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Genetic Engineering of Algae for Enhanced Lipid Production

Research Project

Project/Area Number 22681010
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Environmental technology/Environmental materials
Research InstitutionTokyo Institute of Technology (2011-2012)
Chuo University (2010)

Principal Investigator

IMAMURA Sousuke  東京工業大学, 資源化学研究所, 准教授 (70548122)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords環境負荷低減技術 / バイオマスエネルギー / 微細藻類 / バイオマス / 脂質 / 形質転換 / 緑藻 / パーティクルガン
Research Abstract

To improve the lipid productivity in microalga with genetic engineering, a genetic transformation system was established for Pseudochoricystis ellipsoidea. Analyses of transcriptome and proteome revealed candidate genes for the lipid production. Based on the technique and information, I tried to obtain transformants in which each candidate gene is constitutively expressed in the cells.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (51 results)

All 2013 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 6 results) Presentation (39 results) (of which Invited: 2 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Nitrogen assimilatory pathway and the regulation in a unicellular red alga Cyanidioschyzon merolae2012

    • Author(s)
      Kan Tanaka and Sousuke Imamura
    • Journal Title

      The Japanese Society of Photosynthesis Research

      Volume: 22(3) Pages: 167-173

    • NAID

      40019553711

    • URL

      http://photosyn.c.u-tokyo.ac.jp

    • Related Report
      2012 Final Research Report
  • [Journal Article] Genetic transformation of <I>Pseudochoricystis ellipsoidea</I>, an aliphatic hydrocarbon-producing green alga2012

    • Author(s)
      Sousuke Imamura, Daisuke Hagiwara, Fumi Suzuki, Norihide Kurano, and Shigeaki Harayama
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 58 Issue: 1 Pages: 1-10

    • DOI

      10.2323/jgam.58.1

    • NAID

      130004802212

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] External light conditions and internal cell cycle phases entrain accumulation of chloroplast and mitochondrial transcripts in the red alga Cyanidioschyzon merolae2012

    • Author(s)
      Yu Kanesaki, Sousuke Imamura, Ayumi Minoda, Kan Tanaka
    • Journal Title

      DNA Research

      Volume: 19(3) Issue: 3 Pages: 289-303

    • DOI

      10.1093/dnares/dss013

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nitrogen assimilatory pathway and the regulation in a unicellular red alga Cyanidioschyzon merolae2012

    • Author(s)
      Kan Tanaka and Sousuke Imamura
    • Journal Title

      The Japanese Society of Photosynthesis Research

      Volume: 22 Pages: 167-173

    • NAID

      40019553711

    • Related Report
      2012 Annual Research Report
  • [Journal Article] A tetrapyrrole-regulated ubiquitin ligase controls algal nuclear DNA replication2011

    • Author(s)
      Yuki Kobayashi, Sousuke Imamura, Mitsumasa Hanaoka, and Kan Tanaka
    • Journal Title

      Nature Cell Biol.

      Volume: 13(4) Issue: 4 Pages: 483-487

    • DOI

      10.1038/ncb2203

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nitrate assimilatory genes and their transcriptional regulation in a unicellular red alga Cyanidioschyzon merolae : genetic evidence for nitrite reduction by a sulfite reductase-like enzyme2010

    • Author(s)
      Imamura, S., Terashita, M., Ohnuma, M., Maruyama, S., Minoda, A., Weber, A.P., Inouye, T., Sekine, Y., Fujita, Y., Omata, T., Tanaka, K.
    • Journal Title

      Plant and Cell Physiology

      Volume: 51 Issue: 5 Pages: 707-717

    • DOI

      10.1093/pcp/pcq043

    • NAID

      10027348990

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] シゾンの分子遺伝学的解析法の開発2010

    • Author(s)
      大沼 みお、吉田大和、今村壮輔、田中寛、黒岩常祥
    • Journal Title

      生物工学会誌

      Volume: 88(9) Pages: 473-476

    • NAID

      110007730758

    • URL

      https://www.sbj.or.jp/wp-content/uploads/file/sbj/8809_tokushu_3.pdf

    • Related Report
      2012 Final Research Report
  • [Journal Article] Nitrate as similatory genes and their transcriptional regulation in a unicellular red alga Cyanidioschyzon merolae : Genetic evidence for nitrite reduction by a sulfite reductase-like enzyme2010

