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Study on physiological function of a novel food intake-regulatory hypothalamic gene and its transcript

Research Project

Project/Area Number 22687004
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Morphology/Structure
Research InstitutionHiroshima University

Principal Investigator

UKENA Kazuyoshi  広島大学, 総合科学研究科, 准教授 (10304370)

Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2010: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords神経ペプチド / 視床下部 / 摂食調節 / 新規遺伝子 / ペプチド同定 / 摂食行動 / 構造解析 / 組換えタンパク質
Research Abstract

Recently, we have identified a novel hypothalamic gene which encodes a neuropeptide in the avian and mammalian brains. As the mRNA expression was changed during different energy states such as fasting and obesity, we predict that this novel gene may be involved in the energy homeostasis, especially food intake. In this study, we investigated the following analyses, identification of mature endogenous peptide, effect on food intake, histological relationships with known substances, regulatory mechanisms by peripheral substances, and phylogenetic analysis.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (54 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (24 results) (of which Peer Reviewed: 24 results,  Open Access: 2 results) Presentation (24 results) (of which Invited: 1 results) Remarks (1 results) Patent(Industrial Property Rights) (5 results)

  • [Journal Article] MOLECULAR EVOLUTION OF GPCRS : 26Rfa/GPR1032014

    • Author(s)
      Ukena K, Osugi T, Leprince J, Vaudry V, Tsutsui K
    • Journal Title

      J. Mol. Endocrinol

      Volume: 52

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a cDNA encoding a novel small secretory protein, neurosecretory protein GL, in the chicken hypothalamic infundibulum2014

    • Author(s)
      Ukena K, Iwakoshi-Ukena E, Taniuchi S, Bessho Y, Maejima S, Masuda K, Shikano K, Kondo K, Furumitsu M, Tachibana T.
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 446 Pages: 298-303

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of neurotensin and LANT-6 and localization of mRNA encoding their precursor in the chicken brain2014

    • Author(s)
      Masuda K, Iwakoshi-Ukena E, Bessho Y, Taniuchi S, Maejima S, Shikano K, Kondo K, Furumitsu M, Ukena K
    • Journal Title

      Zool. Sci

      Volume: 31 Pages: 353-359

    • NAID

      40020091008

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a cDNA encoding a novel small secretory protein, neurosecretory protein GL, in the chicken hypothalamic infundibulum.2014

    • Author(s)
      Ukena K, Iwakoshi-Ukena E, Taniuchi S, Bessho Y, Maejima S, Masuda K, Shikano K, Kondo K, Furumitsu M, Tachibana T.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 446 Issue: 1 Pages: 298-303

    • DOI

      10.1016/j.bbrc.2014.02.090

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular evolution and function of 26RFa/QRFP and its cognate receptor.2014

    • Author(s)
      Ukena K, Osugi T, Leprince J, Vaudry V, Tsutsui K.
    • Journal Title

      J. Mol. Endocrinol.

      Volume: in press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of neurotensin and LANT-6 and localization of mRNA encoding their precursor in the chicken brain.2014

    • Author(s)
      Masuda K, Iwakoshi-Ukena E, Bessho Y, Taniuchi S, Maejima S, Shikano K, Kondo K, Furumitsu M, Ukena K.
    • Journal Title

      Zoolical Science

      Volume: 31 Issue: 6 Pages: 353-359

    • DOI

      10.2108/zs140010

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification, localization and function of a novel neuropeptide, 26RFa, and its cognate receptor, GPR103, in the avian hypothalamus2013

    • Author(s)
      Ukena K, Tachibana T, Tobari Y, Leprince J, Vaudry H, Tsutsui K
    • Journal Title

      Gen. Comp. Endocrinol

      Volume: 190 Pages: 42-46

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ニワトリにおいて視床下部漏斗部に特異的発現を示す新規遺伝子の同定2013

    • Author(s)
      岩越栄子、橘 哲也、谷内秀輔、古満芽久美、益田恵子、浮穴和義
    • Journal Title

      日本家禽学会誌

      Volume: 50 Pages: 10-15

    • NAID

      10031167911

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Review: Identification, localization and function of a novel neuropeptide, 26RFa, and its cognate receptor, GPR103 in the avian hypothalamus2013

    • Author(s)
      K. Ukena, T. Tachibana, Y. Tobari, J. Leprince, H. Vaudry, K. Tsutsui
    • Journal Title

      Gen. Comp. Endocrinol.

