Identification of positional signals regulating shoot epidermal cell fate

• Project/Area Number: 22687003
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Osaka University
• Principal Investigator: TAKADA Shinobu 大阪大学, 理学(系)研究科(研究院), 助教 (40456992)
• Research Collaborator: TAKADA Nozomi ; YOSHIDA Ayaka ; IIDA Hiroyuki ; ITO Miharu
• Project Period (FY): 2010-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000); Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2010: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: 細胞分化 / 位置情報 / パターン形成 / 胚発生 / 転写制御 / シロイヌナズナ / 胚 / 転写 / 植物

Outline of Final Research Achievements

Cell-type specific transcription factors play key roles in determining cell fate through the regulation of gene expression. However, molecular mechanisms regulating the cell-type dependent activity of transcriptional factors are largely unknown. Our studies showed that ATML1 functions as a master transcriptional regulator for shoot epidermal cell fate in Arabidopsis thaliana. We developed new tools to identify key components that restrict ATML1 expression to the outermost cell layer.

Research Products

• [Journal Article] Specification of epidermal cell fate in plant shoots (2014)
  • Author(s): Shinobu Takada and Hiroyuki Iida
  • Journal Title: Frontiers in Plant Science Volume: 5 Pages: 49-49
  • DOI: 10.3389/fpls.2014.00049
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ATML1 promotes epidermal cell differentiation in Arabidopsis shoots (2013)
  • Author(s): Shinobu Takada, Nozomi Takada, and Ayaka Yoshida
  • Journal Title: Development Volume: 140 Issue: 9 Pages: 1919-1923
  • DOI: 10.1242/dev.094417
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Induction of epidermal cell fate in Arabidopsis shoots (2013)
  • Author(s): Shinobu Takada, Nozomi Takada, and Ayaka Yoshida
  • Journal Title: Plant Signaling & Behavior Volume: 8 Issue: 11 Pages: e26236-e26236
  • DOI: 10.4161/psb.26236
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Post-embryonic induction of ATML1-SRDX alters the morphology of seedlings (2013)
  • Author(s): Shinobu Takada
  • Journal Title: PLoS ONE Volume: 8 Issue: 10 Pages: e79312-e79312
  • DOI: 10.1371/journal.pone.0079312
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Efficient Yeast One-/Two-Hybrid Screening Using a Library Composed Only of Transcription Factors in Arabidopsis thaliana (2010)
  • Author(s): Nobutaka Mitsuda, Miho Ikeda, Shinobu Takada, Yuko Takiguchi, Youichi Kondou, Takeshi Yoshizumi, Miki Fujita, Kazuo Shinozaki, Minami Matsui, Masaru Ohme-Takagi
  • Journal Title: Plant & Cell Physiology Volume: 51 Pages: 2145-2151
  • NAID: 10028056951
  • Peer Reviewed
• [Presentation] Transcriptional regulation of epidermal cell fate in Arabidopsis thaliana shoots (2014)
  • Author(s): Hiroyuki Iida, Nozomi Takada, Ayaka Yoshida, and Shinobu Takada
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学五福キャンパス
  • Year and Date: 2014-03-20
• [Presentation] ATML1 activates the expression of ACR4 during initiation of epidermal cell fate (2014)
  • Author(s): Nozomi Takada, Ayaka Yoshida, and Shinobu Takada
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学五福キャンパス
  • Year and Date: 2014-03-18
• [Presentation] ARABIDOPSIS CRINKLY 4 is a downstream target of ATML1-mediated transcriptional regulation (2013)
