Intra-cellular environment monitoring to clarify the biominelarization process of calcareous foraminifera

• Project/Area Number: 22684027
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Stratigraphy/Paleontology
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: TOYOFUKU Takashi 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, チームリーダー (30371719)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
• Keywords: バイオミネラリゼーション / 有孔虫 / 石灰化 / カルシフィケーション / 古生物学 / 海洋生物学 / 地球科学 / 原生生物 / 細胞内環境 / 実験古生物学 / 蛍光観察 / 地球生物学

Research Abstract

The process that some minerals actively precipitated by biology are called as biomineralization. Foraminifera is one of the key calcifyer in marine environment. This study try to clarify the biomineraliczation process of foraminifera by cell biology. Intra cellular distributions of calcium pH, magnesium ,mito and golgi complex are visualized by fluorescent dyes. Mineralogy of crystals can be decided even during on-going calcification process.

Research Products

• [Journal Article] Attachment and Detachment of Living Microorganisms Using a Potential-Controlled Electrode (2013)
  • Author(s): Koyama, S., Konishi, M., Ohta, Y., Miwa, T., Hatada, Y., Toyofuku, T., Maruyama, T., Nogi, Y., Kato, C., Tsubouchi, T
  • Journal Title: Marine biotechnology Volume: - Issue: 4 Pages: 461-475
  • DOI: 10.1007/s10126-013-9495-2
  • Peer Reviewed
• [Journal Article] Micro-scale neodymium distribution in sedimentary planktonic foraminiferal tests and associated mineral phases (2013)
  • Author(s): Tachikawa, K., Toyofuku, T., Basile-Doelscha, I., Delhayec, T
  • Journal Title: Geochimica et Cosmochimica Acta Volume: 100 Pages: 11-23
  • DOI: 10.1016/j.gca.2012.10.010
  • Peer Reviewed / Open Access
• [Journal Article] Effects of Hydrogen Sulfide on Bacterial Communities on the Surface of Galatheid Crab, <i>Shinkaia crosnieri</i>, and in a Bacterial Mat Cultured in Rearing Tanks (2013)
  • Author(s): Konishi, M., Watsuji, T., Nakagawa, S., Hatada, Y., Takai, K., Toyofuku T
  • Journal Title: Microbes and Environments Volume: 28 Issue: 1 Pages: 25-32
  • DOI: 10.1264/jsme2.ME12070
  • NAID: 10031138540
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed
• [Journal Article] Variation in stable carbon and oxygen isotopes of individual benthic foraminifera: tracers for quantifying the vital effect (2012)
  • Author(s): Ishimura T, Tsunogai U, Hasegawa S, Nakagawa F, Oi T, Kitazato H, Suga H, Toyofuku T
  • Journal Title: Biogeosciences Discuss Volume: 9 Pages: 6191-6218
  • DOI: 10.5194/bgd-9-6191-2012
  • Peer Reviewed
• [Journal Article] バイオミネラリゼーションの可視化 -細胞生物学的に見た有孔虫の石灰化過程- (2012)
  • Author(s): 豊福高志
  • Journal Title: Japan Geoscience Letters Volume: 8 Pages: 4-4
• [Journal Article] バイオミネラリゼーションの可視化 - 細胞生物学的に見た有孔虫の石灰化過程 (2012)
  • Author(s): 豊福 高志
  • Journal Title: Japan Geoscience Letters Volume: 8, 4 Pages: 6-6
• [Journal Article] Mg/Ca and δ18O in the brackish shallow-water benthic foraminifer Ammonia 'beccarii' (2011)
  • Author(s): Toyofuku, T., M. Suzuki, H. Suga, S. Sakai, A.Suzuki, T. Ishikawa, L. Nooijer, R. Schiebel, H. Kawahata, H. Kitazato
  • Journal Title: Marine Microplaeontology Volume: 78 Issue: 3-4 Pages: 113-120
  • DOI: 10.1016/j.marmicro.2010.11.003
  • Peer Reviewed
• [Journal Article] 有孔虫の飼育実験から探る太古の地球環境 (2011)
  • Author(s): 豊福高志, 野牧秀隆
  • Journal Title: バイオサイエンスとインダストリー Volume: 69, 4 Pages: 284-288