      Volume: 190 Pages: 42-46

    • DOI

      10.1016/j.ygcen.2013.03.014

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification, expression, and physiological functions of siberian hamster gonadotropin-inhibitory hormone2012

    • Author(s)
      Ubuka T, Inoue K, Fukuda Y, Mizuno T, Ukena K, Kriegsfeld LJ, Tsutsui K
    • Journal Title

      Endocrinology

      Volume: 153 Pages: 373-385

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification, expression, and physiological functions of Siberian hamster gonadotropin-inhibitory hormone2012

    • Author(s)
      T.Ubuka, K.Tsutsui, et al
    • Journal Title

      Endocrinology

      Volume: 153 Issue: 1 Pages: 373-385

    • DOI

      10.1210/en.2011-1110

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular evolution and functional characterization of the orexigenic peptide 26RFa and its receptor in vertebrates2011

    • Author(s)
      Ukena K, Vaudry H, Leprince J, Tsutsui K
    • Journal Title

      Cell Tissue Res

      Volume: 343 Pages: 475-481

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and structure-activity relationship of an antimicrobial peptide of the palustrin-2 family isolated from the skin of the endangered frog Odorrana ishikawae2011

    • Author(s)
      Iwakoshi-Ukena E, Okada G, Okimoto A, Fujii T, Sumida M, Ukena K
    • Journal Title

      Peptides

      Volume: 32 Pages: 2052-2057

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of novel antimicrobial peptides from the skin of the endangered frog Odorrana ishikawae by shotgun cDNA cloning2011

    • Author(s)
      Iwakoshi-Ukena E, Soga M, Okada G, Fujii T, Sumida M, Ukena K
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 412 Pages: 673-677

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of antimicrobial peptides from the skin of the endangered frog Odorrana ishikawae2011

    • Author(s)
      Iwakoshi-Ukena E, Ukena K, Okimoto A, Soga M, Okada G, Sano N, Fujii T, Sugawara Y, Sumida M
    • Journal Title

      Peptides

      Volume: 32 Pages: 670-676

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synchronised expressions of LPXRFamide peptide and its receptor genes : seasonal, diurnal and circadian changes during spawning period in grass puffer2011

    • Author(s)
      Shahjahan Md, Ikegami T, Osugi T, Ukena K, Doi H, Hattori A, Tsutsui K, Ando H
    • Journal Title

      J. Neuroendocrinol

      Volume: 23 Pages: 39-51

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification, localisation and functional implication of 26RFa orthologue peptide in the brain of zebra finch (Taeniopygia guttata)2011

    • Author(s)
      Tobari Y, Iijima N, Tsunekawa K, Osugi T, Haraguchi S, Ubuka T, Ukena K, Okanoya K, Tsutsui K, Ozawa H
    • Journal Title

      J. Neuroendocrinol

      Volume: 23 Pages: 791-803

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis, mode of action and functional significance of neurosteroids in the Purkinje cell2011

    • Author(s)
      Kazuyoshi Tsutsui
    • Journal Title

      Frontiers in Endocrinology

      Volume: 2 Pages: 1-9

    • DOI

      10.3389/fendo.2011.00061

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of novel antimicrobial peptides from the skin of the endangered frog Odorrana ishikawae by shotgun cDNA cloning2011

    • Author(s)
      Iwakoshi-Ukena E
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 412 Issue: 4 Pages: 673-677

    • DOI

      10.1016/j.bbrc.2011.08.023

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification, Localisation and Functional Implication of 26RFa Orthologue Peptide in the Brain of Zebra Finch (Taeniopygia guttata)2011

    • Author(s)
      Y.Tobari, K.Tsutsui, et al
    • Journal Title

      J.Neuroendocrinol.

      Volume: 23 Issue: 9 Pages: 791

    • DOI

      10.1111/j.1365-2826.2011.02179.x

    • URL

      https://pure.teikyo.jp/en/publications/70eda0c3-6213-4976-a722-9876ed8e2497

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synchronised expressions of LPXRFamide peptide and its receptor genes : seasonal, diurnal and circadian changes during spawning period in grass puffer.2011

    • Author(s)
      Shahjahan Md
    • Journal Title

      J.Neuroendocrinol.

      Volume: 23 Pages: 39-51

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular evolution and functional characterization of the orexigenic peptide 26RFa and its receptor in vertebrates.2011

    • Author(s)
      Ukena K
    • Journal Title

      Cell Tissue Res.

