Visualization techniques for electrokinetic flow dynamics in confined nanospace
| Project/Area Number |
22686021
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fluid engineering
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| Research Institution | Kyoto University (2012-2013)
Osaka University (2010-2011) |
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Principal Investigator |
SHINTAKU Hirofumi 京都大学, 工学(系)研究科(研究院), 助教 (80448050)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2010: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Keywords | 界面動電現象 / ナノ制限空間 / 生体高分子 / 1分子計測 / MEMS流体 / 一分子計測 / マイクロ・ナノ流体 / 可視化 / MEMS / DNAシーケンシング |
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| Research Abstract |
In this study, we have developed measurement techniques for electrokinetic flow dynamics in nano-confiend spaces and applied our techniques to develop novel techniques for the analyses of biomacromolecules. We first investigated electrophoretic flows of biomacromolecules in nano channels and nano pores, and found the nano-confinement decreases the mobility of the biomacromolecules. We second extended our measurement technique to investigate unsteady ionic flows in the cell electroporation with micro-pores. We lastly proposed a technique for simultaneously extracting, separating, and quantifying RNA and DNA from single cells by integrating on-chip electroporation and isotachophoresis.
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Report
(5 results)
Research Products
(49 results)