  • NAID: 10029236996
• [Journal Article] 有孔虫の飼育実験から探る太古の地球環境 (2011)
  • Author(s): 豊福高志、野牧秀隆
  • Journal Title: バイオサイエンスとインダストリー Volume: 69(4) Pages: 284-288
  • NAID: 10029236996
• [Journal Article] The role of meiofauna in deep-sea benthic food webs revealed by carbon and nitrogen stable isotope analyses In Earth, Life, and Isotopes (Eds: Ohkouchi N, Tayasu I, and Koba K) (2010)
  • Author(s): Nomaki, H., Ogawa, N. O., Ohkouchi, N., Toyofuku, T., Kitazato, H
  • Journal Title: Kyoto University Press Pages: 119-138
  • Peer Reviewed
• [Journal Article] アンモニア・ベッカリー 過酷な環境にも生息 (2010)
  • Author(s): 豊福高志
  • Journal Title: しずおか自然史, Pages: 86-87
• [Journal Article] 小さなタイムマシンを開く-有孔虫の石灰化過程の可視化- (2010)
  • Author(s): 豊福高志
  • Journal Title: 生物の科学 遺伝 Volume: 64(5) Pages: 78-85
• [Journal Article] 有孔虫～多様な殻を作る単細胞生物と海洋～ (2010)
  • Author(s): 豊福高志
  • Journal Title: 第4回JAMSTEC海洋と地球の学校 多様性を科学する～海洋と地球のツアー～ Pages: 71-82
• [Journal Article] 海洋酸性化と生物圏への影響 (2010)
  • Author(s): 豊福高志
  • Journal Title: 平成21年度海洋研究開発機構研究報告会JAMSTEC2010 地球システムの解明に果たすJAMSTECの役割 Pages: 8-9
• [Journal Article] he role of meiofauna in deep-sea benthic food-webs revealed by stable isotope analyses (2010)
  • Author(s): Nomaki H, Ogawa NO, Ohkouchi N, Toyofuku T, Kitazato H
  • Journal Title: Earth, Life, and Isotopes : A Tribute to Dr.Eitaro Wada (Eds : Naohiko Ohkouchi, Ichiro Tayasu, and Keisuke Koba) Kyoto University Press Pages: 119-138
  • Peer Reviewed
• [Journal Article] Long-term observation of deep-sea benthic activities in Sagami Bay, central Japan (2010)
  • Author(s): Kitazato, H., Fujikura, K, Furushima, Y, Toyofuku, T, Nomaki, H, Iwase, R
  • Journal Title: Proceedings of OceanObs'09 : Sustained Ocean Observations and Information for Society Volume: 2 Pages: 5-5
• [Journal Article] 小さなタイムマシンを開く-有孔虫の石灰化過程の可視化- (2010)
  • Author(s): 豊福高志
  • Journal Title: 遺伝生物の科学 Volume: 64(5) Pages: 78-85
• [Journal Article] 古生物学事典 (2010)
  • Author(s): 日本古生物学会(編)
  • Journal Title: 古生物学事典 Volume: (一部項目執筆)
• [Journal Article] アンモニア・ベッカリー過酷な環境にも生息 (2010)
  • Author(s): 豊福高志
  • Journal Title: しずおか自然史 Pages: 86-87
• [Presentation] Tohoku Tsunami on shallow-water marine biodiversity: environmental biomonitoring with live benthic foraminifera (2013)
  • Author(s): Duros P
  • Organizer: The Paleontological Society of Japan
  • Place of Presentation: Yokohama, Japan
• [Presentation] Assessing the environmental impact of Tōhoku tsunami off Hachinohe (NE Japan): a multidisciplinary approach (2013)
  • Author(s): Fontanier C
  • Organizer: The Paleontological Society of Japan
  • Place of Presentation: Yokohama, Japan
• [Presentation] High-CO2実験環境下の細胞内pH観察 (2012)
  • Author(s): 豊福高志,Lennart J de Nooijer,野牧秀隆,浅井健志,土屋正史,倉沢篤史,三輪哲也,北里洋
  • Organizer: 古海洋シンポジウム2012
  • Place of Presentation: 東京大学大気海洋研究所
  • Year and Date: 2012-01-06
• [Presentation] A. Tombi Cruise: an Overview (2012)
  • Author(s): Toyofuku, T., Ohkawara W. N., Kurasawa
  • Organizer: TOMBI Workshop
  • Place of Presentation: Bordeaux, France
• [Presentation] TOMBI Cruise core description (2012)
  • Author(s): Fujii, M., Sakaguchi, A., Murayama, M., Toyofuku, T., Oguri, K., Kawamura,K
  • Organizer: TOMBI Workshop
  • Place of Presentation: Bordeaux, France
• [Presentation] Calcification, Field Workshop on Living Foraminifera in Japan (2012)
  • Author(s): Toyofuku, T
  • Organizer: Sesoko
  • Place of Presentation: Okinawa/Yokosuka, Kanagawa, Japan