    • Author(s)
      Sousuke Imamura
    • Journal Title

      Plant Cell Physiology

      Volume: 51 Pages: 707-717

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A tetrapyrrole-regulated ubiquitin ligase controls algal nuclear DNA replication

    • Author(s)
      Yuki Kobayashi
    • Journal Title

      Nature Cell Biology

      Volume: (掲載決定(印刷中))

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] シゾン核コードシグマ因子 SIG2による葉緑体フィコビリソーム遺伝子群の転写活性化2013

    • Author(s)
      藤井岳、今村壮輔、華岡光正、田中寛
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2012 Final Research Report
  • [Presentation] 単細胞紅藻 Cyanidioschyzon merolaeにおける分裂期特異的なヒストン H3K9のアセチル化の解析2013

    • Author(s)
      曾根俊之、今村壮輔、華岡光正、黒岩常祥、田中寛
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2012 Final Research Report
  • [Presentation] 単細胞性緑藻 Pseudochoricystis ellipsoideaを用いた、バイオ燃料生産のための有用形質を持つ突然変異体のゲノム解析2013

    • Author(s)
      井出曜子、早川准平、坂本美佳、今村壮輔、蔵野憲秀、原山重明
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      滋賀
    • Related Report
      2012 Final Research Report
  • [Presentation] 微細藻類によるバイオ燃料生産 Pseudococcomyxa ellipsoideaにおける油脂合成関連因子の同定に向けて2013

    • Author(s)
      並木友亮、今村壮輔、藏野憲秀、原山重明
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      滋賀
    • Related Report
      2012 Final Research Report
  • [Presentation] オイル生産藻類 Pseudochoricystis ellipsoideaにおける安定した導入遺伝子発現系の開発2013

    • Author(s)
      阿部淳、高木さつき、吉満勇也、福原いずみ、藏野憲秀、今村壮輔、原山重明
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      滋賀
    • Related Report
      2012 Final Research Report
  • [Presentation] 微細藻類 Pseudochoricystis ellipsoidea の遺伝子ノックアウト技術開発2013

    • Author(s)
      吉満勇也、今村壮輔、福原いずみ、藏野憲秀、福田裕章、原山重明
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      滋賀
    • Related Report
      2012 Final Research Report
  • [Presentation] 単細胞性緑藻 Pseudochoricystis ellipsoidea を用いたバイオ燃料生産のための有用形質を持つ突然変異体のゲノム解析2013

    • Author(s)
      井出 曜子
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大学(滋賀県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 微細藻類によるバイオ燃料生産― PseudoCoccomyxa ellipsoidea における油脂合成関連因子の同定に向けて―2013

    • Author(s)
      並木 友亮
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大学(滋賀県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] オイル生産藻類 Pseudochoricystis ellipsoidea における安定した導入遺伝子発現系の開発2013

    • Author(s)
      阿部 淳
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大学(滋賀県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 微細藻類 Pseudochoricystis ellipsoidea の遺伝子ノックアウト技術開発2013

    • Author(s)
      吉満 勇也
    • Organizer
      第7回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大学(滋賀県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Nitrogen assimilatory pathway and the regulation in a eukaryotic red alga Cyanidioschyzon merolae2012

    • Author(s)
      Kan Tanaka, Tsubasa Hosoya, Masaru Terashita, Yu Kanesaki and Sousuke Imamura
    • Organizer
      Physiology and Biotechnology of Microalgae, Russian Academy of Science
    • Place of Presentation
      Moscow, Russia
    • Year and Date
      2012-10-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 紅藻シゾン Cyanidioschyzon merolaeにおける代謝制御研究2012

    • Author(s)
      田中寛、今村壮輔
    • Organizer
      光合成学会公開シンポジウム「光合成と藻類バイオテクノロジー」
    • Place of Presentation
      横浜
    • Year and Date
      2012-06-01
    • Related Report
      2012 Final Research Report
  • [Presentation] 単細胞紅藻シゾンにおける分裂期特異的なヒストンH3K9のアセチル化と転写活性化における役割2012

    • Author(s)
      曾根俊之
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物で初めて同定された窒素同化を制御する転写因子2012

