| Budget Amount *help |
¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2010: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Research Abstract |
I conducted integrated transcriptome analysis of a colon cancer cell line, DLD-1, in response to hypoxic shock using next generation sequencer. I generated 100 million TSS tags and associated a wide variety of epigenomic and transcriptomic information. Namely, this dataset include ChIP Seq data of HIF1, which is a pivotal transcription factor in response to hypoxic shock, representative active and repressive histone markers and RNApolymerase II. This data was further associated with RNA Seq data of nucleus, cytoplasm and polysome fractions. Integrative analysis of the data revealed that chromatin forms open structure in prior to actual hypoxic shock. We also demonstrate that gene expression regulations are exerted at various levels of transcriptome layers, such as epigenomic regulations, transcriptional initiation, elongations and finally degradations. This is the first report shed light on comprehensive views on the transcriptome regulations in cancer cells in hypoxic conditions, thus, should give useful clues for the future analysis how cancers survive in hypoxic environments in vivo.
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