| Budget Amount *help |
¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2010: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Research Abstract |
In this study, we have developed measurement techniques for electrokinetic flow dynamics in nano-confiend spaces and applied our techniques to develop novel techniques for the analyses of biomacromolecules. We first investigated electrophoretic flows of biomacromolecules in nano channels and nano pores, and found the nano-confinement decreases the mobility of the biomacromolecules. We second extended our measurement technique to investigate unsteady ionic flows in the cell electroporation with micro-pores. We lastly proposed a technique for simultaneously extracting, separating, and quantifying RNA and DNA from single cells by integrating on-chip electroporation and isotachophoresis.
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