    • Author(s)
      今村壮輔
    • Organizer
      第6回日本ゲノム微生物学会年会
    • Place of Presentation
      立教大学
    • Year and Date
      2012-03-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 油脂生産性微細藻類によるバイオ燃料生産.Pseudochoricystis ellipsoideaにおける油脂合成関連遺伝子の同定に向けて2012

    • Author(s)
      並木友亮、今村壮輔、藏野憲秀、原山重明
    • Organizer
      第6回日本ゲノム微生物学会若手の会
    • Place of Presentation
      静岡
    • Related Report
      2012 Final Research Report
  • [Presentation] 微細藻類 Pseudochoricystis ellipsoideaの遺伝子改変2012

    • Author(s)
      吉満勇也、今村壮輔、福原いずみ、藏野憲秀、福田裕章、原山重明
    • Organizer
      第6回日本ゲノム微生物学会若手の会
    • Place of Presentation
      静岡
    • Related Report
      2012 Final Research Report
  • [Presentation] R2R3-type MYB transcription factor, CmMYB1, is a central nitrogen assimilation regulator in Cyanidioschyzon merolae2012

    • Author(s)
      Sousuke Imamura, Yu Kanesaki, Mio Ohnuma, Takayuki Inouye,Yasuhiko Sekine, Takayuki Fujiwara, Tsuneyoshi Kuroiwa, Kan Tanaka
    • Organizer
      The 12th Asian Conference on Transcription
    • Place of Presentation
      Cheju Island, Korea
    • Related Report
      2012 Final Research Report
  • [Presentation] 単細胞紅藻シゾンにおける分裂期特異的なヒストン H3K9のアセチル化と転写活性化における役割2012

    • Author(s)
      曾根俊之、廣岡俊亮、今村壮輔、黒岩常祥、華岡光正、田中寛
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] 植物で初めて同定された窒素代謝を制御する転写因子2012

    • Author(s)
      今村壮輔、兼崎友、大沼みお、井上貴之、関根靖彦、藤原崇之、黒岩常祥、田中寛
    • Organizer
      第6回日本ゲノム微生物学会年会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] R2R3-type MYB transcription factor, CmMYB1, is a central nitrogen assimilation regulator in Cyanidioschyzon merolae2012

    • Author(s)
      Sousuke Imamura
    • Organizer
      The 12th Asian Conference on Transcription
    • Place of Presentation
      Seogwipo KAL hotel(Korea)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Genetic analysis of "Pseudochoricystis ellipsoidea", an aliphatic hydrocarbon-producing green alga2011

    • Author(s)
      Sousuke Imamura, Hagiwara Daisuke, Norihide Kurano, Shigeaki Harayama
    • Organizer
      International Symposium on Algal Biofuels
    • Place of Presentation
      Tokyo
    • Year and Date
      2011-11-17
    • Related Report
      2012 Final Research Report
  • [Presentation] Genetic analysis of "Pseudochoricystis ellipsoidea", an aliphatic hydrocarbon-producing green alga2011

    • Author(s)
      Sousuke Imamura
    • Organizer
      International Symposium on Algal Biofuels
    • Place of Presentation
      文京シビックホール
    • Year and Date
      2011-11-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 軽油を生産する単細胞緑藻"Pseudochoricystis ellipsoidea"-分子生物学的手法を用いた軽油生産性向上への取り組み-2011

    • Author(s)
      今村壮輔
    • Organizer
      第5回日本ゲノム微生物学会若手の会
    • Place of Presentation
      労金研修所富士センター
    • Year and Date
      2011-09-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeにおける窒素飢餓応答機構の解析2011

    • Author(s)
      細谷翼
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東北大学
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物で初めて同定された窒素同化を制御する転写因子2011

    • Author(s)
      今村壮輔
    • Organizer
      日本ゲノム微生物学会
    • Place of Presentation
      東北学院大学
    • Year and Date
      2011-03-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 軽油を生産する単細胞緑藻 "Pseudochoricystis ellipsoidea".分子生物学的手法を用いた軽油生産性向上への取り組み2011