  • Author(s): Nozomi Takada, Ayaka Yoshida, and Shinobu Takada
  • Organizer: 24th International Conference on Arabidopsis Research
  • Place of Presentation: Sydney Convention and Exhibition Centre, Sydney, Australia
  • Year and Date: 2013-06-25
• [Presentation] ATML1 promotes epidermal cell differentiation in Arabidopsis shoots (2013)
  • Author(s): Shinobu Takada, Nozomi Takada, and Ayaka Yoshida
  • Organizer: 24th International Conference on Arabidopsis Research
  • Place of Presentation: Sydney Convention and Exhibition Centre, Sydney, Australia
  • Year and Date: 2013-06-25
• [Presentation] ATML1 promotes epidermal cell differentiation in the post-embryonic development (2013)
  • Author(s): 高田忍、高田 希、吉田彩香
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] Regulation of epidermis-specific expression of ATML1 (2013)
  • Author(s): 吉田彩香、Gerd Juergens、高田忍
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] シロイヌナズナのATML1遺伝子は表皮分化を正に制御する (2012)
  • Author(s): 高田忍、吉田彩香、高田 希
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] ATML1の表皮特異的な発現におけるautoregulationの役割 (2012)
  • Author(s): 吉田彩香、Gerd Juergens、高田忍
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] ATML1 regulates epidermal cell differentiation in Arabidopsis shoots (2012)
  • Author(s): 高田忍、高田希、吉田彩香
  • Organizer: 23rd International Conference on Arabidopsis Research
  • Place of Presentation: Hofburg Imperial Palace, Vienna, Austria
• [Presentation] ATML1 regulates epidermal cell differentiation in Arabidopsis shoots (2012)
  • Author(s): 高田忍、吉田彩香
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
• [Presentation] シロイヌナズナのATML1遺伝子は表皮特異的な遺伝子の発現を正に制御する (2011)
  • Author(s): 高田忍、吉田彩香
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-22
• [Presentation] ATML1の表皮特異的な発現を決める遺伝子の探索 (2011)
  • Author(s): 吉田彩香、Gerd Jurgens、高田忍
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-20
• [Presentation] Transcriptional regulation of epidermis-specific gene expression (2011)
  • Author(s): 高田忍、吉田彩香
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] シロイヌナズナのATML1遺伝子は表皮特異的な遺伝子の発現を正に制御する (2011)
  • Author(s): 高田忍、吉田彩香
  • Organizer: 第75回大会日本植物学会
  • Place of Presentation: 東大駒場キャンパス
• [Presentation] ATML1の表皮特異的な発現を決める遺伝子の探索 (2011)
  • Author(s): 吉田彩香、Gerd Jürgens、高田忍
  • Organizer: 第75回大会日本植物学会
  • Place of Presentation: 東大駒場キャンパス
• [Presentation] Transcriptional regulation of epidermis-specific gene expression (2010)
  • Author(s): 高田忍、柿本辰男、Gerd Jurgens
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-06-07
• [Remarks] 植物胚発生のページ
  • URL: http://www.bio.sci.osaka-u.ac.jp/~shinobu_takada/index.html
• [Remarks] 世界初 植物の別細胞から表皮細胞を作成することに成功
  • URL: http://resou.osaka-u.ac.jp/ja/research/2013/20130408_1
• [Remarks] 植物マスター遺伝子を解明、葉の内部に表皮の組織を作成 阪大が成功
  • URL: http://sankei.jp.msn.com/west/west_life/news/130410/wlf13041016310020-n1.htm
• [Remarks] 世界初 植物の別細胞から表皮細胞を作成することに成功 植物の進化の過程や形づくりの理解促進へ
  • URL: http://www.osaka-u.ac.jp/ja/news/ResearchRelease/2013/04/20130408_1
• [Remarks] 植物胚発生研究のページ
  • URL: http://www.bio.sci.osaka-u.ac.jp/~shinobu_takada/index.html
• [Remarks] 植物胚パターン形成のページ（高田忍のページ）
  • URL: http://www.bio.sci.osaka-u.ac.jp/~shinobu_takada/index.html
• [Remarks]
  • URL: http://www.bio.sci.osaka-u.ac.jp/~shinobu_takada/index.html