Cell selection based on genetic signal
| Project/Area Number |
22686077
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
ABE Hiroshi 独立行政法人理化学研究所, 伊藤ナノ医工学研究室, 専任研究員 (80415067)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
Fiscal Year 2011: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2010: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
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| Keywords | RNA / 細胞 / 分離 / イメージング / 蛍光 / 化学反応 / フローサイトメーター / DNA / 未分化 / 検出 / miRNA / 遺伝子 / 還元反応 / 単離 / プローブ |
|---|
| Research Abstract |
Our aim is to develop new RNA detection probe which is capable of cell selection based on genetic signal. First, we synthesized red fluorescent probe to offer very high signal and background ratio. Next, the probe was designed to target miRNA which is expressed in stem cells. As a result, we confirmed specific miRNA signal in model cells. Now, we are trying to isolate cells with specific miRNA signal.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Universal Caging Group for In Cell Detection of Glutathione Transferase Applied to 19F NMR and Bioluminogenic Probes2012
Author(s)
Mika Ito, Aya Shibata, Jie Zhang, Michio Hiroshima, Yasushi Sako, Yukiko Nakano, Kyoko Kojima-Aikawa, Bengt Mannervik, Satoshi Shuto, Yoshihiro Ito, Ralf Morgenstern, Hiroshi Abe
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Journal Title
ChemBioChem
Volume: (in press)
Related Report
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[Journal Article] Ralf Morgenstern, Characterization of New Potential Anticancer Drugs Designed To Overcome Glutathione Transferase Mediated Resistance2011
Author(s)
Katarina Johansson, Mika Ito, Carolien M. S. Schophuizen, Sherin Mathew T, Vanina D. Heuser, Jie Zhang, Miyuki Shimoji, Marie Vahter, Wee Han Ang, Paul J. Dyson, Aya Shibata, Satoshi Shuto, Yoshihiro, Ito, Hiroshi Abe
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Journal Title
Molecular Pharmaceutics.
Volume: 8(5)
Pages: 1698-1708
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