• [Presentation] Foraminiferal intracellular environmental observation - perspective for biomineralization, geochemical proxies for past time ocean and ocean acidification (2012)
  • Author(s): Toyofuku, T., de Nooijer, L., Nomaki, H., Tsuchiya, M., Oguri, K., Kitazato, H
  • Organizer: 2012 ASLO Aquatic Sciences Meeting
  • Place of Presentation: Otsu, Shiga, Japan
• [Presentation] Reichart, G.J. Species-specific impacts of temperature and seawater Mg/Ca on foraminiferal Mg/Ca (2012)
  • Author(s): van Dijk, I.E.Y., de Nooijer, L.J., Toyofuku, T
  • Organizer: EGU General Assembly 2012
  • Place of Presentation: Vienna, Austria
• [Presentation] Foraminiferal cellular Calcium and pH distribution by laboratory observation (2012)
  • Author(s): Toyofuku, T., Nomaki, H., Tsuchiya, M., Kitazato, H
  • Organizer: EGU General Assembly 2012
  • Place of Presentation: Vienna, Austria
• [Presentation] Oxygen concentration profiles and the consumption rates at the sediment-water interface off Hachinohe, Northeastern Japan (2012)
  • Author(s): Oguri K
  • Organizer: EGU General Assembly 2012
  • Place of Presentation: Vienna, Austria
• [Presentation] pecies-specific impacts of temperature and seawater Mg/Ca on foraminiferal Mg/Ca (2012)
  • Author(s): van Dijk I.E.Y.
  • Organizer: EGU General Assembly 2012
  • Place of Presentation: Vienna, Austria
• [Presentation] Calcification (2012)
  • Author(s): Toyofuku T
  • Organizer: Field Workshop on Living Foraminifera in Japan
  • Place of Presentation: Sesoko, Okinawa/Yokosuka, Kanagawa, Japan
• [Presentation] Tombi Cruise: an Overview (2012)
  • Author(s): Toyofuku T
  • Organizer: TOMBI Workshop
  • Place of Presentation: Bordeaux, France
• [Presentation] Ultra-deep sequencing of ultra-deep sediment (2012)
  • Author(s): Lecroq B
  • Organizer: 13th Deep-Sea Biology Symposium
  • Place of Presentation: Wellington, New Zealand
• [Presentation] Culture experiment with living foraminifera under laboratory high-CO2 environmental condition (2011)
  • Author(s): Toyofuku, T, Nooijer, LJ, Nomaki, H, Tsuchiya, M, Kitazato H
  • Organizer: EGU Topical Conference Series, 7th Alexander von Humbolt International Conferene Ocean acidification
  • Place of Presentation: マレーシア・ペナン
  • Year and Date: 2011-06-21
• [Presentation] Foraminiferal cellular pH control under low pH environment in the laboratory (2011)
  • Author(s): Toyofuku, T., Nooijer, L. J., Nomaki, H., Tsuchiya, M., Kitazato H
  • Organizer: JpGU International session
  • Place of Presentation: Makuhari, Chiba
  • Year and Date: 2011-05-25
• [Presentation] Foraminiferal cellular pH control under low pH environment in the laboratory (2011)
  • Author(s): Toyofuku, T, Nooijer, LJ, Nomaki, H, Tsuchiya, M, Kitazato H
  • Organizer: 日本地球惑星科学連合大会2011
  • Place of Presentation: 千葉市・幕張メッセ
  • Year and Date: 2011-05-25
• [Presentation] pH環境を制御した有孔虫飼育実験 (2011)
  • Author(s): 豊福高志
  • Organizer: 日本地球惑星科学連合大会2011
  • Place of Presentation: 千葉市・幕張メッセ
  • Year and Date: 2011-05-25
• [Presentation] Foraminiferal cellular pH control under high-CO_2 condition in the laboratory (2011)
  • Author(s): Toyofuku T, Nomaki H, Tsuchiya M, Kitazato H
  • Organizer: European Geosciences Union, General Assembly 2011
  • Place of Presentation: ウィーン・オーストリア
  • Year and Date: 2011-04-07
• [Presentation] Linear invese modelと現場実験、観測値を統合した相模湾深海底の炭素循環モデル (2011)
  • Author(s): 野牧秀隆・豊福高志・北里洋・Dick van Oevelen