      Volume: 343 Pages: 475-481

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification, localization, and function of a novel avian hypothalamic neuropeptide, 26RFa, and its cognate receptor, G protein-coupled receptor-1032010

    • Author(s)
      Ukena K, Tachibana T, Iwakoshi-Ukena E, Saito Y, Minakata H, Kawaguchi R, Osugi T, Tobari Y, Leprince J, Vaudry H, Tsutsui K
    • Journal Title

      Endocrinology

      Volume: 151 Pages: 2255-2264

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification, localization, and function of a novel avian hypothalamic neuropeptide, 26RFa, and its cognate receptor, G protein-coupled receptor-103.2010

    • Author(s)
      Ukena K
    • Journal Title

      Endocrinology

      Volume: 151 Pages: 2255-2264

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 視床下部で見出した新規神経ペプチド前駆体遺伝子の進化的保存性2013

    • Author(s)
      谷内秀輔, 岩越-浮穴栄子, 浮穴和義
    • Organizer
      第84回日本動物学会大会
    • Place of Presentation
      岡山
    • Year and Date
      2013-09-27
    • Related Report
      2013 Final Research Report
  • [Presentation] 視床下部新規遺伝子がコードしている成熟神経ペプチドの同定2012

    • Author(s)
      岩越栄子, 古満芽久美, 谷内秀輔, 別所裕紀, 浮穴和義
    • Organizer
      第37回日本比較内分泌学会大会
    • Place of Presentation
      福井
    • Year and Date
      2012-11-30
    • Related Report
      2013 Final Research Report
  • [Presentation] ラットの視床下部新規神経ペプチドの形態学的解析-既知の生理活性物質との相関と絶食処理に伴う変化-2012

    • Author(s)
      前嶋 翔, 岩越栄子, 佐藤慧太, 坂本浩隆, 坂本竜哉, 浮穴和義
    • Organizer
      第37回日本比較内分泌学会大会
    • Place of Presentation
      福井
    • Year and Date
      2012-11-30
    • Related Report
      2013 Final Research Report
  • [Presentation] ラットの視床下部新規遺伝子がコードしている神経ペプチドの摂食行動への影響2012

    • Author(s)
      鹿野健史朗, 近藤邦裕, 谷内秀輔, 大口悦宏, 大山晴香, 益田恵子, 前嶋 翔, 岩越栄子, 浮穴和義
    • Organizer
      第37回日本比較内分泌学会大会
    • Place of Presentation
      福井
    • Year and Date
      2012-11-30
    • Related Report
      2013 Final Research Report
  • [Presentation] 視床下部で発見した新規遺伝子は分泌性ペプチドの前駆体か?2012

    • Author(s)
      古満芽久美, 岩越-浮穴栄子, 谷内秀輔, 浮穴和義
    • Organizer
      第83回日本動物学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2012-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] ニワトリ視床下部で発見した新規遺伝子がコードしている神経ペプチドの機能解析2012

    • Author(s)
      谷内秀輔, 岩越-浮穴栄子, 古満芽久美, 橘 哲也, 浮穴和義
    • Organizer
      第83回日本動物学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2012-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] ラットの視床下部新規遺伝子がコードしている神経ペプチドの形態学的解析2012

    • Author(s)
      前嶋 翔, 佐藤真実, 岩越-浮穴栄子, 浮穴和義
    • Organizer
      第83回日本動物学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2012-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 鳥類の視床下部漏斗部で発見した新規遺伝子の機能解析-摂食調節機構に着目して-2011

    • Author(s)
      浮穴和義
    • Organizer
      第38回日本神経内分泌学会学術集会
    • Place of Presentation
      東京都(招待講演)
    • Year and Date
      2011-11-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 哺乳類培養細胞を用いた新規摂食調節関連遺伝子がコードしている神経ペプチドの同定2011

    • Author(s)
      岩越-浮穴栄子, 山崎玲子, 古満芽久美, 斎藤祐見子, 浮穴和義
    • Organizer
      第82回日本動物学会大会
    • Place of Presentation
      旭川
    • Year and Date
      2011-09-21
    • Related Report
      2013 Final Research Report
  • [Presentation] ラットの新規摂食調節関連遺伝子がコードしている神経ペプチドによる摂食行動の解析2011

    • Author(s)
      大口悦宏, 佐藤真実, 古満芽久美, 益田恵子, 岩越栄子, 浮穴和義
    • Organizer
      第36回日本比較内分泌学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] ニワトリの新規摂食調節関連遺伝子がコードしている神経ペプチドの摂食行動に及ぼす影響2011

    • Author(s)
      岩越栄子, 橘哲也, 古満芽久美, 浮穴和義
    • Organizer
      第36回日本比較内分泌学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] 視床下部における新規摂食調節関連遺伝子の発見2010

    • Author(s)
      岩越栄子, 田中幸恵, 橘 哲也, 浮穴和義
    • Organizer
      第35回日本比較内分泌学会大会
    • Place of Presentation
      静岡
    • Year and Date
      2010-11-20
    • Related Report
      2013 Final Research Report
  • [Presentation] ニワトリの視床下部で見出した新規摂食調節関連遺伝子2010

    • Author(s)
      浮穴和義
    • Organizer
      第81回日本動物学会大会
    • Place of Presentation
      東京都
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] ニワトリで発見した新規神経ペプチドはヒスタミンニューロンに発現している

    • Author(s)
      別所裕紀、岩越-浮穴栄子、古満芽久美、前嶋 翔、谷内秀輔、橘 哲也、浮穴和義
    • Organizer
      第65回日本動物学会中国四国支部大会
    • Place of Presentation
      徳島
    • Related Report
      2013 Annual Research Report
  • [Presentation] Novel genes encoding neuropeptides in the chicken hypothalamus.

