座の核内配置の変動解析 (2011)
  • Author(s): 伊藤謙治、中島欽一、荒川浩一、滝沢琢己
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: 熊本県
  • Year and Date: 2011-05-19
• [Presentation] 神経幹細胞性質変換に伴う遺伝子座の核内配置変動解析 (2011)
  • Author(s): 伊藤謙治、中島欽一、荒川浩一、滝沢琢己
  • Organizer: 第5回エピジェネティクス研究会
  • Place of Presentation: 熊本
  • Year and Date: 2011-05-19
• [Presentation] 神経活動依存性遺伝子発現の時空間的制御 (2011)
  • Author(s): 滝沢琢己
  • Organizer: 日本農芸化学会 2011年度大会
  • Place of Presentation: 京都女子大学(予定が中止となり、講演予稿集の発行をもって成立)
  • Year and Date: 2011-03-28
• [Presentation] Identification and Spatio-temporal Regulation of Distinct Classes of Activity-dependent Genes in Post-mitotic Neurons (2011)
  • Author(s): 滝沢琢己
  • Organizer: International Symposium on the Physicochemical Field for Genetic Activities
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2011-01-25
• [Presentation] Identification and Spatio-temporal Regulation of Distinct Classes of Activity-dependent Genes in Post-mitotic Neurons (2011)
  • Author(s): Takumi Takizawa
  • Organizer: International Symposium on the Physicochemical Fieldfor Genetic Activities
  • Place of Presentation: 兵庫県
  • Year and Date: 2011-01-24
• [Presentation] 神経系細胞における遺伝子座核内配置 (2010)
  • Author(s): 滝沢琢己
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Analysis of DNA methylation-independent regulatory mechanisms of astrocyte specific gene expression in embryonic stem cells(ESCs) (2010)
  • Author(s): 裏山悟司、滝沢琢己、神山淳、中島欽一
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Analysis of DNA methylation-independent regulatory mechanisms of astrocyte specific gene expression in embryonic stem cells (ESCs) (2010)
  • Author(s): 裏山悟司、滝沢琢己、神山淳、中島欽一
  • Organizer: BMB2010
  • Place of Presentation: 兵庫県
  • Year and Date: 2010-12-07
• [Presentation] 神経系細胞における遺伝子座核内配置 (2010)
  • Author(s): 滝沢琢己
  • Organizer: BMB2010
  • Place of Presentation: 兵庫県
  • Year and Date: 2010-12-07
• [Presentation] Spatiotemporal regulation of activity dependent genes in post-mitotic neurons (2010)
  • Author(s): Takumi Takizawa, Misato Takagi, Kenji Itoh, Kinichi Nakashima
  • Organizer: 40^<th> Annual Meeting NEUROSCIENCE2010
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-15
• [Presentation] Meningeal cells express astrocyte inducing cytokines in the developing mouse brain. (2010)
  • Author(s): Tsukasa Sanosaka, Masakazu Namihira, Takumi Takizawa, Kinichi Nakashima
  • Organizer: 40^<th> Annual Meeting NEUROSCIENCE2010
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-15
• [Presentation] Analysis of DNA methylation independent reguylatory mechanisums of astrocytespecific gene expression in embryonic stem cells. (2010)
  • Author(s): Satoshi Urayama, Takumi Takizawa, Yukina Hori, Jun Kohyama, Kinichi Nakashima
  • Organizer: 40^<th> Annual Meeting NEUROSCIENCE2010
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-15
• [Presentation] Analysis of DNA methylation independent regulatory mechanisms of astrocyte specific gene expression in embryonic stem cells (2010)
  • Author(s): Urayama S., Takizawa T., Hori Y., Kohyama J., Nakashima K.
  • Organizer: 40th Annual Meeting NEUROSCIENCE2010
  • Place of Presentation: San Diego
  • Year and Date: 2010-11-13
• [Presentation] Spatiotemporal regulation of activity dependent genes in post-mitotic neurons (2010)
  • Author(s): Takiazawa, T., Takagi M., Itoh K., Nakashima K.