    • Author(s)
      今村壮輔、藏野憲秀、原山重明
    • Organizer
      第5回日本ゲノム微生物学会若手の会
    • Place of Presentation
      静岡
    • Related Report
      2012 Final Research Report
  • [Presentation] 植物で初めて同定された窒素代謝を制御する転写因子2011

    • Author(s)
      今村壮輔、兼崎友、大沼みお、井上貴之、関根靖彦、藤原崇之、黒岩常祥、田中寛
    • Organizer
      第5回日本ゲノム微生物学会年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] 炭化水素生産性緑藻 "Pseudochoricystis ellipsoidea"のゲノム構造2011

    • Author(s)
      原山重明、今村壮輔、岩舘満雄、近藤伸二、小沢理津子、足立直樹、Todd Taylor、鈴木穣、菊池淳、蔵野憲秀
    • Organizer
      第5回日本ゲノム微生物学会年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] 単細胞紅藻 Cyanidioschyzon merolaeにおける窒素飢餓応答機構の解析2011

    • Author(s)
      今村壮輔、細矢翼、華岡光正、田中寛
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] 植物で初めて同定された窒素代謝を制御する転写因子-バイオ燃料生産への応用に向けて-2010

    • Author(s)
      今村壮輔
    • Organizer
      日本光合成学会若手の会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-10-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 単細胞紅藻シゾンで初めて明らかにされたリボソーム合成系の進化過程2010

    • Author(s)
      今村壮輔
    • Organizer
      日本ゲノム微生物学会若手の会
    • Place of Presentation
      神戸セミナーハウス
    • Year and Date
      2010-10-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] R2R3-type MYB transcription factor, CmMYB1, is a central nitrogen assimilation regulator in Cyanidioschyzon merolae2010

    • Author(s)
      Sousuke Imamura , Yu Kanesaki, Mio Ohnuma, Takayuki Inouye,Yasuhiko Sekine, Takayuki Fujiwara, Tsuneyoshi Kuroiwa, Kan Tanaka
    • Organizer
      Microalgal Biotechnology-"Food・Environment・Energy"
    • Place of Presentation
      Tokyo
    • Year and Date
      2010-05-31
    • Related Report
      2012 Final Research Report
  • [Presentation] 単細胞紅藻シゾンで初めて明らかにされたリボソーム合成系の進化過程2010

    • Author(s)
      今村壮輔
    • Organizer
      第4回日本ゲノム微生物学会若手の会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] シゾン核コードシグマ因子SIG2による葉緑体フィコビリソーム遺伝子群の転写活性化

    • Author(s)
      藤井 岳
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeにおける分裂期特異的なヒストンH3K9のアセチル化の解析

    • Author(s)
      曾根 俊之
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Nitrogen assimilatory pathway and the regulation in a eukaryotic red alga Cyanidioschyzon merolae

    • Author(s)
      Kan Tanaka
    • Organizer
      Physiology and Biotechnology of Microalgae, Russian Academy of Science
    • Place of Presentation
      Moscow(Russia)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 油脂生産性微細藻類によるバイオ燃料生産―Pseudochoricystis ellipsoideaにおける油脂合成関連遺伝子の同定に向けて―

    • Author(s)
      並木 友亮
    • Organizer
      第6回日本ゲノム微生物学会若手の会
    • Place of Presentation
      ろうきん研修所富士センター(静岡県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 微細藻類 Pseudochoricystis ellipsoidea の遺伝子改変

    • Author(s)
      吉満 勇也
    • Organizer
      第6回日本ゲノム微生物学会若手の会
    • Place of Presentation
      ろうきん研修所富士センター(静岡県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 紅藻シゾンCyanidioschyzon merolaeにおける代謝制御研究

    • Author(s)
      田中 寛
    • Organizer
      光合成学会公開シンポジウム「光合成と藻類バイオテクノロジー」
    • Place of Presentation
      東京工業大学(神奈川県)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] 軽油生産能を有する単細胞緑藻の生産性向上 微細藻類によるエネルギー生産と事業展望2012

    • Author(s)
      藏野 憲秀、萩原 大祐、今村 壮輔、原山重明
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Final Research Report
  • [Book] 微細藻類によるエネルギー生産と事業展望2012

    • Author(s)
      藏野 憲秀
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.res.titech.ac.jp/%7Ebiores/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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