    • Author(s)
      Ukena K, Iwakoshi-Ukena E, Taniuchi S, Maejima S, Bessho Y, Masuda K, Furumitsu M, Tachibana T.
    • Organizer
      17th International Congress of Comparative Endocrinology
    • Place of Presentation
      スペイン
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニワトリの視床下部における新規神経ペプチド産生細胞の形態学的解析

    • Author(s)
      別所裕紀、岩越-浮穴栄子、谷内秀輔、古満芽久美、前嶋 翔、浮穴和義
    • Organizer
      第84回日本動物学会大会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
  • [Presentation] ラットにおける視床下部新規神経ペプチドの電子顕微鏡観察

    • Author(s)
      前嶋 翔、岩越-浮穴栄子、佐藤慧太、坂本浩隆、坂本竜哉、浮穴和義
    • Organizer
      第84回日本動物学会大会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
  • [Presentation] 視床下部で見出した新規神経ペプチド前駆体遺伝子の進化的保存性

    • Author(s)
      谷内秀輔、岩越-浮穴栄子、浮穴和義
    • Organizer
      第84回日本動物学会大会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
  • [Presentation] ラット視床下部新規神経ペプチドの脳室内慢性投与による影響

    • Author(s)
      近藤邦裕、鹿野健史朗、谷内秀輔、別所裕紀、大口悦宏、益田恵子、大山晴香、前嶋 翔、岩越栄子、浮穴和義
    • Organizer
      第38回日本比較内分泌学会学術集会
    • Place of Presentation
      宮崎
    • Related Report
      2013 Annual Research Report
  • [Presentation] 視床下部新規遺伝子がコードしている神経ペプチドの効率的産出方法の確立

    • Author(s)
      益田恵子
    • Organizer
      第83回日本動物学会大会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] 視床下部で発見した新規遺伝子は分泌性ペプチドの前駆体か?

    • Author(s)
      古満芽久美
    • Organizer
      第83回日本動物学会大会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] ラットの視床下部新規遺伝子がコードしている神経ペプチドの摂食行動への影響

    • Author(s)
      鹿野健史朗
    • Organizer
      第37回日本比較内分泌学会大会
    • Place of Presentation
      福井
    • Related Report
      2012 Annual Research Report
  • [Presentation] 異なるエネルギー代謝状態におけるラットの視床下部新規遺伝子mRNA発現解析

    • Author(s)
      近藤邦裕
    • Organizer
      第37回日本比較内分泌学会大会
    • Place of Presentation
      福井
    • Related Report
      2012 Annual Research Report
  • [Presentation] 鳥類の視床下部で発見した新規神経ペプチド前駆体遺伝子の機能解析―摂食調節に着目して―

    • Author(s)
      浮穴和義
    • Organizer
      第46回日本味と匂学会 シンポジウム
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/ukena/

    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] ポリペプチド, 核酸, 発現ベクター, 形質転換体, 成長ホルモン発現抑制剤, 非ヒト動物及び非ヒト動物の作製方法2013

    • Inventor(s)
      浮穴和義, 岩越栄子, 谷内秀輔, 鹿野健史朗, 近藤邦裕
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-219578
    • Filing Date
      2013-10-22
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] ポリペプチド、核酸、発現ベクター、形質転換体、成長ホルモン発現抑制剤、非ヒト動物及び非ヒト動物の作製方法2013

    • Inventor(s)
      浮穴和義、岩越栄子、谷内秀輔、鹿野健史朗、近藤邦裕
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-219578
    • Filing Date
      2013-10-22
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] ポリペプチド, ポリペプチドの製造方法, 摂食調節組成物および摂食量の調節方法2011

    • Inventor(s)
      浮穴和義, 岩越栄子, 益田恵子, 古満芽久美
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2011-248006
    • Filing Date
      2011-11-11
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] ポリペプチド, 摂食促進組成物および摂食量の促進方法2011

    • Inventor(s)
      浮穴和義, 岩越栄子
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2011-065216
    • Filing Date
      2011-03-24
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] ポリペプチド、ポリペプチドの製造方法、摂食調節組成物および摂食量の調節方法2011

    • Inventor(s)
      浮穴和義
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Number
      2011-248006
    • Filing Date
      2011-11-11
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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