  • Organizer: 40^<th> Annual Meeting NEUROSCIENCE2010
  • Place of Presentation: San Diego
  • Year and Date: 2010-11-13
• [Presentation] Meningeal Cells Induce Astrocyte Differentiation of Neural Stem Cells. (2010)
  • Author(s): 佐野坂司、波平昌一、滝沢琢己、中島欽一
  • Organizer: The 29^<th> NAITO CONFERENCE ON GLIA WORLD
  • Place of Presentation: 湘南国際村センター
  • Year and Date: 2010-10-06
• [Presentation] Meningeal Cells Induce Astrocyte Differentiation of Neural Stem Cells (2010)
  • Author(s): 佐野坂司、波平昌一、 滝沢琢己 、中島欽一
  • Organizer: The 29^<th> NAITO CONFERENCE ON GLIA WORLD
  • Place of Presentation: 神奈川県
  • Year and Date: 2010-10-05
• [Presentation] アストロサイト分化誘導性サイトカイン発現細胞の同定 (2010)
  • Author(s): 佐野坂司、波平昌一、滝沢琢己、中島欽一
  • Organizer: Neuro 2010
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-03
• [Presentation] 神経活動依存性遺伝子発現の時空間的制御 (2010)
  • Author(s): 滝沢琢己、高木美智、笹岡寛敏、伊藤謙治、中島欽一
  • Organizer: Neuro 2010
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-03
• [Presentation] 神経活動依存性遺伝子発現の時空間制御 (2010)
  • Author(s): 滝沢琢己、高木美智、笹岡寛敏、伊藤謙治、中島欽一
  • Organizer: Neuro2010
  • Place of Presentation: 兵庫県
  • Year and Date: 2010-09-02
• [Presentation] アストロサイト分化誘導性サイトカイン発現細胞の同定 (2010)
  • Author(s): 佐野坂司、波平昌一、滝沢琢己、中島欽一
  • Organizer: Neuro2010
  • Place of Presentation: 兵庫県
  • Year and Date: 2010-09-02
• [Presentation] 胚性幹細胞におけるGFAP遺伝子の発現制御機構の解析 (2010)
  • Author(s): 裏山悟司、滝沢琢己、堀由貴奈、神山淳、中島欽一
  • Organizer: 日本分子生物学会第10回春季シンポジウム
  • Place of Presentation: 宮城県
  • Year and Date: 2010-06-08
• [Presentation] 胚性幹細胞におけるGFAP遺伝子の発現制御機構の解析 (2010)
  • Author(s): 裏山悟司、滝沢琢己、堀由貴奈、神山淳、中島欽一
  • Organizer: 日本分子生物学会 第10回春季シンポジウム
  • Place of Presentation: ホテル松島大観荘
  • Year and Date: 2010-06-08
• [Presentation] Spatiotemporal regulation of activity-dependent genes in post-mitotic neurons (2010)
  • Author(s): Misato Tkagi, Hirotoshi Sasaoka, Kenji Itoh, Hiroshi Kimura, Kinichi Nakashima, Takumi Takizawa
  • Organizer: The 75^<th> Cold Spring Harbor Symposium : Nuclear Organization & Function
  • Place of Presentation: Cold Spring Harbor Labor atory, NY, USA
  • Year and Date: 2010-06-05
• [Presentation] Spatiotemporal regulation of activity dependent gene expression in post-mitotic neurons (2010)
  • Author(s): Takagi M., Sasaoka H., Itoh K., Kimura H., Nakashima K., Takizawa T.
  • Organizer: The 75^<th> Cold Spring Harbor Symposium
  • Place of Presentation: New York
  • Year and Date: 2010-06-02
• [Presentation] 神経活動依存性転写の時空間的制御 (2010)
  • Author(s): 滝沢琢己、高木美智、伊藤謙治、中島欽一
  • Organizer: 第9回 核ダイナミクス研究会
  • Place of Presentation: ラフォーレ修善寺
  • Year and Date: 2010-05-28
• [Book] 神経系細胞とクロマチン高次構造 (2012)
  • Author(s): 伊藤謙治、魚崎祐一、滝沢琢己
  • Publisher: 細胞工学
• [Book] 細胞工学31（8） (2012)
  • Author(s): 伊藤謙治、魚崎祐一、滝沢琢己
  • Total Pages: 6
  • Publisher: 秀潤社
• [Book] ゲノムDNAの核内配置と遺伝子発現制御 (2010)
  • Author(s): 滝沢琢己,高木美智,笹岡寛敏
  • Publisher: 生化学