Functionalized conducting polymer biointerfaces for cell engineering: specific adhesion of proteins and cells, cell patterning, and dynamic binding/releasing by electrical stimuli.
| Project/Area Number |
22681016
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
YU Hsiao-hua 独立行政法人理化学研究所, Yu 独立主幹研究ユニット, ユニットリーダー(独立主幹研究員) (70529908)
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| Research Collaborator |
TSENG Hsian-rong University of California, Los Angeles, Department of Molecular and Medical Pharmacology, Professor,
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2010: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
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| Keywords | 有機化学 / 導電性高分子 / 有機導体 / バイオテクノロジー / バイオインターフェイス / ナノバイオ / ナノ材料 |
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| Research Abstract |
During the 3-year of this original project, we have successfully achieved all the original goals and build a new concept to utilize molecularly designed conducting polymer to control cellular behavior on the interface between cells and materials. The interface is also dynamic therefore we can control the interface by both chemical and electrical stimulation. The major achievements include: (1) Successfully build a monomer library consists of more than 20 new thiophene based monomers. (2) Control the cellular interaction with the materials with introduction of the monomers that prohibit proteins and cells binding to the materials. (3) Switch the surface properties from layered polythiophenes assemblies. (4) Cell patterning with top-down fabrication of bottom-up electropolymerization assemblies. (5) Dynamic polythiophene-cell interface with electrical stimulation. We further studied the effect of chemical and electrical stimulation on the cell engineering with these materials. The major results include: (1) Conducting polymer nano-networks for cell engineering. Thiophene monomer with a variety of functional groups would form EDOT nano-networks with the nanofiber dimensions between 20 to 40 nm by controlling the electropolymerization kinetics. (2) Enhanced cell-capturing on the nanostructured conducting polymer with capture agents. More efficient capturing of cancer cells were demonstrated on antibody conjugated polythiophene nanodots.
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Report
(4 results)
Research Products
(102 results)
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[Journal Article]2013
Author(s)
Zhao, H.; Zhu, B.; Luo, S.-C.; Lin, H.-A.; Nakao, A.; Yamashita, Y.; Yu, Hsiao-hua
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Journal Title
ACS Appl. Mater. Interfaces
Volume: (referred)
Issue: 11
Pages: 4536-4543
DOI
Related Report
Peer Reviewed
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[Journal Article]2013
Author(s)
Lin, H.-A.; Luo, S.-C.; Zhu, B.; Yamashita, Y.; Yu, Hsiao-hua
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Journal Title
Adv. Funct. Mater.
Volume: 25(referred)
Issue: 25
Pages: 3212-3219
DOI
Related Report
Peer Reviewed
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[Journal Article]2013
Author(s)
Hou, S.; Zhao, H.; Zhao, L.; Shen, Q.; Wei, K. S.; Suh D. Y.; Nakao, A.; Xiong B.; Luo, S.-C.; Tseng, H.-R.; Yu, Hsiao-hua
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Journal Title
Adv. Mater.
Volume: 25
Pages: 1547-1551
Related Report
Peer Reviewed
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[Journal Article]2012
Author(s)
Zhao, H.; Liu, C.-H.; Luo, S.-C.; Zhu, B.; Wang, T.-H.; Hsu, H.-F.; Yu, Hsiao-hua
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Journal Title
Macromolecules
Volume: 45
Pages: 7783-7790
Related Report
Peer Reviewed
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[Journal Article]2012
Author(s)
Luo, S.-C.; Zhu, B.; Kantchev, E. A. B.; Siang, Y. W.; Yu, Hsiao-hua
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Journal Title
Chem. Commun.
Volume: 48
Pages: 6942-6944
Related Report
Peer Reviewed
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[Journal Article]2012
Author(s)
Lacher, S.; Obata, N.; Luo, S.-C.; Matsuo, Y.; Zhu, B.; Yu, Hsiao-hua; Nakamura, E
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Journal Title
ACS Appl. Mater. Interfaces
Volume: 4
Pages: 3396-3404
Related Report
Peer Reviewed
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[Journal Article]2012
Author(s)
Luo, S.-C.; Sekine, J.; Zhu, B.; Zhao, H.; Nakao, A.; Yu, Hsiao-hua
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Journal Title
ACS Nano
Volume: 6
Pages: 3018-3026
Related Report
Peer Reviewed
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[Journal Article]2012
Author(s)
Zhao, H.; Zhu, B.; Sekine, J.; Luo, S.-C.; Yu, Hsiao-hua
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Journal Title
ACS Appl. Mater. Interfaces
Volume: 4
Pages: 680-686
Related Report
Peer Reviewed
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[Journal Article]2012
Author(s)
Guo, Z.; Hu, L.; Yu, Hsiao-hua; Can, X.; Gu, H.
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Journal Title
RSC Advances
Volume: 2
Pages: 3477-3480
Related Report
Peer Reviewed
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[Journal Article]2011
Author(s)
Sekine, J.; Luo, S.-C.; Wang, S.; Zhu, B.; Tseng, H.R.; Yu, Hsiao-hua
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Journal Title
Adv. Mater
Volume: 23
Pages: 4788-4792
Related Report
Peer Reviewed
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[Journal Article]2011
Author(s)
Luo, S.-C.; Zhu, B.; Nakao, A.; Nakatomi, R.; Yu, Hsiao-hua
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Journal Title
Adv. Eng. Mater. (Advanced Biomaterials)
Volume: 13
Related Report
Peer Reviewed
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[Journal Article]2011
Author(s)
Tansil, N. C.; Kantchev, E. A. B.; Gao, Z.; Yu, Hsiao-hua
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Journal Title
Chem. Commun.
Volume: 47
Pages: 1533-1535
Related Report
Peer Reviewed
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[Presentation] Soft Nanomaterials for E2013
Author(s)
Hsiao-hua Yu
Organizer
Soft-interfaces Mini-symposium 2013 -Physical Chemistry and Characterization of Softinterfaces (SIMS2013)
Place of Presentation
Kyushu University, Fukuoka
Related Report
Invited
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[Presentation] The e2013
Author(s)
Jun Sekine
Organizer
Soft-interfaces Mini-symposium 2013 -Physical Chemistry and Characterization of Softinterfaces (SIMS2013)
Place of Presentation
Kyushu University, Fukuoka
Related Report
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