Deep-sea benthic archaea: unseen major prokaryote and their biogeochemical process
Project/Area Number |
22684030
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Geochemistry/Astrochemistry
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Research Institution | Japan Agency for Marine-Earth Science and Technology |
Principal Investigator |
TAKANO Yoshinori 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (80399815)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2010: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
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Keywords | Deep Biosphere / 生物圏地球化学 / 分子レベル同位体 / 生物地球化学循環 / 海底下の地下生物圏 / 性状未知アーキア / 生物地球化学プロセス / Stable Isotope Probing法 / Salvage pathway / de novo pathway / 性状未知なdeep-sea benthic archaea / 特異な代謝 / Stable Isotope Probing法(SIP法) / Stable lsotope Probing法(SIP法) |
Research Abstract |
The research objective of this project was to investigate the deep-sea benthic archaea, sub-seafloor unseen major prokaryote, and their biogeochemical process by a novel stable isotope technology from insight of metabolomics and lipidomics. Since most benthic archaea are viable but non-culturable microbes and difficult to study in the laboratory, we conducted in situ <13>^C-tracer experiments 0-405 days at Sagami Bay, Western Pacific. We found that the <13>^C was peculiarly enriched into the 2,3-sn-glycerol backbone of archaeal membranes, while the isoprenoid chain remained unlabelled. 16S rRNA and quantitative PCR (qPCR) analysis indicated a community shift in the composition of the community and its abundance. We suggest that benthic archaea build their membranes by recycling relic lipids via salvage pathway to minimize the energy expenditure for de novo synthesis.
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Report
(4 results)
Research Products
(87 results)
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[Journal Article] Detection of coenzyme F430 in deep-sea sediments: A key molecule for biological methanogenesis2013
Author(s)
Takano, Y., Kaneko, M., Kahnt, J., Imachi, H., Shima, S. and Ohkouchi, N
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Journal Title
Organic Geochemistry
Volume: (in press)
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Peer Reviewed
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[Journal Article] Detection of coenzyme F430 in deep-sea sediments: A key molecule for biological methanogenesis.2013
Author(s)
Takano, Y., Kaneko, M., Kahnt, J., Imachi, H., Shima, S. and Ohkouchi, N.
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Journal Title
Organic Geochemistry
Volume: in press
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Peer Reviewed
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[Journal Article] Holocene lake development and glacial-isostatic uplift at Lake Skallen and Lake Oyako, Lu-tzow-Holm Bay, East Antarctica : based on biogeocheaical facies and molecular signatures.2012
Author(s)
Takano Y, Tyler JJ, Kojima H, Yokoyama Y, Tanabe Y, Sato T, Ogawa ON, Ohkouchi N, Fukui M
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Journal Title
Applied Geochemistry
Volume: 27
Issue: 12
Pages: 2546-2559
DOI
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Peer Reviewed
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[Journal Article] Characterization of Organic Aggregates Formed by Heating Products of Simulated Primitive Earth Atmosphere2012
Author(s)
Kurihara, H., Yabuta, H., Kaneko, T., Obayashi, Y., Takano, Y. and Kobayashi, K.
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Journal Title
Chem. Lett.
Volume: 41
Issue: 4
Pages: 441-443
DOI
NAID
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Peer Reviewed
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[Journal Article] Stability of Amino Acids and Related Compounds in Simulated Submarine Hydrothermal Systems2012
Author(s)
Kurihara, H., Takano, Y., Kaneko, T., Obayashi, Y., and Kobayashi, K.
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Journal Title
Bulletin of the Chemical Society of Japan
Volume: 85
Issue: 5
Pages: 624-630
DOI
NAID
ISSN
0009-2673, 1348-0634
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Peer Reviewed
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[Journal Article] Differing utilization of glucose and algal particulate organic matter by the deep-sea benthic organisms of Sagami Bay2011
Author(s)
Nomaki, H., Ogawa, O.N., Takano, Y., Suga, H., Ohkouchi, N., Kitazato, H
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Journal Title
Japan. Marine Ecology Progress Series
Volume: 431
Pages: 11-24
DOI
Related Report
Peer Reviewed
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[Journal Article] Geophysical and geochemical evidence of large scale fluid flow within shallow sediments in the eastern Gulf of Mexico2011
Author(s)
Gay, A., Takano, Y., Gilhooly III, W.P., Berndt, C., Heeschen, K., Suzuki, N., Saegusa, S., Nakagawa, F., Tsunogai, U., Jiang, S.Y., and Lopez, M
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Journal Title
offshore Louisiana. Geofluids
Volume: 11
Issue: 1
Pages: 34-47
DOI
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Peer Reviewed
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[Journal Article] Sedimentary membrane lipids recycled by deep-sea benthic archaea2010
Author(s)
Takano, Y., Chikaraishi, Y., Ogawa, O.N., Nomaki, H., Morono, Y., Inagaki, F., Kitazato, H., Hinrichs, K.-U., Ohkouchi, N
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Journal Title
Nature Geoscience
Volume: 3
Pages: 858-861
NAID
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Peer Reviewed
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[Journal Article] Crustal uplifting rate associated with late-Holocene glacial-isostatic rebound at Skallen and Skarvsnes, Lutzow-Holm Bay, East Antarctica: evidence of a synchrony in sedimentary and biological facies on geological setting2010
Author(s)
Takano, Y., Yokoyama, Y., Tyler, J.J., Fukui, M., Sato, T., Ogawa, N.O., Suzuki, N., Kitazato, H., Ohkouchi, N
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Journal Title
Biogeosciences Discuss
Volume: 7
Pages: 4341-4384
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Peer Reviewed
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[Journal Article] Microbial community structure, pigment composition, and nitrogen source of red snow in Antarctica2010
Author(s)
Fujii, M., Takano, Y., Kojima, H., Hoshino, T., Tanaka, R., and Fukui, M
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Journal Title
Microbial Ecology
Volume: 59
Issue: 3
Pages: 466-475
DOI
NAID
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Peer Reviewed
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[Journal Article] Biogeography and biodiversity in sulfide structures of active and inactive vents at deep-sea hydrothermal fields of the Southern Mariana Trough2010
Author(s)
Kato, S., Takano, Y., Kakegawa, T., Oba, H., Inoue, K., Kobayashi, C., Utsumi, M., Marumo, K., Kobayashi, K., Ito, Y., Ishibashi, J., and Yamagishi, A
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Journal Title
Applied and Environmental Microbiology
Volume: 76
Issue: 9
Pages: 2968-2979
DOI
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Peer Reviewed
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[Book] High molecular weight complex organics in interstellar space and their relevance to origins of life. Astrobiology: Emergence, Search and Detection of Life (edited by V. Basiuk)2010
Author(s)
Kobayashi, K., Kaneko, T., Takahashi, J.-I., Takano, Y., and Yoshida, S
Publisher
American Scientific Publishers
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