| Budget Amount *help |
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2010: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
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| Research Abstract |
The research objective of this project was to investigate the deep-sea benthic archaea, sub-seafloor unseen major prokaryote, and their biogeochemical process by a novel stable isotope technology from insight of metabolomics and lipidomics. Since most benthic archaea are viable but non-culturable microbes and difficult to study in the laboratory, we conducted in situ <13>^C-tracer experiments 0-405 days at Sagami Bay, Western Pacific. We found that the <13>^C was peculiarly enriched into the 2,3-sn-glycerol backbone of archaeal membranes, while the isoprenoid chain remained unlabelled. 16S rRNA and quantitative PCR (qPCR) analysis indicated a community shift in the composition of the community and its abundance. We suggest that benthic archaea build their membranes by recycling relic lipids via salvage pathway to minimize the energy expenditure for de novo synthesis.
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