Development of genetic resources preservation for domestic animals using freeze-dried somatic cells
| Project/Area Number |
22681035
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Resource conservation science
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| Research Institution | Kochi University |
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Principal Investigator |
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2010: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | 遺伝子資源保全 / 凍結乾燥 / 牛 / 核移植 / 家畜 / 遺伝資源保存・再生 / 哺乳動物体細胞の保存 / 遺伝資源保存 / ウシ |
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| Research Abstract |
Freeze-dried somatic cells have been proposed as a new tool for production of clone animals, since it can overcome disadvantages of the current cryopreservation method. Here, we investigated the DNA damage on bovine fibroblast cells after lyophilization, and the influence of storage temperature of FD cells on in vitro development of oocytes after NT. The rate of DNA-damaged cells was 22% in FD cells preserved at -30C, and 100% at +20C. The cleavage rates of NT embryos with fresh cells, FD cells stored at -30C, and those stored at +20C were 67%, 50%, and 0%, respectively. The blastocyst formation rates were 37%, 28%, and 0%, respectively. No significant differences were found between FD cells stored at -30C and fresh cells with regard to cleavage and development to the blastocyst stage. These results suggest that bovine blastocysts are successfully produced from FD cell-NT oocytes.
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Report
(5 results)
Research Products
(45 results)
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[Journal Article] Development of single blastomeres derived from two-cell embryos produced in vitro in pigs2011
Author(s)
Dang-Nguyen TQ, Kaneda M, Somfai T, Haraguchi S, Matsukawa K, Akagi S, Kikuchi K, Nakai M, Nguyen BX, Tajima A, Kanai Y, Nagai T
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Journal Title
Theriogenology
Volume: 76
Pages: 88-96
Related Report
Peer Reviewed
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[Journal Article] Pinkert, Microinjection of serum-starved mitochondria derived from somatic cells affects parthenogetic development of bovine and murine oocytes2010
Author(s)
Kumiko Takeda, Mariko Tasai, Satoshi Akagi, Kazutsugu Matsukawa, Seiya Takahashi, Masaki Iwamoto, Kanokwan Srirattana, Akira Onishi, Takahiro Tagami, Keijiro Nirasawa, Hirofumi Hanada, Carl A
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Journal Title
Mitochondrion
Volume: 10
Pages: 137-142
Related Report
Peer Reviewed
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[Journal Article] Design and fabrication of all-in-one unified microfluidic chip for automation of embryonic cell manipulation2010
Author(s)
Yoko Yamanishi, Shinya Sakuma, Tomohiro Iyanagi, Fumihito Arai, Tatsuo Arai, Akiyuki Hasegawa, Tamio Tanikawa, Akihiko Ichikawa, Osamu Sato, Akihiro Nakayama, Hiroshi Aso, Mitsuhiro Goto, Seiya Takahashi, Kazutsugu Matsukawa
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Journal Title
Journal of Robotics and Mechatronics
Volume: 21
Pages: 371-379
Related Report
Peer Reviewed
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[Presentation] Significant improvement of blastocyst yield by production of twin blastosysts derived from two sister blastomeres of 2-cell embryos in pigs2010
Author(s)
Thanh Quang Dang-Nguyen, Masahiro Kaneda, Tamas Somfai, Kazutsugu Matsukawa, Satoshi Akagi, Kazuhiro Kikuchi, Michiko Nakai, Bui Xuan Nguyen, Atsushi Tajima, Yukio Kanai, Takashi Nagai
Organizer
第103回日本繁殖生物学会
Place of Presentation
十和田市
Related Report
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