  • Organizer: 2010年度古海洋シンポジウム
  • Place of Presentation: 東京大学大気海洋研究所、千葉県柏市
  • Year and Date: 2011-01-06
• [Presentation] Culture experiment with living foraminifera under laboratory high-CO2 environmental condition. EGU Topical Conference Series (2011)
  • Author(s): Toyofuku, T., de Nooijer, L. J., Nomaki, H., Tsuchiya, M., Kitazato, H
  • Organizer: 7th Alexander von Humbolt International Conferene Ocean acidification: consequences for marine ecosystems and society
  • Place of Presentation: Park Royal Penang Resort, Penang, Malaysia
• [Presentation] Foraminiferal cellular pH control under laboratory high-CO2 environmental condition (2011)
  • Author(s): Toyofuku, T., de Nooijer, L. J., Nomaki, H., Tsuchiya, M., Kitazato, H
  • Organizer: EGU General Assembly 2011
  • Place of Presentation: Vienna, Austria
• [Presentation] 底生有孔虫Ammonia beccariiのMg/Ca、δ18Oの水温、塩分指標 (2011)
  • Author(s): 豊福高志, 菅 寿美, 坂井三郎, 鈴木淳, 川幡穂高, 北里 洋
  • Organizer: 2010年度古海洋シンポジウム
  • Place of Presentation: 東京大学大気海洋研究所
• [Presentation] Shape design of BaCO3 by Mg2+ effect (2010)
  • Author(s): 林 明子, 豊福高志, 中村利廣, 渡辺友亮
  • Organizer: 第20回日本MRS学術シンポジウム
  • Place of Presentation: 横浜情報文化センター
  • Year and Date: 2010-12-20
• [Presentation] Multiple endobionts of both bacteria and kleptoplast in Virgulinella fragilis (foraminifera) : distribution, cytological and genetic characteristics (2010)
  • Author(s): Tsuchiya, M., Toyofuku, T., Uematsu, K., Bruchert, V., Collen, J., Yamamoto, H., Kitazato, H.
  • Organizer: Memorial Symposium for the 26th Internation al Prize for Biology
  • Place of Presentation: つくば国際会議場、茨城県つくば市
  • Year and Date: 2010-12-07
• [Presentation] Feeding strategies among deepsea benthic foraminifera: Evidences from TEM observations and in-situ experiments for modern deep-sea foraminifera (2010)
  • Author(s): Kitazato, H., Nomaki, H., Toyofuku, T
  • Organizer: International Symposium on Foraminifera FORAMS2010
  • Place of Presentation: Bonn, Germany
  • Year and Date: 2010-09-06
• [Presentation] 2-D pH observation on foraminiferal cell under stable environmental condition (2010)
  • Author(s): Toyofuku, T., de Nooijer, L. J., Nomaki, H., Tsuchiya, M., Kitazato, H
  • Organizer: International Symposium on Foraminifera FORAMS2010
  • Place of Presentation: Bonn, Germany
  • Year and Date: 2010-09-06
• [Presentation] 2-D pH observation on foraminiferal cell under stable environmental condition (2010)
  • Author(s): Toyofiiku T, de Nooijer LJ, Nomaki H, Tsuchiya M, Schiebel M, Kitazato H
  • Organizer: FORAMS2010
  • Place of Presentation: ボン・ドイツ
  • Year and Date: 2010-09-06
• [Presentation] Onboard and in situ incubation experiments for understanding deep-sea benthic foraminiferal ecologies (2010)
  • Author(s): Nomaki H, Toyofuku T, de Nooijer LJ, Suga H, Kitazato H
  • Organizer: FORAMS2010
  • Place of Presentation: ボン・ドイツ
  • Year and Date: 2010-09-06
• [Presentation] 石灰質有孔虫の殻内タンパク質の検討 (2010)
  • Author(s): 更科 功, 豊福高志, 藤田和彦, 遠藤一佳
  • Organizer: 日本古生物学会2010年年会
  • Place of Presentation: 筑波大学
  • Year and Date: 2010-06-12
• [Presentation] Utilization of dissolved organic matter by the deep-sea benthic community at the Sagami Bay, Japan (2010)
  • Author(s): Nomaki H, Ogawa NO, Takano Y, Suga H, Ohkouchi N, Toyofuku T, Kitazato H
  • Organizer: 12th Deep-Sea Biology Symposium
  • Place of Presentation: レイキャビク・アイスランド
  • Year and Date: 2010-06-10
• [Presentation] 石灰質有孔虫細胞内環境と石灰化過程 (2010)
  • Author(s): 豊福高志、レナート=ヤン=ド・ノーイエ、野牧秀隆、小栗一将、土屋正史、北里洋
  • Organizer: 日本地球惑星科学連合2010年大会
  • Place of Presentation: 幕張メッセ、千葉市
  • Year and Date: 2010-05-24
• [Presentation] Intracellular environment of carcaleous foraminifera and biomineralization (2010)
  • Author(s): Toyofuku, T., de Nooijer, L.J., Nomaki, H., Oguri, K., Tsuchiya, M., Kitazato, H
  • Organizer: Japan Geoscience Union Meeting 2010
  • Place of Presentation: Makuhari Messe, Chiba, Japan
• [Presentation] study of test reconstruction of Ammonia beccarii for extreme environmental conditions (2010)
  • Author(s): Asai, T., Toyofuku, T., Nomaki, H., Miwa, T
  • Organizer: 極限環境生物学会
  • Place of Presentation: 京都大学宇治キャンパス
• [Presentation] Pressure effect on living shallow water benthic foraminifera (2010)
  • Author(s): Asai, T., Toyofuku, T., Nomaki, H
  • Organizer: 日本地球惑星科学連合2010年大会
  • Place of Presentation: 幕張メッセ国際会議場
• [Presentation] Deep-sea benthic food-web structure at the bathyal Sagami Bay revealed by in situ experiments and linear inverse model (2010)
  • Author(s): Nomaki H, van Oevelen D, Toyofuku T, Kitazato H
  • Organizer: Trench Connection International Symposium on the Deepest Environment on Earth
  • Place of Presentation: 東京大学大気海洋研究所、千葉県柏市
• [Remarks] JAMSTEC研究者個人ページ
  • URL: http://www.jamstec.go.jp/seika/pub-j/res/ress/toyofuku/index.html
• [Remarks] TWITTER
  • URL: https://twitter.com/t_toyofuku
• [Remarks] タンブラー
  • URL: http://toyofuku.tumblr.com/
• [Remarks] FACEBOOK
  • URL: https://www.facebook.com/toyohuka
• [Remarks] (1)Toyofuku, T. How the natural time machines are prepared? ?some laboratory works on foraminiferal calcification. Lund University, Sweden, 27 Nov. 2012. セミナー&集中講義
• [Remarks] (2)豊福高志 福島大学集中講義. 2012年11月14日. セミナー&集中講義
• [Remarks] (3)豊福高志 浜松修学舎高等学校研究紹介, 2012年11月12日. セミナー&集中講義
• [Remarks] (4)豊福高志 熊本大学集中講義. 2012年10月17日, 18日. セミナー&集中講義
• [Remarks] (5)豊福高志 Foraminiferal intracellular environmental observation ?perspective for biomineralization, geochemical proxy for past time ocean and ocean acidification.バイオミネラリゼーションセミナー,東京大学農学部2号館,2012年9月24日. セミナー&集中講義
• [Remarks] (6)豊福高志 サイエンスパートナーシッププロジェクト講座. 宇都宮高等学校, 2012年9月11日. セミナー&集中講義
• [Remarks] (7)豊福高志 横浜国立大学集中講義. 2012年7月31日?8月3日. セミナー&集中講義
• [Remarks] (8)豊福高志 海洋酸性化を想定した実内実験, JpGU JAMSTECブース. JpGU2011, 幕張メッセ, 2011年5月25日. セミナー&集中講義
• [Remarks] (9)豊福高志 Mg/Ca in calcareous foraminifera from laboratory culture experiment. 熊本大学セミナー, 2011年3月10日. セミナー&集中講義
• [Remarks] (10)Toyofuku, T. Revealing Foraminiferal Calcification and Its Impact on trace elemental incorporation. Alfred Wegener Institute, Bremerhaven, Germany, 1st., September 2010. セミナー&集中講義
• [Remarks] (11)Toyofuku, T. Revealing Foraminiferal Calcification and Its Impact on trace elemental incorporation. Utrecht University, Utrecht, Netherlands, 30th, August 2010. セミナー&集中講義
• [Remarks] Toyofuku, T. Intracellular environment of calcareous foraminifera and biomineralization (Invited seminar). Anger University, Anger, France, 23^<rd>, June 2010.
• [Remarks] 地球生物学研究チーム
  • URL: http://www.jamstec.go.jp/biogeos/j/elhrp/geobiology/
• [Remarks] Twitter (Takashi Toyofuku)
  • URL: https://twitter.com/t_toyofuku
• [Remarks] JAMSTEC機関リポジトリ
  • URL: http://www.jamstec.go.jp/jir/infolib/meta_pub/search-jp
• [Remarks]
  • URL: http://www.jamstec.go.jp/biogeos/j/elhrp/geobiology/index.html
• [Remarks]
  • URL: http://www.jamstec.go.jp/biogeos/j/elhrp/geobiology/index.html